Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated

Endpoint summary

Description of key information

Hydrolysis: A hydrolysis trail was conducted to investigate the feasibility of using pH measurement to assess hydrolytic degradation of the test substance. Due to the complex nature of the UVCB substance, a copper surrogate ICP methodology was previously developed and implemented for the determination of the solubility of the test item. However, for a hydrolysis study this copper surrogate methodology, where all copper containing components were collectively measured as an integrated total copper response, would not be capable of distinguishing between parent compound and components and hydrolytic degradation products. Therefore, it was determine that pH measurement and changes in pH was a possible potential measure of possible parent degradation. Several pH values were used during sampling days 1, 2, 3, and 6. The pH values did not show any apparent patterns or trends. Based on these values, pH was deemed not a suitable indicator of hydrolytic degradation of the test substance.

 

Biodegradation: The test substance was tested for the ready biological degradability in Closed Bottle Test performed according to: Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008. The results of biological degradation are related to experimentally determined COD values of test and reference substance at the beginning of the test. The test substance was sufficiently soluble in used mineral medium so the dosage from the stock solution was carried out. Sodium benzoate was used as the reference substance. The dosage was carried out from the stock solution.

COD of the test substance in medium at the beginning of the main test: 3.40 mg·L-1.

COD of reference substance in medium at the beginning of the main test: 3.45 mg·L-1.

In parallel to the main test the toxicity test was performed. Based on the test substance contains nitrogen in the chemical composition, the oxidized nitrogen forms were determined and the correction for nitrification was carried out. The test was performed at temperature of 20 ± 1 °C with the pH values of solutions 7-8 at the beginning of the test. The prescribed validity criteria in the test were fulfilled. The test substance was not inhibiting for the used inoculum. In this 28-day study of ready biological degradability the degradation of 7.1 % of the test substance was attained in the end of study.  

Adsorption: The study was performed to estimate the adsorption coefficient of the test substance in soil and sludge using a high performance liquid chromatography (HPLC) based methodology, per OECD Guideline 121, estimation of the adsorption coefficient on soil and sewage sludge using HPLC. Under the chromatographic conditions specified, the test substance eluted as 3 peaks on the ultraviolet (UV) detector. The corresponding Log Koc for the test substance ranged from unretained (<Urea) to 1.08.