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EC number: 607-854-9 | CAS number: 2605-78-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 20th, 2007 - July 7th, 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Analytical purity: 82.3%
- Purity test date: Not available
- Lot/batch No.: GN-8
- Expiration date of the lot/batch: Not available
- Appearance: Amber coloured, extremely viscous liquid
- Storage: In the dark at room temperature
- Additional information on test material used for the study: It was attempted to purify the substance to a purity of >80% by using an alternative synthetic route, as opposed to the usual commercial route of synthesis which results in a purity of the substance of approximately 40% in water. This alternative synthetic route generated the substance at 82.3% purity, however it also generated an impurity (methanol at 4.0%) which is not present in the commercial substance. Based on the classification and labelling of methanol, it is considered that this additional impurity would not have an influence on any of the endpoints discussed in this dossier other than the acute oral toxicity study. Therefore all studies (including the one covering this endpoint) contained in this dossier (other than the acute oral toxicity study) were conducted on the 82.3% pure substance containing the 4.0% methanol impurity. The acute oral toxicity study has been conducted on the commercially generated substance at a purity of approximately 40% in water. - Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken from the control (replicates R1-R6 pooled) and each test group (replicates R1-R3 pooled) at 0 and 72 hours for quantitative analysis. Duplicates samples were taken and stored at approximately -20°C for further analysis if necessary.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dissolved directly in culture medium
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: CCAP 276/20
- Source : Culture Collection of Algae and Protozoa (CCAP), Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland
- Age of inoculum (at test initiation):
- Method of cultivation: Master cultureS maintained in the laboratory under constant aeration and constant illumination at 21 ± 1°C - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Hardness:
- Not reported
- Test temperature:
- 24 ± 1°C
- pH:
- 7.3-7.8
- Dissolved oxygen:
- Not reported
- Nominal and measured concentrations:
- Definitive test: control, 1, 3.2, 10, 32, 100 mg a.i./L (analytically confirmed nominal concentrations)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass conical flask (250 mL)
- Type : Closed (vessels covered to reduce evaporation)
- Fill volume: 100 mL
- Aeration: No aeration
- Initial cells density: 4E3 cells/mL
- Mean cell density of control at 0 hours: 4.56E3 cells/mL
- Mean cell density of control at 72 hours: 4.22E5 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: No
- Detailed composition if non-standard medium was used:
**NaNO3 25.5mg/l
**MgCl2.6H2O 12.164mg/l
**CaCl2.2H2O 4.41mg/l
**MgSO4.7H2O 14.7mg/l
**K2HPO4 1.044mg/l
**NaHCO3 15.0mg/l
**H3BO3 0.1855mg/l
**MnCl2.4H2O 0.415mg/l
**ZnCl2 0.00327 mg/l
**FeCl3.6H2O 0.159 mg/l
**CoCl2.2H2O 0.000012mg/l
**Na2EDTA.2H2O 0.30 mg/l
**Na2SeO3.5H2O 0.30 mg/l
The culture medium was prepared using reverse osmosis purified deionised water and the pH adjusted to 7.5 ± 0.1 with 0.1N NaOH or HCL.
OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Photoperiod: Continuous illumination
- Light intensity and quality: 7000 lux, warm white lighting (380-730 nm)
- Temperature: 24 ±1 °C
- Agitation: Constant shaking by orbital shaker (150 rpm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cell density determined using a Coulter Multisizer Particle Counter.
TEST CONCENTRATIONS
- Range-finding test(s): Yes (one)
- Test concentrations (range-finding test): 0.1, 1.0, 10 and 100 mg a.i./L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Basis for effect:
- cell number
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 23 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Basis for effect:
- cell number
- Remarks:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 1-octanamine, N, N-dimethyl-, N-oxide
- Details on results:
- All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control or test culture at 72 hours.
At the start of the test all control and test cultures were observed to be clear colourless solutions. After the 72-hour test period all control, 1, 3.2 and 10 mg/L test cultures were observed to be pale green dispersions. The 32 mg/L test cultures were observed to be very pale green dispersions whilst the 100 mg/L test cultures were observed to be clear colourless solutions. - Results with reference substance (positive control):
- The results from the positive control with potassium dichromate were within the normal range for this reference material. The 72-hour EC50 value calculated was 0.49 mg/L based on growth rate, 0.23 mg/L based on biomass, and 0.22 mg/L based on yield. The 72-hour NOEC value calculated was 0.125 mg/L based on growth rate, 0.0625 mg/L based on biomass, and 0.0625 mg/L based on yield.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72-hour 50% effective concentration ErC50 of 49 mg a.i./L (1-octanamine, N, N-dimethyl-, N-oxide) and a 10% effective concentration ErC10 of 10 mg a.i./L were obtained for growth rate inhibition based on analytically confirmed nominal concentrations of the test material.
- Executive summary:
The toxicity of the test material to freshwater green algae (Desmodesmus subspicatus) was determined in a GLP-compliant study performed in accordance with OED Guideline 201 and EU Method C.3.
A 72-hour 50% effective concentration ErC50 of 49 mg a.i./L (1-octanamine, N, N-dimethyl-, N-oxide) and a 10% effective concentration ErC10 of 10 mg a.i./L were obtained for growth rate inhibition based on analytically confirmed nominal concentrations of the test material.
Reference
Daily specific growth rates for controls
Daily Specific Growth Rate |
||||
Day 0 – 1 |
Day 1 - 2 |
Day 2 – 3 |
||
Control |
R1 R2 R3 R4 R5 R6 Mean |
0.058 0.067 0.059 0.068 0.061 0.061 0.062 |
0.062 0.055 0.059 0.062 0.072 0.064 0.062 |
0.076 0.082 0.067 0.061 0.063 0.065 0.069 |
Inhibition of growth rate, yield and biomass
Nominal Concentration (mg/l notifiable substance) |
Growth Rate (cells/ml/hour) |
Yield (cells/ml) |
Biomass Integral |
||||
0-72h |
% Inhibition |
0-72h |
% Inhibition* |
0-72h |
% Inhibition |
||
Control |
R1 R2 R3 R4 R5 R6 Mean SD |
0.065 0.068 0.062 0.064 0.065 0.063 0.065 0.002 |
- |
4.34E+05 5.33E+05 3.33E+05 3.89E+05 4.34E+05 3.80E+05 4.17E+05 6.85E+04 |
- |
7.12E+06 8.48E+06 5.84E+06 7.14E+06 7.75E+06 6.73E+06 7.18E+06 8.95E+05 |
- |
1.0 |
R1 R2 R3 Mean SD |
0.062 0.069 0.062 0.064 0.004 |
5 [6] 5 1 |
3.37E+05 5.85E+05 3.32E+05 4.18E+05 1.45E+05 |
0 |
6.06E+06 8.73E+06 6.17E+06 6.98E+06 1.51E+06 |
16 [22] 14 3 |
3.2 |
R1 R2 R3 Mean SD |
0.061 0.065 0.065 0.064 0.002 |
6 0 0 2 |
3.29E+05 4.33E+05 4.34E+05 3.99E+05 6.06E+04 |
4 |
5.74+06 6.91E+06 6.74E+06 6.47E+06 6.31E+06 |
20 4 6 10 |
10 |
R1 R2 R3 Mean SD |
0.064 0.068 0.063 0.065 0.003 |
2 [5] 3 0 |
3.86E+05 5.31E+05 3.62E+05 4.26E+05 9.16E+04 |
[2] |
6.32E+06 8.05E+06 5.68E+06 6.68E+06 1.23E+06 |
12 [12] 21 7 |
32 |
R1 R2 R3 Mean SD |
0.037 0.037 0.037 0.037 0.000 |
43 43 43 43 |
5.42E+04 5.27E+04 5.14E+04 5.28E+04 1.41E+03 |
87 |
9.79E+05 9.63E+05 1.03E+06 9.92E+05 3.71E+04 |
86 87 86 86 |
100 |
R1 R2 R3 Mean SD |
0.020 0.021 0.020 0.020 0.001 |
69 68 69 69 |
1.22E+04 1.35E+04 1.22E+04 1.26E+04 7.41E+02 |
97 |
2.49E+05 2.11E+05 1.90E+05 2.17E+05 3.02E+04 |
97 97 97 97 |
*In accordance with the OECD test guideline only the mean value for yield for each test concentration is calculated
R1- R6= Replicates 1-6
SD = Standard Deviation
Description of key information
The toxicity of the test material to freshwater green algae (Desmodesmus subspicatus) was determined in a GLP-compliant study performed in accordance with OED Guideline 201 and EU Method C.3.
A 72-hour 50% effective concentration ErC50 of 49 mg a.i./L (1-octanamine, N, N-dimethyl-, N-oxide) and a 10% effective concentration ErC10 of 10 mg a.i./L were obtained for growth rate inhibition based on analytically confirmed nominal concentrations of the test material.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 49 mg/L
- EC10 or NOEC for freshwater algae:
- 10 mg/L
Additional information
The toxicity of the test material to freshwater green algae (Desmodesmus subspicatus) was determined in a GLP-compliant study performed in accordance with standard methods, without deviations. The study is considered as reliable (Klimisch 1) and was selected as key study for the endpoint.
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