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EC number: 228-668-0 | CAS number: 6320-14-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to aquatic invertebrates:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below:
1.Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material. The nominal concent ration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be 100 mg/L. Also the substance was found to be not rapidly degradable.Thus according to CLP Criteria for aquatic classification of the substance, it is concluded that test material exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category.
EC50 (48 hours) Experimental = 100 mg/L
2.Determination of the inhibition of the mobility of daphnids was carried out with the substance,according to OECD Guideline 202.
The test substance was tested at the concentrations 0, 6, 10, 17, 29, 50 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance,1-(3-chloropropyl)-1,3-dihydro-2H-benzimidazol-2-one, in Daphnia magna was determined to be 29.8 mg/L for immobilisation effects.
This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic Chronic category 3 as per the CLP criteria.
Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 29 to 100 mg/
L. Thus, comparing this value with the criteria of CLP regulation,
1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified
for short term toxicity for aquatic invertebrate .Hence,based on the data available for functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.
Toxicity to aquatic algae and cyanobacteria:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below.
1.The algal growth inhibition test was performed using as test material on Chlorella sp .
Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .
The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.
Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.
2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.
The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.
This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.
Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified
for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.
.
Additional information
Short-term toxicity to aquatic invertebrates:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below:
1.Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material. The nominal concent ration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be 100 mg/L. Also the substance was found to be not rapidly degradable.Thus according to CLP Criteria for aquatic classification of the substance, it is concluded that test material exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category.
EC50 (48 hours) Experimental = 100 mg/L
2.Determination of the inhibition of the mobility of daphnids was carried out with the substance,according to OECD Guideline 202.
The test substance was tested at the concentrations 0, 6, 10, 17, 29, 50 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance,1-(3-chloropropyl)-1,3-dihydro-2H-benzimidazol-2-one, in Daphnia magna was determined to be 29.8 mg/L for immobilisation effects.
This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as Aquatic Chronic category 3 as per the CLP criteria.
Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 29 to 100 mg/
L. Thus, comparing this value with the criteria of CLP regulation,
1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified
for short term toxicity for aquatic invertebrate .Hence,based on the data available for functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.
Toxicity to aquatic algae and cyanobacteria:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride (6320 -14 -5).The studies are as mentioned below.
1.The algal growth inhibition test was performed using as test material on Chlorella sp .
Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .
The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.
Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.
2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.
The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.
This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.
Thus, based on the above summarised studies, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified
for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemical1,3,3-trimethyl-2-[(1E)-3-[(2E)-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium chloride can be classified as Aquatic Chronic category 3 as per the CLP criteria.
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