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Diss Factsheets
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EC number: 944-490-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- phototoxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 432 (In Vitro 3T3 NRU Phototoxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of method:
- in vitro
- Endpoint addressed:
- other: phototoxicity
Test material
- Reference substance name:
- 1-hydroxyoctan-2-yl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12-dienoate; octane-1,2-diol
- EC Number:
- 944-490-2
- Molecular formula:
- unknown
- IUPAC Name:
- 1-hydroxyoctan-2-yl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12-dienoate; octane-1,2-diol
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Yellow oil, soluble at 10% (w/v) in paraffin oil.
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- In vitro environmental conditions:
Mouse embryo fibroblasts (3T3 balb/c) were cultured during 24h in a DMEM medium containing 4.5g/L of glucose, 4 mM of glutamine and 110 mg/mL of sodium pyruvate with 10% of newborn calf serum, 100 UI/mL of penicillin and 100 μg/mL of streptomycin. Hygrometry >90%, temperature at 37°C.
Administration / exposure
- Vehicle:
- paraffin oil
- Analytical verification of doses or concentrations:
- no
- Details on analytical verification of doses or concentrations:
- no data
- Duration of treatment / exposure:
- 1 hour
- Frequency of treatment:
- not specified
- Post exposure period:
- 18 to 22 hours post incubation period
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0.001 other: %
- Dose / conc.:
- 0.002 other: %
- Dose / conc.:
- 0.003 other: %
- Dose / conc.:
- 0.006 other: %
- Dose / conc.:
- 0.013 other: %
- Dose / conc.:
- 0.025 other: %
- Dose / conc.:
- 0.05 other: %
- Dose / conc.:
- 0.1 other: %
- No. of animals per sex per dose:
- not applicable
- Details on study design:
- Positive control: chlorpromazine hydrochloride; Negative control: EBSS without Ca2+ nor Mg2+ (used to solubilize the positive control) ;Vehicule control: paraffin oil
Test substance concentrations were prepared from a 1% mother solution. Preparation was extemporaneous.
Two 96-well plates of cells were treated at 8 different concentrations of test item (100 μL/well) for 1 hour. One plate was not exposed to UVA while the other was exposed to UVA at a final dose of 5J/cm². Cells were rinsed and plates were post-incubated for 18-22 hours. Cytotoxicity was then assessed through neutral red uptake technique. Cellular viability, IC50 and PIF (photoirritation factor) were calculated.
Examinations
- Examinations:
- Reading of the optical densities at 540 nm to determine % of viability, then IC50 and PIF.
- Positive control:
- IC50 (without UVA) = 10.0 µg/mL
IC50 (with UVA) = 0.6 µg/mL
PIF = 16.7
Results and discussion
- Details on results:
- Validity criteria of the study were accepted.
Results for the test substance were: IC50 (without UVA) = 0.025 µg/ml and IC50 (with UVA) = 0.025 µg/ml.
The PIF (photoirritation factor), calculated from both IC50, was 1.0. According to the classification, test substance was not phototoxic.
Applicant's summary and conclusion
- Conclusions:
- In an OECD 3T3 NRU phototoxicity test, test substance was non phototoxic at all tested concentrations.
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