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EC number: 276-470-8 | CAS number: 72208-20-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 January to 05 March 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 2-[2-[4-[(2-cyanoethyl)ethylamino]-o-tolyl]vinyl]-1,3,3-trimethyl-3H-indolium dihydrogen phosphate
- EC Number:
- 276-470-8
- EC Name:
- 2-[2-[4-[(2-cyanoethyl)ethylamino]-o-tolyl]vinyl]-1,3,3-trimethyl-3H-indolium dihydrogen phosphate
- Cas Number:
- 72208-20-9
- Molecular formula:
- C25H30N3.H2O4P C25H32N3O4P
- IUPAC Name:
- 2-[2-[4-[(2-cyanoethyl)ethylamino]-o-tolyl]vinyl]-1,3,3-trimethyl-3H-indolium dihydrogen phosphate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan Winklemann, Borchen, Germany
- Age at study initiation: Males 6 -7 weeks, Females 8 - 9 weeks
- Weight at study initiation: Initial mean weight: Males 196 g, Females 190 g
- Fasting period before study: 16 hours prior
- Housing: Conventational conditions - Makrolon Typo-III cages, 5 to a cage
- Diet (e.g. ad libitum): Altromin 1324 pellets, ad libitum
- Water (e.g. ad libitum): Tap water, ad libitum
- Acclimation period: At least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-2
- Humidity (%): 55 +/- 5
- Air changes (per hr): 15 - 20 per hour
- Photoperiod (hrs dark / hrs light): 12 hour light/dark (artifical light from 6am to 6pm)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- The test substance was formulated in tap water at room temperature under stirring on a magnetic stirrer. The formulation was intragastrically applied by gavage to 3 males and 3 females per dose (2000 mg/kg only females) at a constant application volume of 10 ml/kg body weight. The test substance was formulated in the application vehicle immediately prior to treatment.
- Doses:
- females: 200, 500, 2000 mg/kg body weight
males: 200, 500 mg/kg body weight - No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Several times on day of administration, twice daily during the observation period (once on weekends & bank holidays)
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 500 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks:
- ca. 100% purity
- Mortality:
- All females died between 2 hours and 2 days after gavage of 2000 mg/kg bw. At 200 and 500 mg/kg bw no deaths were observed in male and female rats.
- Clinical signs:
- After administration of 2000 mg/kg body weight the following clinical signs were observed in female rats: apathy, piloerection, poor general condition and hunched posture.
A single dose of 200 mg/kg and 500 mg/kg was tolerated by male and female rats without any clinical signs of toxicity. - Body weight:
- Body weight development of male and female rats was not noticeable affected in this dosages.
- Gross pathology:
- Reddened mucosae of the stomach and intestine as well as reddenig of the subcutaneous fatty tissue were observed at necropsy in the intercurrent death animals. None of the animals sacrificed at the end of study showed any noticeable gross pathological findings.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The LD50 was estimated to be between 500 and 2000 mg/kg body weight for male and female rats.
- Executive summary:
Acute toxicological investigations of male and female Wistar rats were conducted after single oral administration of BASIC RED 015 FL. 200%. After administration of 2000 mg/kg body weight the following clinical signs were observed in female rats: apathy, piloerection, poor general condition and hunched posture.
A single dose of 200 mg/kg and 500 mg/kg was tolerated by male and female rats without any clinical signs of toxicity. Body weight development of male and female rats was not noticeable affected in this dosages.
All females died between 2 hours and 2 days after gavage of 2000 mg/kg bw. At 200 and 500 mg/kg bw no deaths were observed in male and female rats.
Reddened mucosae of the stomach and intestine as well as reddenig of the subcutaneous fatty tissue were observed at necropsy in the intercurrent death animals. None of the animals sacrificed at the end of study showed any noticeable gross pathological findings.
The LD50 was estimated to be between 500 and 2000 mg/kg body weight for male and female rats.
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