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EC number: 208-634-1 | CAS number: 536-45-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 - 31 Aug 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Aerobic activated sludge from sewage treatment plant Bensheim, Germany
- Storage conditions: aerated
- Storage length: aerated for 2 days
- Preparation of inoculum for exposure: Activated sludge was washed by centrifugation and the supernatant liquid phase was decanted; solid material was resuspended in test water and centrifuged again and repeated three times; based on the the ration of wet sludge to its dry weight calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated for 2 days.
- Pretreatment: An aliquot of sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
- Concentration of sludge: 3.5 g dry material per litre - Duration of test (contact time):
- 28 d
- Initial conc.:
- 102.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 160.4 mg/L
- Based on:
- ThOD
- Remarks:
- ThODNH4
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according the OECD guideline 301
- Test temperature: 22 ± 1 °C
- pH: 7.5 to 8.2
- Continuous darkness: yes
- Other: continuous stirring
TEST SYSTEM
- Culturing apparatus and measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany (Manometric Test System, 500 mL fill volume in flasks)
- Number of culture flasks/concentration: 2
- Details of trap for CO2: potassium hydroxide solution (45%)
SAMPLING
- Sampling method: change of pressure in the test flasks was measured by means of a manometric method
CONTROL AND BLANK SYSTEM
- Inoculum control: 2 replicates
- Abiotic sterile control: 1 replicate (poisoned with HgCl2)
- Toxicity control: 1 replicate
- Other: procedure control - 1 replicate - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 89
- Sampling time:
- 28 d
- Details on results:
- Test item is not inhibitory to aerobic activated sludge microorganisms since degradation was >25% within 14 days (30% after 14 d and 46% after 28 d).
- Results with reference substance:
- The reference item was sufficiently degraded to 76% after 14 days and to 79% after 28 days of incubation.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further information see section "Any other information on results incl tables".
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Test item is readily biodegradable and not inhibitory to aerobic activated sludge microorganisms since degradation was >25% within 14 days (30% after 14 d and 46% after 28 d).
Reference
Table 1: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
8% |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
>60% |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
12.5 mg/L |
yes |
Description of key information
Readily biodegradable: 89% within 28 d (OECD 301F)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
One GLP guideline study according to OECD 301F is available investigating the ready biodegradability of sodium anisate (CAS 536 -45 -8). A mixed microbial population of the activated sludge from sewage treatment plant Bensheim, Germany was inoculated with a nominal concentration of 102.7 mg/L test substance and incubated for 28 d under controlled conditions in the dark. Degradation was followed by the determination of dissolved oxygen content each day. An inoculum blank, a reference control and a toxicity control were run in parallel. The derived 28 d degradation rate was 89% for the test substance and 30% for the toxicity control after 14 d. Since the test substance reached the pass level of 60% biodegradation within 28 d it is considered to be readily biodegradable according to the OECD criteria. Due to the fact that the degradation rate of the toxicity control was >25% after 14 d, toxic effects of the test substance to the inoculum can be excluded.
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