Tripotassium/trisodium 5-(4-chloro-6-(N-(4-(4-chloro-6-(5-hydroxy-2,7-disulfonato-6-(2-sulfonatophenylazo)-4-naphthylamino)-1,3,5-triazin-2-yl-(N-methyl)amino)phenyl)amino)-1,3,5-triazin-2-ylamino)-4-hydroxy-3-(2-sulfonatophenylazo)naphthalene-2,7-disulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From April 27, 1987 to April 29, 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Reference number: NBW7993/9
Physical state: Red purple powder (contains 9% moisture)
Purity: treated as 100%
Sample storage: dark and ambient temperature.

Analytical monitoring:

yes

Details on sampling:

The concentration of the test substance determined in the test solution at the start and finish of the test.

Vehicle:

yes

Remarks:

Dilution water

Details on test solutions:

1 liter solution was prepared by the addition of known weight of the test substance to dilution water (nominal 100 mg/L)

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organism: Daphnia magna
Medium: Reconstituted water medium
Temperature: 20±2˚C
Photoperiod: 16 h light 8 h dark
Age: <24 h old
Feeding: Daphnia not fed during the study

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

Total CaCO3: 162 mg/L

Test temperature:

20±1˚C

pH:

8.16 to 8.28

Dissolved oxygen:

9.0 to 9.2 mg/L

Nominal and measured concentrations:

Nominal: 100 mg/L
Measured concnetrations: at 0 h 94% of nominal and at 48 h 100% of nominal

Details on test conditions:

Borosilicate glass beakers were used as test vessels, with 4 replicates per exposure concentration, each containing 200 mL of test solution. When the test solutions were at test temperature 5 daphnia were added to each vessel total 20 daphnia per concentration.

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 97 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobilsed Daphnias were reported in the control and test group.

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 48 h EC50 of the substance in Daphnia magna was determined to be >97 mg a.i./L (mean measured).

Executive summary:

A study was conducted to determine the short-term toxicity of the substance to Daphnia magna according to OECD Guideline 202, in compliance with GLP. Four groups of 5 Daphnia magna each were exposed to a concentration of 100 mg/L for 48 h. Total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the study. No immobilised daphnids were reported in the control and test groups. Under the study conditions, the 48 h EC50 of the substance for Daphnia magna was determined to be >100 mg/L (nominal) and > 97 mg a.i./L (mean measured) (Brown, 1987).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

97 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the substance to Daphnia magnaaccording to OECD Guideline 202, in compliance with GLP. Four groups of 5 Daphnia magna each were exposed to a concentration of 100 mg/L for 48 h. Total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the study. No immobilised daphnids were reported in the control and test groups. Under the study conditions, the 48 h EC50 of the substance for Daphnia magna was determined to be >100 mgLL (nominal) and > 97 mg a.i./L (mean measured) (Brown, 1987).