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EC number: 277-633-6 | CAS number: 73912-21-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-11-17 - 1997-11-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted under GLP according to EU method C.1 on the registered substance itself without analytical monitoring due to the substances intrinsic properties, i.e. poor water solubility. The present proceedings correspond to the preparation of a WAF, which is in line with the recommendations in ECHA's guidance R.7b.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Council Directive 92/69/EEC
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing
- Controls: concurrent no treatment, synthetic fresh water in accordance with ISO
- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: Brachydanio rerio HAMILTON BUCHANAN
- Source: Bio International B.V. (Netherlands)
- Age at study initiation (mean and range, SD): ca. 6 months
- Length at study initiation (length definition, mean, range and SD): 2.5 - 3.2 cm
- Weight at study initiation (mean and range, SD): 1.93 - 2.10 g (10 fishes)
ACCLIMATION
- Acclimation period: 4 months
- Health during acclimation (any mortality observed): <2% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 14.1°dH
- Test temperature:
- 21.4°C - 21.8°C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- 8.0 - 8.9
- Nominal and measured concentrations:
- 0; 1 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: test vessels (300 x 135 x 200 mm), 5L test medium
- Aeration: ventilated
- No. of organisms: 10 per concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water in accordance with ISO.
OTHER TEST CONDITIONS
- Photoperiod: 16h light, 8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : visible effects on appearance and behaviour of fish, mortality, daily intervals
TEST CONCENTRATIONS
- Test concentrations: 0; 1 mg/L (nominal) - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- - Behavioural abnormalities: 10 animals showed normal swimming action at all doses and observation times
- Observations on body length and weight: no relevant deviations from control noted
- Mortality of control: 0%
- Other adverse effects control: none noted
- Abnormal responses: none noted
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: yes, undissolved particles found
- Effect concentrations exceeding solubility of substance in test medium: 1 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted under GLP according to EU method C.1 on the registered substance itself without analytical monitoring due to the substances intrinsic properties, i.e. poor water solubility. The present proceedings correspond to the preparation of a WAF, which is in line with the recommendations in ECHA's guidance R.7b. The method is hence to be considered scientifically reasonable. Hence, the results can be considered as reliable to assess the effects of 12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl- to aquatic organisms, here, zebra fish. Taking into account the determined effect values after 96h, based on nominal concentrations, i.e. LC50, LC0, EC50, EC0 = resp. ≥ 1 mg/L, based on mortality resp. swimming behaviour, the substance does not need to be classified as aquatic acute cat. 1 according to Regulation (EC) No. 1272/2008. Since 1 mg/L (nominal) was the only concentration tested, a classification as aquatic chronic cat. 2 or lower can not be categorically excluded. However, this concentration resulted in precipitation, i.e. it is above the water solubility of the substance. Based on the procedure as described in the test report, the test solutions need to be considered as a WAF. Hence, it can be assumed that the observed effects, i.e. actually the lacking of mortality or behavioural abnormalities, was observed at concentrations maximally as high as the substance water solubility and the actual LC50 would virtually be above the water solubility of the substance. In this case, referring to the actual concentration, the safety net classification as aquatic chronic cat. 4 would be triggered.
- Executive summary:
In a 96-h acute toxicity study, zebra fish (Brachydanio rerio HAMILTON BUCHANAN) were exposed to12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl-at nominal concentrations of 0 (control), and 1 mg/L under static conditions. The 96-h LC50was >1 mg/L. The EC50,EC0and LC0values, based on mortality or swimming behaviour, were > resp. ≥ 1 mg/L. Based on the results of this study,12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl-would not be classified as aquatic acute cat. 1 to zebra fish in accordance with Regulation (EC) No. 1272/2008. Since the nominal concentration of 1 mg/L is above the water solubility of the test substance, classification as aquatic chronic cat. 4 would be triggered.
This toxicity study is classified as acceptable and satisfies the guideline requirement for an acute toxicity study in fish.
Reference
Description of key information
Short-term toxicity to fish: LC50(96h) > 1mg/l, EC0(96h) >= 1mg/l (nominal) for Danio rerio, static, direct weighing of 1 mg/l (EU method C.1, GLP)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 mg/L
Additional information
Nominal concentration is above the water solubility of the test item: Its estimated water solubility is 1.171 E-5 µg/l (no testing could be performed) and the test item needs to be considered as insoluble.
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