4-(tert-butyl)-N-sec-butyl-2,6-dinitroaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 123-2

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-(tert-butyl)-N-sec-butyl-2,6-dinitroaniline

EC Number:

251-607-4

EC Name:

4-(tert-butyl)-N-sec-butyl-2,6-dinitroaniline

Cas Number:

33629-47-9

Molecular formula:

C14H21N3O4

IUPAC Name:

N-sec-butyl-4-tert-butyl-2,6-dinitroaniline

impurity 1

Reference substance name:

Xylene

EC Number:

215-535-7

EC Name:

Xylene

Cas Number:

1330-20-7

Molecular formula:

C8H10

IUPAC Name:

m-xylene

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

5 d

Test conditions

Test temperature:

24-25°C

Nominal and measured concentrations:

Nominal test concentrations: 0.0050, 0.013, 0.032, 0.080, 0.20 and 0.50 mg a.i./L
Initial measured concentrations: 0.0044, 0.011, 0.029, 0.061, 0.12 and 0.19 mg a.i./L

Details on test conditions:

3700 to 4900 lux

Results and discussion

Effect concentrations

Key result

Duration:

5 d

Dose descriptor:

EC50

Effect conc.:

ca. 0.12 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Applicant's summary and conclusion

Executive summary:

Based on cell density, the 5 -day EC50 value was calculated to be 0.12 mg a.i./L (corresponding 95% confidence limit of 0.077 to 0.16 mg a.i./L)Since a statiscally significant effect on cell density was observed at all treatment levels tested, the EC05 value was calculated to provide the 5 -day NOEC value (US EPA, 1994). The 5 -day value for cell density (corresponding 95% confidence limits) was calculated to be 0.0053 mg a.i./L (0.0011 to 0.021 mg a.i./L). Based on these results and the criteria established by the US EPA, Butralin Technical would be classified as highly toxic to Selenastrum capricornutum.