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EC number: 701-354-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2018-05-21 to 2018-05-28 with the definitive exposure phase from 2018-05-22 to 2018-05-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Determination of the test item
All concentration levels and the control were analytically verified via LC-QTOF at the start (0 hours) and at the end of the exposure (72 hours) with algae. The samples were analysed with a LC-QTOF method, implemented under non-GLP and documented in the GLP raw data. The method was validated. - Vehicle:
- no
- Details on test solutions:
- Water Accommodated Fraction (WAF)
In view of the difficulties associated with the evaluation of aquatic toxicity of poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed. An approach endorsed by several important regulatory authorities in the EU and elsewhere (ECETOC 1996 and OECD 2000) is to expose the organisms to a Water Accommodated Fraction (WAF) of the test item in cases where the test item is a complex mixture and is poorly soluble in water and in the permitted solvents. Using this approach, aqueous media were prepared by mixing the test item with water for a prolonged period sufficient to ensure equilibration between the test item and the water phase. After completion of mixing and following a settlement period, the test item phase was separated by siphon and the test organisms were exposed to the aqueous phase, the WAF (which may contain dissolved and/or suspended and/or emulsified fractions of the test item mixture). Exposure is expressed in terms of the original concentration of the test item in water at the start of the mixing period (loading rate) irrespective of the actual concentration of the test item in the WAF.
Preparation of the water accommodated fraction
Five water accommodated fractions (WAF) were prepared with a nominal loading of the test item of 0.100 – 0.316 – 1.00 – 3.16 – 10.0 mg/L (spacing factor √10). The loading levels are based on the results of a preliminary range finding test (non-GLP, open system).
For the loading levels 1.00, 3.16 and 10.0 mg/L an appropriate amount of the test item was pipetted onto the surface of the dilution water. For this purpose, the density was taken into account. These dispersions were shaken for 24 hours with 20 rpm at room temperature. After a separation phase of 1 hour, the aqueous phase or WAFs were removed by siphoning (from the approximate centre of the glass flask). The resulting water accommodated fractions (WAF) were used in the test. The loading levels 0.100 and 0.316 mg/L were prepared by diluting the WAF with a loading of 1.00 mg/L.
Test loading
Per definition of the WAF, all terms related to concentration level are given as loading level. Since the analytical recoveries were inside the nominal range (±20%), these correspond to the actual test item concentrations.
Control
Six replicates (without test item) were exposed under the same conditions as the item replicates. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata HINDÁK
- Strain: SAG 61.81
- Source (laboratory, culture collection): SAG, Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, D-37073 Göttingen
- Age of inoculum (at test initiation): A three day old preculture, prepared in dilution water, was used as inoculum.
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 2567 – 5130 lux for 24 h per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- Not specified
- Test temperature:
- min.: 22.0 max.: 24.0 mean value: 23.0
- pH:
- Nominal test item loading pH-value
[mg/L] Start; 0 hours End; 72 hours
10.0 7.70 8.03
3.16 7.75 8.23
1.00 7.75 8.63
0.316 7.87 8.88
0.100 7.96 8.87
Control 8.10 8.90 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater conditions
- Nominal and measured concentrations:
Nominal test item concentrations: 0.1, 0.316, 1, 3.16 and 10 mg/L
See any other information on results- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 5 x 103 - 104 cells/mL. Actual: 5523 cells/mL
- Control end cells density: Mean 2161234 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Composition of Dilution Water according to the guideline
Component Concentration [mg/L]
NH4Cl 15
MgCl2 x 6 H2O 12
CaCl2 x 2 H2O 18
MgSO4 x 7 H2O 15
KH2PO4 1.6
FeCl3 x 6 H2O 0.064
Na2EDTA x 2 H2O 0.1
H3BO3 0.185
MnCl2 x 4 H2O 0.415
ZnCl2 3 x 10^-3
Na2MoO4 x 2 H2O 7 x 10^-3
CoCl2 x 6 H2O 1.5 x 10^-3
CuCl2 x 2 H2O 1 x 10^-5
NaHCO3 50
pH 8.1 ± 0.2
This medium had a nominal hardness of 0.24 mmol Ca+Mg/L.
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: Approximately 4440 to 8880 lux, corresponding to 60 to 120 µE ∙ m-2 ∙ s-1, mean value: 6105 lux, range of the measured values: 5205 to 6744
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
-Chlorophyll a-fluorescence
The cell density was measured daily via Chlorophyll a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal. No self-fluorescence was found in the preliminary range finding test at the concentration levels of 10.0 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- (Potassium dichromate)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 29.7% A.I.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CI (0.448 - 0.891)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.203 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 29.7% a.i.
- Basis for effect:
- other: Yield Inhibition
- Remarks on result:
- other: CI (0.133 - 0.292)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.316 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield Inhibition
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield Inhibition
- Remarks on result:
- other: CI (2.00 - 3.26)
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: All test solutions were visually clear throughout the exposure period (possible turbidity related to algae growth not taken into account).
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- The toxicity of potassium dichromate (SIGMA-ALDRICH, batch number MKBV0900V, purity 99.0 %, CAS RN 7778-50-9) to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined over a period of 72 hours from 2018-04-09 to 2018-04-12, with headspace. The reference item toxicity is in the valid range following test facility SOPS.
EC50-Values of the Reference Item
based on nominal concentrations mg/L, (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Growth Rate inhibition
ErC50 0.574 0.752 ± 0.554
95 % confidence interval 0.549 – 0.596
Yield inhibition
EyC50 0.337 0.399 ± 0.294
95 % confidence interval 0.313 – 0.345
SD = Standard deviation - Reported statistics and error estimates:
- Since the two main ingredients of the test item are considered to be soluble and analytical recoveries were inside the nominal range throughout the exposure phase, loading rate and actual test item concentration are considered to be interchangeable in this study. Therefore, all effect values are reported based on concentration of the test item.
EC-values and statistical analyses
EC10-, EC20- and EC50-value with confidence intervals of growth rate and yield inhibition after 72 hours was calculated sigmoidal dose response.
NOEC, LOEC and statistical analyses
The NOEC and LOEC were determined by calculation of statistically significant differences of growth rates and yield. As a standard, One Way Analysis of Variance (ANOVA) and DUNNETT’s test were used for NOEC/LOEC calculations. When running a One Way Analysis of Variance, a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance tests are 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is =0.05. The Normality Test failed for the calculation of growth rate. Therefore, data was transformed (Y square).
Software
The data for the tables in this report were computer-generated and have been rounded for presentation from the full derived data. Consequently, if calculated manually based on the given data minor deviations may occur from these figures.
Calculations were carried out using software
•Excel, MICROSOFT CORPORATION
•SigmaPlot, SPSS INC.
•GraphPad Prism, GRAPHPAD SOFTWARE, INC. - Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, Sodium cocopropylenediamine propionate CAS no. 97659-50-2 was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values (based on nominal test item loadings*): The EC50-values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were > 10.0 mg/L (>2.97 mg a.i./L) and 2.51 (2.00 – 3.26) mg/L, respectively. The 72h EC10-value for growth is 2.22 mg/L (0.66 mg a.i./L)
All effect levels are based on nominal test item loadings*.
* Due to the high water solubility of the test item, loading rate and actual test item concentration are considered to be interchangeable. - Executive summary:
The toxicity of Sodium cocopropylenediamine propionate (Batch no.:48724) to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 and Council Regulation (EC) No. 266/2016/Method C.3 from 2018-05-21 to 2018-05-28 with the definitive exposure phase from 2018-05-22 to 2018-05-25. The aim of the study was the determination of NOEC, LOEC, EC10- , EC20- and EC50-values of growth rate and yield over a period of 72 hours.
The test item Sodium cocopropylenediamine propionate is a yellow liquid, which is soluble in water.The study was conducted under static conditions with an initial cell density of 5523 cells/mL. Five water accommodated fractions (WAF) were prepared in a geometrical series with a factor of √10: 0.100 – 0.316 – 1.00 – 3.16 – 10.0 mg/L. For the loading levels 10.0 – 3.16 – 1.00 mg/L, an appropriate amount of the test item was pipetted onto the water surface (density of the test item taken into account). These dispersions were shaken at 20 rpm for 24 hours at room temperature. After a separation phase of 1 hour at room temperature, the WAFs were removed by siphoning and used in the test. The loading levels of 0.316 – 0.100 mg/L were prepared by dilution of the WAF with a nominal loading of 1.00 mg/L. The environmental conditions were within the acceptable limits. The test solutions were clear throughout the test period.
All loading levels and the control were analytically verified by LC-QTOF at the start and the end of the exposure.The concentration levels of the test item Sodium cocopropylenediamine propionate were between 94% and 114% of the nominal value at the start of the exposure (0 hours) and between 86% and 112% of the nominal value at the end of the exposure (72 hours).
All effect values given are based on the nominal loading* of the test item.
NOEC, LOEC, EC-values and 95% Confidence Intervals ofSodiumcocopropylenediaminepropionate (0 - 72 hours)
based on nominal loading of the test item* [mg/L]
Growth Rate Inhibition
Nominal test item loading* [mg/L]NOEC
1.00
LOEC
3.16
ErC10
2.22 (1.51 – 3.00)
ErC20
4.36 (3.39 – 6.11)
ErC50
> 10.0
Yield Inhibition
Nominal test item loading* [mg/L]
NOEC
0.316
LOEC
1.00
EyC10
0.684 (0.449 – 0.986)
EyC20
1.03 (0.733 – 1.36)
EyC50
2.51 (2.00 – 3.26)
* Due to the high water solubility of the test item, loading rate and actual test item concentration are considered to be interchangeable
Reference
Cell Densities
Nominal test item loading |
Replicate |
Cell density [cells/mL] |
|||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
10.0 |
1 |
5523 |
13909 |
48816 |
228786 |
2 |
5523 |
15566 |
48801 |
246657 |
|
3 |
5523 |
11153 |
31196 |
122397 |
|
Mean |
5523 |
13543 |
42938 |
199280 |
|
3.16 |
1 |
5523 |
22488 |
120542 |
992088 |
2 |
5523 |
31821 |
101196 |
915558 |
|
3 |
5523 |
26563 |
127151 |
824063 |
|
Mean |
5523 |
26957 |
116296 |
910570 |
|
1.00 |
1 |
5523 |
27857 |
141032 |
1459807 |
2 |
5523 |
31779 |
183310 |
1668331 |
|
3 |
5523 |
35330 |
226882 |
2054561 |
|
Mean |
5523 |
31655 |
183741 |
1727566 |
|
0.316 |
1 |
5523 |
36278 |
246791 |
2329429 |
2 |
5523 |
31492 |
322930 |
2112952 |
|
3 |
5523 |
27771 |
253547 |
2238749 |
|
Mean |
5523 |
31847 |
274423 |
2227043 |
|
0.100 |
1 |
5523 |
33497 |
325281 |
2111905 |
2 |
5523 |
37026 |
328393 |
2464352 |
|
3 |
5523 |
25990 |
300972 |
2326307 |
|
Mean |
5523 |
32171 |
318215 |
2300855 |
|
Control |
1 |
5523 |
31018 |
291298 |
2239764 |
2 |
5523 |
32263 |
297275 |
2356077 |
|
3 |
5523 |
33660 |
345361 |
2142218 |
|
4 |
5523 |
22972 |
234628 |
2034647 |
|
5 |
5523 |
28484 |
218843 |
2188250 |
|
6 |
5523 |
33935 |
275029 |
2006445 |
|
Mean |
5523 |
30389 |
277072 |
2161234 |
Evaluation after 72 hours
Statistically
significant differences of growth rates and yield compared to
control
values are marked (+), not significant differences are marked (-).
Nominal test item loading |
Replicate |
Growth rate |
Inhibition of growth rate |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
10.0 |
1 |
|
1.24 |
38 |
|
223263 |
90 |
2 |
|
1.27 |
36 |
|
241134 |
89 |
|
3 |
|
1.03 |
48 |
|
116874 |
95 |
|
Mean |
(+) |
1.18 |
41 |
(+) |
193757 |
91 |
|
3.16 |
1 |
|
1.73 |
13 |
|
986565 |
54 |
2 |
|
1.70 |
14 |
|
910035 |
58 |
|
3 |
|
1.67 |
16 |
|
818540 |
62 |
|
Mean |
(-) |
1.70 |
15 |
(+) |
905047 |
58 |
|
1.00 |
1 |
|
1.86 |
7 |
|
1454284 |
33 |
2 |
|
1.90 |
4 |
|
1662808 |
23 |
|
3 |
|
1.97 |
1 |
|
2049038 |
5 |
|
Mean |
(-) |
1.91 |
4 |
(+) |
1722043 |
20 |
|
0.316 |
1 |
|
2.02 |
-1 |
|
2323906 |
-8 |
2 |
|
1.98 |
0 |
|
2107429 |
2 |
|
3 |
|
2.00 |
-1 |
|
2233226 |
-4 |
|
Mean |
(-) |
2.00 |
-1 |
(-) |
2221520 |
-3 |
|
0.100 |
1 |
|
1.98 |
0 |
|
2106382 |
2 |
2 |
|
2.03 |
-2 |
|
2458829 |
-14 |
|
3 |
|
2.01 |
-1 |
|
2320784 |
-8 |
|
Mean |
(-) |
2.01 |
-1 |
(-) |
2295332 |
-6 |
|
Control |
1 |
|
2.00 |
|
|
2234241 |
|
2 |
|
2.02 |
|
|
2350554 |
|
|
3 |
|
1.99 |
|
|
2136695 |
|
|
4 |
|
1.97 |
|
|
2029124 |
|
|
5 |
|
1.99 |
|
|
2182727 |
|
|
6 |
|
1.97 |
|
|
2000922 |
|
|
Mean |
|
1.99 |
|
|
2155711 |
|
Section-by-Section and Average Specific Growth Ratesof the Control Group
(0 – 72 hours)
|
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 hours) |
SD ± |
CV |
Mean CV [%] |
||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
1.73 |
2.24 |
2.04 |
2.00 |
0.259 |
12.9 |
14.58 |
2 |
1.77 |
2.22 |
2.07 |
2.02 |
0.232 |
11.5 |
||
3 |
1.81 |
2.33 |
1.83 |
1.99 |
0.296 |
14.9 |
||
4 |
1.43 |
2.32 |
2.16 |
1.97 |
0.479 |
24.3 |
||
5 |
1.64 |
2.04 |
2.30 |
1.99 |
0.333 |
16.7 |
||
6 |
1.82 |
2.09 |
1.99 |
1.97 |
0.140 |
7.11 |
||
|
|
|
Mean |
2.06 |
|
|
||
|
|
|
SD ± |
0.163 |
|
|||
|
|
|
CV [%] |
7.80 |
|
SD = Standard deviation CV = Coefficient of variation
Measured Exposure Concentrations during the Definitive Test
The concentrations of Sodiumcocopropylenediamine propionate were determined in fresh media (0 hours) and old media (72 hours) of all tested concentration levels and the control via LC-QTOF.
The measured concentrations of the test item were between 94% and 114% of the nominal value at the start of the exposure (0 hours). At the end of the exposure (72 hours) the concentration of the test item were between 85% and 119%.
Measured Concentrations and Percent of Nominal Concentration of theTest Item in Fresh Medium (0 hours) with Algae
Sampling date |
0 hours |
72 hours |
|||
Nominal loading of the |
Sodium cocopropylenediamine propionate (C12 Tripropionate) |
||||
test item [mg/L] |
Active ingredient [mg a.i./L] |
Meas. conc. [mg a.i./L] |
[%] |
Meas. conc. [mg a.i./L] |
[%] |
10.0 |
1.90 |
1.88 |
99 |
1.83 |
96 |
3.16 |
0.601 |
0.593 |
99 |
0.514 |
86 |
1.00 |
0.190 |
0.188 |
99 |
0.172 |
91 |
0.316 |
0.0601 |
0.0607 |
101 |
0.0563 |
94 |
0.100 |
0.0190 |
0.0216 |
114 |
0.0213 |
112 |
Control |
< LOQ |
< LOQ |
Meas. Conc. = measured concentration of the active ingredient of the test item
(dilution and weighing factor taken into account)
a.i. = active ingredient (C12-Tripropionate)
% = percentage of the nominal concentration of the active ingredient
LOQ = limit of quantification of the analytical method (0.08 mg test item/L)
Measured Concentrations and Percent of Nominal Concentration of theTest Item in Fresh Medium (0 hours) with Algae
Sampling date |
0 hours |
72 hours |
|||
Nominal loading of the |
Sodium cocopropylenediamine propionate (C14 Tripropionate) |
||||
test item [mg/L] |
Active ingredient [mg a.i./L] |
Meas. conc. [mg a.i./L] |
[%] |
Meas. conc. [mg a.i./L] |
[%] |
10.0 |
0.475 |
0.454 |
96 |
0.466 |
98 |
3.16 |
0.150 |
0.142 |
94 |
0.127 |
85 |
1.00 |
0.0475 |
0.0458 |
97 |
0.0440 |
93 |
0.316 |
0.0150 |
0.0142 |
94 |
0.0141 |
94 |
0.100 |
0.00475 |
0.00448 |
94 |
0.00566 |
119 |
Control |
< LOQ |
< LOQ |
Meas. Conc. = measured concentration of the active ingredient of the test item
(dilution and weighing factor taken into account)
a.i. = active ingredient (C14-Tripropionate)
% = percentage of the nominal concentration of the active ingredient
LOQ = limit of quantification of the analytical method (0.08 mg test item/L)
Since the measured concentrations of the two main constituents of the test item were inside the nominal range throughout the exposure period, loading rate and actual test item concentration are considered to be interchangeable
Description of key information
The test item has an active ingredient content of 29.7%. The ErC10 and ErC50 based on active ingredient are 0.66 and >2.97 mg/L respectively
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2.97 mg/L
- EC10 or NOEC for freshwater algae:
- 0.66 mg/L
Additional information
The toxicity of Sodium cocopropylenediamine propionateto the freshwater green algaPseudokirchneriella subcapitatawas determined according to the principles of OECD 201 andEC method C.3.The aim of the study was the determination of NOEC, LOEC, EC10- , EC20- and EC50-values of growth rate and yield over a period of 72 hours.
Sodium cocopropylenediamine propionateis a light yellow solid substance, which is soluble in water.The study was conducted under static conditions with an initial cell density of 5523 cells/mL. Five water accommodated fractions (WAF) were prepared in a geometrical series with a factor of√10: 0.100 – 0.316 – 1.00 – 3.16 – 10.0 mg/L. For the loading levels 10.0 – 3.16 – 1.00 mg/L, an appropriate amount of the test item was pipetted onto the water surface (density of the test item taken into account). These dispersions were shaken at 20 rpm for 24 hours at room temperature. After a separation phase of 1 hour at room temperature, the WAFs were removed by siphoning and used in the test. The loading levels of 0.316 – 0.100 mg/L were prepared by dilution of the WAF with a nominal loading of 1.00 mg/L. The environmental conditions were within the acceptable limits. The test solutions were clear throughout the test period.
All concentration levels and the control were analytically verified by LC-QTOF at the start and the end of the exposure. The concentration levels of the test item
Sodium cocopropylenediamine propionate were between 99% and 114% of the nominal value at the start of the exposure (0 hours).
All effect values given are based on the nominal loading of the test item.
NOEC, LOEC, EC-values and 95% Confidence Intervals ofSodiumcocopropylenediamine propionate (0 - 72 hours)
based on nominal loading of the test item [mg/L]
|
Growth Rate Inhibition |
NOEC |
3.16 |
LOEC |
10.0 |
ErC10 |
2.22 (1.51 – 3.00) |
ErC20 |
4.36 (3.39 – 6.11) |
ErC50 |
> 10.0 |
|
Yield Inhibition Nominal test item loading [mg/L] |
NOEC |
0.316 |
LOEC |
1.00 |
EyC10 |
0.684 (0.449 – 0.986) |
EyC20 |
1.03 (0.733 – 1.36) |
EyC50 |
2.51 (2.00 – 3.26) |
The test item has an active ingredient content of 29.7%. The ErC10 and ErC50 based on active ingredient are 0.66 and >2.97 mg/L respectively
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