Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 619-642-3 | CAS number: 106-78-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-01-29 to 2001-02-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Principles of method if other than guideline:
- Some test fish had a somewhat smaller size than the one recommended in the test guideline for the zebra fish.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-{2-[(2-chloroacetyl)oxy]ethoxy}ethyl 2-chloroacetate
- Cas Number:
- 106-78-5
- Molecular formula:
- C8H12O5Cl2
- IUPAC Name:
- 2-{2-[(2-chloroacetyl)oxy]ethoxy}ethyl 2-chloroacetate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule of chemical analysis:
Stock solution: at 0 hours
Control: 0, 24, 48, 72 and 96 hours
Test concentrations: at 0, 24, 48, 72 and 96 hours
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Pretreatment of the test substance:
A stock solution was prepared to give the desired series of test concentrations. To achieve this 1000.2 mg of the test substance were added to 10 litres ofdilution water, then treated with an ultra turrax for 60 seconds and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Strain: Zebra fish (Brachydania reria HAMILTON-BUCHANAN)
- Source: Bio International B. V. (Netherlands)
- Age (mean and range, SD): Date ofbirth: March 9, 2000; Date supplied: July 25,2000
- Length (length definition, mean, range and SD): mean standard length (n = 60): 2.7 cm (S.D. = 0.29 cm)
ACCLIMATION
- Acclimation period: Stock held since July 25,2000 and acclimatised to the test conditions since then
- Acclimation conditions (same as test or not): not specified
- Health during acclimation (any mortality observed): Mortality <2%
- Feeding: Commercial fish food, daily. Discontinued 24 h prior to test
FEEDING DURING TEST
no feeding during exposure period
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- The hardness of the dilution water used was 13.1 °dH (= 233.8 mg/I CaC03).
- Test temperature:
- 20-24°C +- 1°C
- pH:
- 6.8 - 7.9
- Dissolved oxygen:
- 8.2 - 8.9 mg/L
- Salinity:
- n.a.
- Conductivity:
- no data
- Nominal and measured concentrations:
- See table 1
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria holding 5 I oftest media
- Type (delete if not applicable): open
- Aeration: gentle aeration via narrow glass tubes
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.70g bw (wet weight) per litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared according to ISO 7346
- Total organic carbon: see table 1
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16h lieght : 8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, subjective assessments were made on the type and incidence ofsub-lethal effects compared with control fish.
TEST CONCENTRATIONS
6.3, 12.5, 25, 50 and 100 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 13.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 52.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Chemical analysis
Time
0h
24 h
48 h
72 h
96 h
Control
TOC values [mg/L]
<2
<2
<2
3
3
Nominal concentration [mg/L]: 6.3
TOC values [mg/L]
3
3
4
3
4
measured test substance [mg/L]
8.1
8.1
10.8
8.1
10.8
Nominal concentration [mg/L]: 12.5
TOC values [mg/L]
5
6
6
5
3
measured test substance [mg/L]
13.5
16.2
16.2
13.5
8.1
Nominal concentration [mg/L]: 25
TOC values [mg/L]
10
11
13
11
11
test substance [mg/L]
27
29.7
35.1
29.7
29.7
Nominal concentration [mg/L]: 50
TOC values [mg/L]
19
20
measured test substance [mg/L]
51.3
54
Nominal concentration [mg/L]: 100
TOC values [mg/L]
37
40
measured test substance [mg/L]
99.9
108
Table 2: Analysis of mortality data give the following results:
Time [h]
LC 0 [mg/L]
LC 100 [mg/L]
Geometric mean
LC 0 / LC 100 [mg/L]
2
>= 99.9
> 99.9
-
24
14.9
52.7
28.0
48
15.3
52.7
28.4
72
14.9
52.7
28.0
96
13.5
52.7
26.7
Highest test concentration resulting in 0% mortality (LC 0 96h): 13.5 mg/L
Lowest test concentration resulting in 100% mortality (LC 100 96h): 52.7 mg/L
All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 99.9 - 128.6% of nominal values at 0 hours, from 108.0 - 129.6% of nominal values at 24 hours, from 129.6 -171.4% ofnominal values at 48 hours, from 108.0 -128.6% of nominal values at 72 hours, and from 64.8 - 171.4% of nominal values at 96 hours, respectively.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions a LC 0 96 h of 13.5 mg/L and a LC 100 96h of 52.7 mg/L was determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.