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EC number: 268-369-2 | CAS number: 68082-25-7 This substance is identified by SDA Substance Name: C14-C18 and C16-C18 unsaturated alkyl carboxylic acid triethanol amine salt and SDA Reporting Number: 04-006-14.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch no.: 2016100027
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- acetone was used as solvent
- Details on test solutions:
- Acetone was used as solvent. A solvent control was run along with an untreated control. The maximum acetone concentration on test solutions was identical in all treatment (except untreated control) and did not exceed 0.01 % (v/v).
Stock solution A to E were prepared prior test start. Stock solution B to E were made diluting stock solution A.
1:10000 dilution of these stock solutions were used to result in the anticipated test item concentrations in test solution.
All stock solutions and their dilutions were prepared using acetone and in graduated flasks as follows:
- weighting 530 mg test item into a graduated flask, filling up to 10 mL (stock solution A = 53000 mg/L test item)
- stock solution B = 29404 mg/L test item: 2.774 mL stock solution A were filled to 5 mL
- stock solution C = 16358 mg/L test item: 1.543 mL stock solution A were filled to 5 mL
- stock solution D = 9084.2 mg/L test item: 0.857 mL stock solution A were filled to 5 mL
- stock solution E = 5045.6 mg/L test item: 0.476 mL stock solution A were filled to 5 mL
Test concentration: 0.50, 0.91, 1.64, 2.94, 5.30 mg/L test item nominal.
Test solutions were prepared directly in test vessels. After adding of 1 mL stock solution, test solutions were stirred thoroughly. Each test vessel contained a total volume of 10 L of test solution. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- rainbow trout - Oncorhynchus mykiss
mean length: 5.00 +/- 0.19 cm
mean weight: 1.09 +/- 0.17 g
mean loading: 0.76 g fish/L
Time for acclimatisation: 55 days
Daily light-dark-cycle: 16 hours light:8 hours dark
Oxygen concentration: at least 80 % of air saturation value
Temperature: 15.0 °C
Feeding: daily (fish food), until 24 hours before test start no feeding during the test
During the acclimatisation period, fish were not exposed to the test item. Mortality of fish during the acclimatisation period was 0.00 %. 7 days before test start, fish were transferred into fresh test medium. Equal environmental conditions as used during the test were given. - Test type:
- static
- Water media type:
- other: reconstituted water according to Guideline 203
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- Water hardness: 10 - 250 mg CaCO3/L
water is aerated until oxygen saturation is achieved and then stored for about two days without further aeration before use - Test temperature:
- 14.3 – 15.5°C, recorded continuously
- pH:
- pH of the test medium before start: 7.58
pH values of test solutions: 7.44 - 7.88 - Dissolved oxygen:
- Oxygen concentration: 8.45 to 8.95 mg/L O2, corresponding to 86.4 - 91.5 % saturation of the maximum saturation.
Test medium was aerated until oxygen saturation was achieved, then stored for quarantine days without aeration before use. Permanent aeration was employed during the test. - Nominal and measured concentrations:
- Test concentration: 0.50, 0.91, 1.64, 2.94, 5.30 mg/L test item nominal
- Details on test conditions:
- Test vessel (pool): stainless steel container (approx. 13 L volume)
Test volume : 10 L
Number of fish/vessel (= replicate): expsoure of 7 fish/replicate
Number of replicates/treatment: 1
Loading: maximum loading of 1.0 g fish/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: water solubility = 5.3 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: water solubility = 5.3 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: water solubility = 5.3 mg/L
- Details on results:
- Under given conditions, Triethanolamine trioleate did cause mortality exclusively at the highest employed test concentration of 5.30 mg/L test item nominal. An effect of 28.57 % mortality at test end was observed. Significant effects were determined as LOEC > 5.30 mg/L test item nominal. The corresponding NOEC was ≥ 5.30 mg/L test item nominal.
- Reported statistics and error estimates:
- The determination of lowest observed effects concentration (LOEC) was carried out by hypothesis testing using Fisher`s Exact Binomial Test (p ≤ 0.05, one-sided greater) for binomial distributed data. Lethal concentrations LCx were determined by concentration-response modelling (Probit analysis, maximum likelihood). Statistical evaluation was carried out using ToxRat Professional 3.2.1 (RATTE, 2015).
The statistical test determined a LOEC higher than the highest employed test item concentration. Different settings at the statistical software could not improve this situation. For effect concentration estimates LCx, confidence intervals could only be partially be determined. The LC50 was determined outside the range of applied concentrations (LC50 = 5.71 mg/L test item nominal). - Sublethal observations / clinical signs:
Observations
Test item (mg/L)
Test item (mg/L)
Test item (mg/L)
Test item (mg/L)
Test item (mg/L)
Test item (mg/L)control 2
0.50
0.91
1.64
2.94
5.30
Time after application
mortality (%)
mortality (%)
mortality (%)
mortality (%)
mortality (%)mortality (%)
3 h
0.00
0.00
0.00
0.00
0.00
0.00
6 h
0.00
0.00
0.00
0.00
0.00
0.00
24 h
0.00
0.00
0.00
0.00
0.00
0.00
48 h
0.00
0.00
0.00
0.00
0.00
14.29
72 h
0.00
0.00
0.00
0.00
0.00
28.57
96 h
0.00
0.00
0.00
0.00
0.00
28.57
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the OECD 203 test, the test item caused mortality exclusively at the highest employed test concentration of 5.30 mg/L test item nominal (maximun water solubility). An effect of 28.57 % mortality at test end was observed. Significant effects were determined as LOEC > 5.30 mg/L test item nominal. The corresponding NOEC was ≥ 5.30 mg/L test item nominal.
Reference
Description of key information
The test item caused mortality exclusively at the highest employed test concentration of 5.30 mg/L test item nominal (maximun water solubility). An effect of 28.57 % mortality at test end was observed.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.3 mg/L
Additional information
LC50 > 5.3 mg/L/96h. Solubility of the substance: 5.3 mg/L
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