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Diss Factsheets
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EC number: 452-810-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-11-10 to 2004-02-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: At the beginning of the test (duplicate samples from each testmedium and from the control) and after 48 and 96 hours (duplicate samples from each test medium with surviving fish. All samples were taken from the approximate center of the aquaria without mixing of the test media.
- Sample storage conditions before analysis: stored in a deep-freezer (~-20°C) until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the 2 highest concentrations were prepared by dissolving the exact amount of T002251 into 5 L of test water using ultrasonic treatment for 15 minutes and intense stirring for 90 minutes at room t°. Adequate volume of these two test media were diluted with test water to prepare the lower concentrations.
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: Zoohaus Schaub, CH-4410 Liestal, Switzerland)
- Length at study initiation (length definition, mean, range and SD): mean 4 cm, SD 0.2 cm
- Weight at study initiation (mean and range, SD): mean 0.58 g, SD 0.13 g
- Feeding during test: The fish were not fed during the test
- Food type: commercial dry fish food (Tetramin)
- Frequency: daily
ACCLIMATION
- Acclimation period: 1 week
- Acclimation conditions (same as test or not): same test water and temperature
- Type and amount of food: daily fed with commercial dry fish food
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 22.0 - 23.0 °C
- pH:
- 7.3 - 7.9
- Dissolved oxygen:
- 7.4-9.1 mg /L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- - Nominal concentrations: 1, 3.2, 10, 32, 100 mg/L.
- The analytically determined concentrations of the test item T002251 in the test medium were in the range from 87% to 101% of the nominal values. The results of the study can be based on the nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria
- Material, size, headspace, fill volume: capacity of 5 L
- Aeration: slightly aerated during the test period
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: less than 1.0 g/L
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Photoperiod: 16h light -to 8h dark photoperiod with a 30-minute transition period
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x3.2
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: Yes, the choice of the enlarged spacing factor was done according to the results of the range-finding test - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 10-32
- Details on results:
- - Behavioural abnormalities:
* 10 mg/L: after 24h and during the entire test, fish were mainly at the bottom of the aquarium.
- Mortality of control: 0
- The 96-h LC100= 32 mg/L. - Reported statistics and error estimates:
- Probit analysis or Moving average Interpolation could not be calculated due to the steep concentration-effect relationship.
LC50- values were determined as the geometric mean value of the 2 consecutive test concentrations with 0 and 100% mortality. - Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of of T002251 was investigated in a 96-h static fish test folllwing the OECD guideline 203. The 96-h LC50 was determined to be 18 mg T002251/L. The results of the test can be considered reliable without restriction.
Reference
Description of key information
The study of Seyfried (2004), investigating the acute toxicity of T002251 in Danio rerio according to OECD guideline 203, was considered as the key study for endpoint coverage. A LC50 = 18 mg/L was obtained after an exposure of 96 hours in a static test in freshwater. Based on the results it can be considered that T002251 is harmful to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 18 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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