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EC number: 605-076-4 | CAS number: 156928-09-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-04-18 to 2007-08-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- : Certificate issued by the Swiss GLP monitoring authorities.
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Using an analytical balance, 500.7 mg of the test substance was weighed and transferred to the designated test vessel with 284 mL tap water. The test substance was then dissolved by intense stirring for ten minutes at room temperature. After stirring, the appearance of the test medium was evaluated, and was found to be a clear solution. Then, 16 mL synthetic wastewater and 200 mL activated sludge inoculum were added. The inoculum had a sludge concentration of 2.4 g/L dry weight (corresponding to about 1.0 g dry material/L test medium). The sludge was added in time intervals of 15 minutes (an arbitrary but convenient interval) first to a control, secondly to the test solutions of the reference substance, thirdly to the test solution of the test substance, and finally to the second control.
- Controls: yes, concurrent no treatment
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: The study was performed with aerobic activated sludge from a wastewater treatment plant (ARA Ergolz 11, Fuellinsdorf, Switzerland) treating predominantly domestic wastewater.
- Laboratory culture: not applicable
- Method of cultivation: no data
- Preparation of inoculum for exposure: The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
- Pretreatment: no
- Initial biomass concentration: An aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material/L. During the holding period of two days prior to use, the sludge was fed daily with 50 mL synthetic wastewater/L (prepared according to guidelines) and was kept at room temperature under continuous aeration until use. Before use, the dry weight of the activated sludge was measured again in the inoculum used for the test. The pH of the activated sludge inoculum was 6.9. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- no data
- Test temperature:
- 20°C
- pH:
- 7.3-8.1
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass beakers, 2000 mL, 1500 mL, 500 mL
- Aeration: During the incubation period of 3 hours, the single test medium and the controls were continuously aerated by intense stirring on magnetic stirrers to avoid possible foaming and/or stripping of the test substance.
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): not applicable
- No. of organisms per vessel: no data
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH and dissolved oxygen concentrations were determined after the addition of synthetic wastewater and activated sludge in all test media and the controls at the start and at the end of the 3-hour incubation period. The water temperature was measured in one control at the start and at the end of the incubation period. Before the addition of activated sludge and synthetic wastewater, the appearance of the test media was recorded.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- For measurement of the respiration rate, a well-mixed sample of each test medium was poured into a BOD-flask after three hours incubation time, and was not further aerated. Then, the dissolved oxygen concentration was measured with an oxygen electrode and an oxygen meter and was continuously recorded. During measurement, the samples were continuously stirred on a magnetic stirrer. The oxygen consumption rate (in mg O2/L/min) was determined from the linear part of the respiration curve in the range 6.5-2.5 mg O2/L. In case of very rapid oxygen consumption, the range used was below the limits indicated above, but always within the linear part of the respiration curve. In case of low oxygen consumption, the rate was determined over a period of at least ten minutes.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: limit test
- Range finding study
- Test concentrations: not applicable
- Results used to determine the conditions for the definitive study: not applicable - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (98.8% by gas chromatography)
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no data
- Effect concentrations exceeding solubility of substance in test medium: No. In a pre-experiment the test substance was soluble at a concentration of at least 5 g/L in tap water. - Results with reference substance (positive control):
- - Results with reference substance valid?: The 3-hour EC50 is within the guideline-recommended range of 5-30 mg/L, confirming suitability of the activated sludge used.
- Relevant effect levels: The 3-hour EC50 of the reference substance 3,5-dichlorophenol was calculated to be 12 mg/L (the 95% confidence limits could not be calculated). - Reported statistics and error estimates:
- - The 3-hour EC50, EC20 and EC80 values of the test substance and their 95%-confidence limits could not be calculated because of the absence of a toxic effect.
- The 3-hour EC50 of the reference item 3,5-dichiorophenol was calculated by Probit Analysis.
- The NOEC is defined as the calculated concentration at which no significant toxic effect, i.e. < 15% inhibition, is determined (i.e. EC15). - Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge respiration inhibition test (according to OECD guideline 209) with activated sludge from a predominantly domestic sewage treatment plant resulted in a 3-h EC50 > 1000 mg/L. The results of the test can be consodered reliable without restriction.
Reference
The concentration of dissolved oxygen did not drop below 2.5 mg/L during the incubation period. Just before measurement of the respiration rates, the dissolved oxygen concentrations were at least 8.3 mg/L. The temperature in the test media, measured in one control, was 20 deg C at the start and at the end of the incubation period.
The oxygen consumption rates of the two controls (run at the start and at the end of the test) differed by 9% (guideline-recommended maximum variation: 15%).
Description of key information
The study of Bätscher (2007), investigating the toxicity of T002675 to microorganisms according to OECD guideline 209, was considered as the key study for endpoint coverage. The 3-h ELR50 was > 1000 mg/L. The 3-h NOELR was >= 1000 mg/L.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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