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EC number: 257-104-6 | CAS number: 51277-96-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Biodegradation in water: screening test, toxicity control is used to derive effect concentration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: EU method C.4-D
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Principles of method if other than guideline:
- From biodegradation in water: screening test, toxicity control is used to derive effect concentration
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item was a 5% aquatic dispersion.The concentration in the test assays were 2000 mg dispersion per litre mineral test medium and 400 mg dispersion per litre mineral test medium, respectively. 2000 mg test item correspond to 290 mg ThOD, 400 mg test item correspond to 58 mg ThOD. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Fresh samples of activated sludge are withdrawn on June 10th, 2014 from the sewage treatment plant Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany, which is mainly fed with municipal wastewater. Since it was not necessary, the samples were not washed with mineral medium after the arrival at the laboratory but kept aerobic until use.The concentration used in the test was 29.6 mg dry mass/litre (7.39 mg dry mass/250 mL).
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Test temperature:
- 22 °C ± 1°C
- pH:
- 7.4 ± 0.2 at test start
- Nominal and measured concentrations:
- test item at 2000 mg per litre + reference item at 100 mg per litre
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium:
( a )
KH2PO4: 8.50 g/L
K2HPO4: 21.75 g/L
Na2HPO4 x 12 H2O: 67.13 g/L
NH4Cl: 0.50 g/L
adjusted to 7.4 ± 0.2 with H2SO4 (50 g/L)
( b )
CaCl2 x 2 H2O: 36.40 g/L
( c ) MgSO4 x 7 H2O: 22.50 g/L
( d ) FeCl3 x 6 H2O: 0.25 g/L.
The mineral medium applied in the test contained 10 mL/L of mineral stock solution a and 1 mL/L of the mineral stock solution b–d, respectively.
- Test temperature: 22 °C
- pH: 7.4 +/- 0.2
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Test vessel: 500 mL glass vessels, fill volume 250 mL
- Aeration: yes
- Number of culture flasks/concentration: 2 vessels containing test item (2000 mg/L) and inoculum; 2 vessels containing test item (400 mg/L) and inoculum; 2 vessels containing only inoculum; 2 vessels containing test item (2000 mg/L) and a sterilising agent
- Method used to create aerobic conditions: The suspension was aerated during the whole test.
- Measuring equipment: SAPROMAT respirometer (VOITH Inc.).
SAMPLING
- Sampling frequency: The measurement and recording of the oxygen demand was carried out continuously
CONTROL AND BLANK SYSTEM
- Inoculum blank: The blank control consists of inoculated mineral medium only
- Abiotic sterile control: An abiotic control containing test item at 2000 mg per litre sterilized mineral test medium was applied. The assay was sterilized by adding HgCl2.
- Toxicity control: A toxicity control containing test item at 2000 mg per litre and reference item at 100 mg per litre mineral test medium was applied.
STATISTICAL METHODS:
Theoretical Oxygen Demand (ThOD):
The Theoretical Oxygen Demand (ThOD) was calculated on the basis of the sum formula of the test and reference item by:
ThOD [g/g] = 16 * (2 C + 1/2 H + 5/2 N + 1/2 Na – O) / Molecular weight.
The ThOD values for Na-benzoate, the test item and the toxicity control were determined as follows:
ThODPC-2014-536: 0.145 mg O2/mg test item
ThODNa-Benzoate: 1.665 mg O2/mg reference item
ThODtoxicity control: 0.218 mg O2/mg substance mixture
Biochemical Oxygen Demand (BOD):
The Biochemical Oxygen Demand (BOD) was calculated on the basis of the test raw data by BOD [mg/mg] = mg O2 uptake corrected by blank per mg test item.
The percent degradation was calculated according to the following formula:
Dt = [(Ct –Cb) / ThOD] x 100Dt: degradation (%) at time t;
Ct: mean oxygen consumption (mg/L) in the test suspension at time t;
Cb: mean oxygen consumption (mg/L) in the blanks at time t;
ThOD: Theoretical oxygen demand of the test suspension (mg/L). - Reference substance (positive control):
- no
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: comparison of procedural and toxicity control
- Details on results:
- The biodegradation of the item mixture in the toxicity control was found to be 31.1% after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 2000 mg/L, as the biodegradation in the toxicity control was higher than 25% within 14 days of incubation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of C16 Alkylamidopropyltrimethylammonium Chloride to microorganisms was investigated during a ready biodegradation study. The test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 2000 mg/L.
- Executive summary:
The toxicity of C16 Alkylamidopropyltrimethylammonium Chloride to microorganisms was investigated during a ready biodegradation study according to EU Method C.4-D (2008) and OECD guideline 301 F (1992), Manometric Respirometry Test over a period of 28 days and using an inoculum obtained from activated sludge from a predominantly domestic sewage treatment plant.
Inoculum blank, procedural/functional control with the reference substance Sodium benzoate and a toxicity control with 2000 mg/L test item and 100 mg/L reference item Sodium benzoate were performed.
The reference item degraded normally (91.6% by within 14 days). By the end of the test, the reference item was degraded to an average of 96.4%.
The biodegradation of the item mixture in the toxicity control was found to be 31.1% after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 2000 mg/L, as the biodegradation in the toxicity control was higher than 25% within 14 days of incubation.
Reference
Description of key information
14 d NOEC = 2000 mg/L; toxicity control from OECD Guideline 301 F (1992); RL1; GLP
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 2 000 mg/L
Additional information
The toxicity of C16 Alkylamidopropyltrimethylammonium Chloride (95% a.i.) to microorganisms was investigated during a ready biodegradation study according to EU Method C.4-D (2008) and OECD guideline 301 F (1992), Manometric Respirometry Test over a period of 28 days and using an inoculum obtained from activated sludge from a predominantly domestic sewage treatment plant.
Inoculum blank, procedural/functional control with the reference substance Sodium benzoate and a toxicity control with 2000 mg/L test item and 100 mg/L reference item Sodium benzoate were performed.
The reference item degraded normally (91.6% by within 14 days). By the end of the test, the reference item was degraded to an average of 96.4%.
The biodegradation of the item mixture in the toxicity control was found to be 31.1% after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 2000 mg/L, as the biodegradation in the toxicity control was higher than 25% within 14 days of incubation.
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