Tin, dioctylbis(2,4-pentanedionato-.kappa.O2,.kappa.O4)-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Staatliches Gerwerbeaussichtsamt Hildesheim, Hildesheim, Germany

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

483-270-6

EC Name:

-

Cas Number:

54068-28-9

Molecular formula:

Hill formula: C26H48O4Sn CAS formula: C26H48O4Sn

IUPAC Name:

(3Z)-4-{[dioctyl({[(2Z)-4-oxopent-2-en-2-yl]oxy})stannyl]oxy}pent-3-en-2-one

Details on test material:

TIB KAT 223 VP10-054

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no statistically significant effect (p < 0.05) , ANOVA, Dunnett`s Method

Effect concentrations 2

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

318 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: P95=298 – 339 mg/L

Effect concentrations 3

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 ng/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 4

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

Two preliminary tests (non GLP) were carried out with three concentrations: 10 - 100 - 1000 mg/L.  Based on the results of the preliminary test the definitive study was carried out with 5 concentrations within the range 10 - 1000 mg/L in a geometrical series and with a dilution factor of 3.2 (5 replicates per concentration). Triplicates of the control without test item were included at the beginning and at the end of the test. A fresh sample of activated sludge taken from the sewage plantwas used for the test procedure. It was washed twice and adjusted with autoclaved tap water to a dry sludge concentration of 3.0 g/L ± 10 % (actual dry sludge concentration 3.85 g/L).The reference item was tested at three concentrations within the concentration range 58 - 180 mg/L and a dilution factor of 1.8 (triplicates per concentration).  The oxygen uptake rates of all samples was determined following an incubation period of 3 h

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC of the test item TIB KAT 223 is 100 mg/L.
The test item is not toxic at concentrations <= 318 mg/L
to activated sludge of a municipal sewage treatment plant.