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EC number: 412-260-6 | CAS number: 52658-19-2 MONO 442
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 27, 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Sincethe test substance was hardly soluble in water test concentrations were prepared separately in Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA).
Two 1 litre test bottles were filled with 250 ml of test substance mixtures with initial loading rates of 200 mg/l. These mixtures were stirred in closed bottles for 26 hours. Subsequently, 16 ml synthetic sewage feed, 200 ml sludge and Milli-RO water up to 500 ml were added resulting in a loading rate of 100 mg/l. This loading rate was tested in duplicate. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (4.3 g/l of sludge, as used for the test). The pH was 6.2 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After the exposure period, oxygen consumption was measured and recorded for approximately 10 minutes.
Measurement of pH. - Hardness:
- not applicable
- Test temperature:
- 18.2 - 19.5°C
- pH:
- 7.2 - 7.5
- Dissolved oxygen:
- 7.3 - 9.0 mg/l
- Salinity:
- not applicable
- Nominal and measured concentrations:
- a loading rate of 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, approximately 300 ml oxygen bottles (measuring) and 1 litre test bottles (exposure).
- Aeration: continuously during exposure
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 200 ml activated sludge
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: none
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The respiration rate from each vessel, in mg O2/l/hr, was calculated from the linear part of the respiration curve, which was generally between 2.5 and 6.5 mg O2/l.
If applicable the EC50 value can be determined. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Slight inhibition of respiration rate of the sludge was recorded at 100 mg test substance per litre. The duplicate measurement confirmed the result of the first measurement (values of 12 and 18% inhibition only were recorded). Therefore, no further testing was needed.
Hence, the EC50 of the test substance exceeded a loading rate of 100 mg/l (based on nominal concentrations). - Results with reference substance (positive control):
- Three concentrations were tested: 5.0, 12 and 30 mg/l.
Results:
Conc. 5.0 mg/l - respir. inhibition 40 %
Conc. 12.0 mg/l - respir. inhibition 77 %
Conc. 30.0 mg/l - respir. inhibition 91 % - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the EC50 of the test substance exceeded a loading rate of 100 mg/l (based on nominal concentrations). Slight inhibition of respiration rate of the sludge was recorded at 100 mg test substance per litre. The duplicate measurement confirmed the result of the first measurement (values of 12 and 18% inhibition only were recorded).
- Executive summary:
A study was conducted to determine the toxicity of the test substance on activated sludge microorganisms according to OECD Guideline 209, EU Method C.11 and ISO 8192, in compliance with GLP. Two 1 L test bottles were filled with 250 mL of test substance mixtures with initial loading rates of 200 mg/L. These mixtures were stirred in closed bottles for 26 h. Subsequently, 16 mL synthetic sewage feed, 200 mL sludge from a predominantly domestic sewage source and Milli-RO water up to 500 mL were added resulting in a final loading rate of 100 mg/L. This loading rate was tested in duplicate. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-h exposure period. Thereafter, oxygen consumption was measured and recorded for approximately 10 min. Slight inhibition of respiration rate of the sludge microorganisms was observed. The duplicate measurement confirmed the result of the first measurement (12 and 18% inhibition respectively). Therefore, no further testing was needed. Since all criteria for acceptability of the test were met, this study was considered to be valid. Under the study conditions, the EC50 of the test substance was determined to be greater than 100 mg/L (based on nominal concentrations) (Desmares-Koopmans, 2008).
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
A study was conducted to determine the toxicity of the test substance on activated sludge microorganisms according to OECD Guideline 209, EU Method C.11 and ISO 8192, in compliance with GLP. Two 1 L test bottles were filled with 250 mL of test substance mixtures with initial loading rates of 200 mg/L. These mixtures were stirred in closed bottles for 26 h. Subsequently, 16 mL synthetic sewage feed, 200 mL sludge from a predominantly domestic sewage source and Milli-RO water up to 500 mL were added resulting in a final loading rate of 100 mg/L. This loading rate was tested in duplicate. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-h exposure period. Thereafter, oxygen consumption was measured and recorded for approximately 10 min. Slight inhibition of respiration rate of the sludge microorganisms was observed. The duplicate measurement confirmed the result of the first measurement (12 and 18% inhibition respectively). Therefore, no further testing was needed. Since all criteria for acceptability of the test were met, this study was considered to be valid. Under the study conditions, the EC50 of the test substance was determined to be greater than 100 mg/L (based on nominal concentrations) (Desmares-Koopmans, 2008).
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