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EC number: 225-690-2 | CAS number: 5011-34-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 27 Sept. 2004 to 1st Oct. 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trimetazidine
- EC Number:
- 225-690-2
- EC Name:
- Trimetazidine
- Cas Number:
- 5011-34-7
- Molecular formula:
- C14H22N2O3
- IUPAC Name:
- 1-[(2,3,4-trimethoxyphenyl)methyl]piperazine
- Test material form:
- liquid
- Details on test material:
- No details are provided in the study report
Constituent 1
- Specific details on test material used for the study:
- No analytical data are provided on the test material in the study report. Batch N°538271/0124
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of the stock solution:
To prepare the stock solution, the test item will be dissolved or dispersed directly in test water. The obtained preparation will be agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item.
Water: filtered and dechlorinated potable water which is then softened by a Permo® water softening system. Half of the water passes directly into a reservoir tank attached to the water delivery system for culture while the remaining half passes through a deioniser producing ultra-pure water which is then fed into the reservoir tank. This results in a dilution of potable dechlorinated:deionised water giving a supply of the desirable hardness. The pH of the resulting solution is between 6.0 and 8.5 (generally in the range 7.5 - 8.0) and the total hardness is between 10 and 250 mg/L as CaC03 (generally in the range 130 - 170 mg/L as CaC03).
Preparation of the test solutions:
Test solutions were prepared by further dilution of the stock solution with water or directly from the test item. A solution containing a maximum of 100 mg/L of the test item plus two other range-finding concentrations was prepared. Test vessels (aquariums) were filled directly from the test solution containers immediately after preparation. The same vessels were used throughout the test.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Justification of this choice: the rainbow trout is a fish species generally accepted by regulatory authorities for this type of study and is recommended in OECD and EEC guidelines.
Origin: CIT, BP 563, 27005 Evreux, France; the eggs are obtained from Viviers de France, Pisciculture des Fontaines, 64490 Sarrance, France.
Allocation to study: animals were selected using a hand-net, without preference, and randomly assigned to test vessels.
Number of animals: four groups of seven fish.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Between 154 and 155mg/L of CaCO3 in the control flask
- Test temperature:
- Between 14.0 and 14.8°C
- pH:
- Between 7.66 and 8.72
- Dissolved oxygen:
- Between 7.4 and 10.0 mg/L
- Salinity:
- Not specified (freshwater)
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal concentrations : 0 ; 1 ; 10 & 100 mg/L
- Details on test conditions:
- Light/dark cycle: 16/8 hours.
Loading: up to 1 g of fish per liter of test solution. The animals were between 4 and 6 cm in length.
Food: During culture, animals were fed trout chow twice daily, ceasing at least 24 hourg before the start of the test. Animals were not fed during the test. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Physicochemical parameters
Time (hours)
Temperature (°C)
pH
Oxygene rate (mg/L)
Water hardness (mg/L of CaCO3)
Control
0*
14,0
7,45
9.9
154
24
14,5
7,87
8,0
48
14,6
7,66
7,9
72
14,3
7,73
7,8
96
14,3
7,93
7,6
155
Highest nominal concentration : 100mg/L until T96h
0*
14,2
8,72
10,0
24
14,6
8,04
7,8
48
14,8
7,77
8,0
72
14,4
7,77
7,6
96
14,3
7,90
7,4
* Parameter measured between T0 and T2 hours
Table 2:Summary of mortality
Observation time (hours)
Mortality at each nominal concentration (mg/L)
n fishes on 7 fishes
Control
1
10
100
0
0
0
0
0
24
0
0
0
0
48
0
0
0
0
72
0
0
0
0
96
0
0
0
0
Table 3: Observation of clinical signs
N fishes on 7 fishes
Nominal concentration (mg/L)
Time (hours)
NTR
Loss of eq.
fish on the surface
Fish at the bottom of the aquarium
Hyper.
vertical swim
swimming on the back
spasms
Loss
of color
quick breathing
Imm.
death
0
0 to 96h
0
0
0
0
0
0
0
0
0
0
0
0
1
0 to 96h
0
0
0
0
0
0
0
0
0
0
0
0
10
0 to 96h
0
0
0
0
0
0
0
0
0
0
0
0
100
0 to 96h
0
0
0
0
0
0
0
0
0
0
0
0
NTR: Nothing to Report
Loss of eq.: Loss of equilibrium
Hyper.: Hyperactive fish
Imm. :Immobilisation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest concentration without clinical effect and mortality at T96h was 100 mg/L.
The lowest concentration with 100% mortality at T96h was > 100 mg / L.
LC50s at 24h, 4811, 72h and 96h were > 100 mg / L. - Executive summary:
An acute toxicity test was performed on rainbow trout under static conditions according the OECD guidline 203. No toxicity on fish of the test item was observed during the test.
The highest concentration without clinical effect and mortality at T96h was 100 mg/L.
The lowest concentration with 100% mortality at T96h was > 100 mg / L.
LC50s at 24h, 4811, 72h and 96h were > 100 mg / L.
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