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EC number: 214-527-0 | CAS number: 1141-38-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 March 208 - 14 June 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July 22, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: 0012747069
- Expiration date of the lot/batch: January 24, 2019
- Purity test date: 99.96%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At ambient temperature
- Stability under test conditions: stable - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Flörsheim/Main, Germany
- Preparation of inoculum for exposure: The activated sludge was washed with reconstituted water. After that it was centrifuged, and the supernatant was decanted. The activated sludge pellet was re-suspended in washing medium. This treatment procedure was repeated twice.
- Pretreatment: Storing at ambient temperature
- Initial biomass concentration: The solids level in the microbial inoculum on testing day was 3.96 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.8–21.3 °C
- pH:
- 7.1–7.3
- Nominal and measured concentrations:
- Nominal: 10.0, 31.6, 100, 316 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL glass beakers, fill volume 500 mL
- Type: open (test vessels covered with watch glasses)
- Aeration: 0.5–1 L of air per hour
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per positive control (replicates): 4
- Volume of microbial inoculum: 200 mL
- Nitrification inhibitor used (delete if not applicable): N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was based on deionized water containing mineral salts. It was prepared within one month before use.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Inhibition of respiration
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Square root of 10
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- N-Allylthiourea
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 90.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 615 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid?
- Relevant effect levels: The EC50 of the total respireation inhibition of 3,5-dichlorophenot was 5.99 mg/L which is within the accepted range of 2-25 mg/L. The results of the reference substance are therefore considered to be valid. - Validity criteria fulfilled:
- yes
- Conclusions:
- The definitive test showed a concentration-response relationship with an inhibition of total respiration rate of up to 34.6% of control respiration at 1000 mg test item/L. Since even at the highest test concentration of 1000 mg/L the overall inhibition of total respiration was less than 50%, the EC50 is considered to be >1000 mg/L.
Reference
Description of key information
Since even at the highest test concentration of 1000 mg/L the overall inhibition of total respiration was less than 50%, the EC50 is considered to be > 1000 mg/L. The EC10 of 90.2 mg/L is therefore used as the key value for the risk assessment.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 90.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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