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EC number: 248-957-5 | CAS number: 28313-51-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Nov 1982 - Jan 1983
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- [2-[ethyl[3-methyl-4-[(3-phenyl-1,2,4-thiadiazol-5-yl)azo]phenyl]amino]ethyl]trimethylammonium methyl sulphate
- EC Number:
- 248-957-5
- EC Name:
- [2-[ethyl[3-methyl-4-[(3-phenyl-1,2,4-thiadiazol-5-yl)azo]phenyl]amino]ethyl]trimethylammonium methyl sulphate
- Cas Number:
- 28313-51-1
- Molecular formula:
- C22H29N6S.CH3O4S
- IUPAC Name:
- {2-[ethyl({3-methyl-4-[(E)-2-(3-phenyl-1,2,4-thiadiazol-5-yl)diazen-1-yl]phenyl})amino]ethyl}trimethylazanium methyl sulfate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Basic Red 23 Methyl sulfate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- Bor: WISW (SPF Cpb)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen
- Age at study initiation: male: 9 weeks; female: 14 weeks
- Weight at study initiation: male: 164 - 200g; female: 158 - 186g
- Fasting period before study: 16h before until 4h after application
- Housing: 5 animals/ cage (makrolon typ III)
- Diet (e.g. ad libitum): Altromin R 1324, ad libitum
- Water (e.g. ad libitum): mains water, ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5°C
- Humidity (%): 60 +/- 5%
- Photoperiod (hrs dark / hrs light): 12h/12h
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 20mL/kg bw
- Doses:
- 0.31, 1.00, 1.25, 1.60 and 5.0 g/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: day of application: multiple observations; during 14 days observation: twice per working day, once on weekend and holiday; weekly weighing
- Necropsy of survivors performed: yes (randomly)
- Other examinations performed: clinical signs, body weight - Statistics:
- calculation of LD50 with CI for p<0.05 according to Rosiello et al. J. Tox. Environ. Health, 1977(3):797
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 200 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 100 - <= 1 500
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 008 mg/kg bw
- Based on:
- act. ingr.
- Mortality:
- animals died with in 6 days after application
- Clinical signs:
- 1.0 to 5.0 g/kg bw: bad general condition, sedation and growth delay
additionally for male animals from 1.25g/kg bw and for female animals from 1.0 g/kg bw: abdominal and lateral position
symptoms occurred 1 hour after application and disappeared at the latest after 8 days
no clinical signs in the lowest dose of 0.31 g/kg bw - Body weight:
- retarded growth between 1 and 5 g/kg bw
- Gross pathology:
- in died animals of doses 1.0 to 5.0 g/kg bw: stomach with partly massive mucosal bleeding
Any other information on results incl. tables
Results for male and female animals
dose [g/kg bw) |
toxicological results | duration of symptoms | time of death | mortality [%] |
||
died animals | animals with symptoms | treated animals | ||||
0.31 | 0 | 0 | 10 | - | - | 0 |
1.00 | 1 | 10 | 10 | 1h - 8d | 3d | 10 |
1.25 | 4 | 10 | 10 | 1h - 8d | 5d - 6d | 40 |
1.60 | 9 | 10 | 10 | 1h - 8d | 2d - 6d | 90 |
5.00 | 10 | 10 | 10 | 1h - 3d | 24h - 3d | 100 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The LD50 of the test substance for acute oral toxicity to female rats was calculated to be 1200 mg/kg bw.
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