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EC number: 701-407-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test system Micro-organisms in activated sludge.
Source Municipal sewage treatment plant: 'Waterschap Aa en Maas',
's- Hertogenbosch, The Netherlands, receiving predominantly
domestic sewage.
Preparation of the sludge The sludge was coarsely sieved (1 mm) and allowed to settle.
The supernatant was removed and ISO-medium was added. A
small amount of the sludge was weighed and dried overnight at
ca. 105°C to determine the amount of suspended solids (3.0 g/L
of sludge, as used for the test). The pH was 7.8 on the day of
testing. The batch of sludge was used one day after collection;
therefore, 50 mL of synthetic medium was added per litre of
activated sludge at the end of the collection day. The sludge
was kept aerated at test temperature until use. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Adjusted ISO-medium, formulated using RO water (tap water
purified by reverse osmosis; GEON Waterbehandeling, Berkel-
Enschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
Performance of the test1 The synthetic medium (16 mL) made up to 50 mL with
Milli- RO and 200 mL test item solution were mixed (total
volume 250 mL). The pH was determined. Thereafter 250 mL
activated sludge was added. This was the start of the test.
After the 3-hour contact time, the oxygen consumption was
recorded for a period of approximately 10 minutes. During
measurement, the sample was not aerated but continuously
stirred on a magnetic stirrer.
The pH was determined in the remaining part of the reaction
mixture. This procedure was repeated for all test/reference item
concentrations and controls.
The medium temperature was recorded continuously in
temperature control vessel. The temperature control vessel were
identically prepared compared to the control vessels. A
temperature control vessel with a REES sensor was placed in
each fume cupboard of the climate room.
Oxygen recording Determination of oxygen was performed with multiple oxygen
probes connected to a BlueBox (GO-Systemelektronik GmbH,
Germany), a multichannel measuring and controlling system. - Test temperature:
- 20-22 C
- Details on test conditions:
- Contact time 3 hours, during which aeration and stirring took place.
Vessels All glass open bottles/vessels.
Milli-RO / Milli-RO water Tap water purified by reverse osmosis (Millipore Corp.,
Bedford, Mass., USA).
Synthetic medium 16 g peptone
(=sewage feed) 11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
Dissolved in Milli-RO water, made up to 1 litre and filtered.
The pH was within 7.5 ± 0.5.
Air supply Clean, oil-free air.
Aeration The aeration was adjusted in such a way that the dissolved
oxygen concentration at the start was above 60-70% saturation
(60% of air saturation is > 5 mg/L at 20°C) and to maintain the
sludge flocs in suspension. - Reference substance (positive control):
- yes
- Duration:
- 3 h
- Dose descriptor:
- other: NOELR
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: ELR10
- Effect conc.:
- 7.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: ELR20
- Effect conc.:
- 21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: ELR50
- Effect conc.:
- 149 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- Combined limit/range-finding test
The combined limit/range-finding test showed 17, 62 and 84% inhibition of the respiration
rate at a loading rate of 10, 100 and 1000 mg/L, respectively. Therefore, the expected ELR50
was between a loading rate of 10 and 100 mg/L. There was no oxygen uptake from abiotic
processes.
All test conditions and acceptability criteria prescribed by the study plan were met.
Final test
Inhibition of the respiration rate
The effects observed were in agreement with what was expected based on the results of the
combined limit/range-finding test. The results of this test allowed for reliable determination
of a NOELR and ELRx values.
No statistically significant inhibition of the respiration rate of the sludge was recorded at or
below a loading rate of 3.2 mg PACM BADGE adduct per litre
At higher loading rates the inhibitory effect of PACM BADGE
adduct on aerobic waste water (activated sludge) bacteria increased with increasing loading
rate, ranging from 16% inhibition at 10 mg/L to 77% at 1000 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test PACM BADGE adduct was not toxic to waste water bacteria (activated sludge) at or below a loading rate of 3.2 mg/L (3-hour NOELR).
The 3-hour ELR10 was at a loading rate of 7.3 mg/L (95% confidence interval: 1.7 – 16 mg/L).
The 3-hour ELR20 was at a loading rate of 21 mg/L (95% confidence interval: 7.5 - 37 mg/L).
The 3-hour ELR50 was at a loading rate of 149 mg/L (95% confidence interval: 93 – 254 mg/L). - Executive summary:
Under the conditions of this present test PACM BADGE adduct was not toxic to waste water bacteria (activated sludge) at or below a loading rate of 3.2 mg/L (3-hour NOELR).
The 3-hour ELR10 was at a loading rate of 7.3 mg/L (95% confidence interval: 1.7 – 16 mg/L).
The 3-hour ELR20 was at a loading rate of 21 mg/L (95% confidence interval: 7.5 - 37 mg/L).
The 3-hour ELR50 was at a loading rate of 149 mg/L (95% confidence interval: 93 – 254 mg/L).
Reference
Description of key information
Under the conditions of this present test PACM BADGE adduct was not toxic to waste water bacteria (activated sludge) at or below a loading rate of 3.2 mg/L (3-hour NOELR).
The 3-hour ELR10 was at a loading rate of 7.3 mg/L (95% confidence interval: 1.7 – 16 mg/L).
The 3-hour ELR20 was at a loading rate of 21 mg/L (95% confidence interval: 7.5 - 37 mg/L).
The 3-hour ELR50 was at a loading rate of 149 mg/L (95% confidence interval: 93 – 254 mg/L).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 149 mg/L
Additional information
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