Ammonium iodide

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates

An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test chemical solution was prepared by dissolving 2 mg of the test chemical in 20 ml of ADaM’s media achieving test concentrations of 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical solution. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, pH 7.1, hardness of water 153.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. The daphnids were moving slowly as compared to control. No immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be 100 mg/L.

Toxicity to aquatic algae and cyanobacteria

An acute test was conducted for 72 hrs for assessing the effect of test chemical on green algae (Experimental study report, 2018). The test was performed in accordance to OECD Guideline 201 (Alga, Growth Inhibition Test). Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) of strain 86.81 SAG obtained from Institute of botany of the ASCR with an initial biomass conc. 5000 cells /ml was used as a test organism for the study. The solution 100 mg/l was prepared by dissolving test chemical in OECD growth medium. Nominal test chemical conc. used for the study was 100 mg/l, i.e, limit test was conducted. Study was performed using Desmodesmus subspicatus as a test organism in a static system. Desmodesmus subspicatus were exposed to test chemical in 50 ml glass vessel in a volume of 15 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 23±2°C with a continuous light intensity of 6000-8000 lx, respectively. Alongwith the test chemical, one positive control Potassium dichromate (K2Cr2O7) and control containing OECD medium without test chemical were also run simultaneously. Cell counting was carried out using electronic particle counter. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. On the basis of the effect of test chemical on the growth rate of the test organism Desmodesmus subspicatus, the 72hr Inhibition percentage (I%) value was determined to be 1.1% at 100.0 mg/l of test chemical concentration. Thus, the 72 hr median effect concentration (EC50) was evaluated to be > 100 mg/l., indicating that the test chemical is non-toxic to aquatic algae and therefore considered to be 'not classified' as per the CLP classification criteria.

Additional information

Short term toxicity to aquatic invertebrates

An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test chemical solution was prepared by dissolving 2 mg of the test chemical in 20 ml of ADaM’s media achieving test concentrations of 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical solution. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, pH 7.1, hardness of water 153.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. The daphnids were moving slowly as compared to control. No immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be 100 mg/L.

Toxicity to aquatic algae and cyanobacteria

An acute test was conducted for 72 hrs for assessing the effect of test chemical on green algae (Experimental study report, 2018). The test was performed in accordance to OECD Guideline 201 (Alga, Growth Inhibition Test). Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) of strain 86.81 SAG obtained from Institute of botany of the ASCR with an initial biomass conc. 5000 cells /ml was used as a test organism for the study. The solution 100 mg/l was prepared by dissolving test chemical in OECD growth medium. Nominal test chemical conc. used for the study was 100 mg/l, i.e, limit test was conducted. Study was performed using Desmodesmus subspicatus as a test organism in a static system. Desmodesmus subspicatus were exposed to test chemical in 50 ml glass vessel in a volume of 15 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 23±2°C with a continuous light intensity of 6000-8000 lx, respectively. Alongwith the test chemical, one positive control Potassium dichromate (K2Cr2O7) and control containing OECD medium without test chemical were also run simultaneously. Cell counting was carried out using electronic particle counter. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. On the basis of the effect of test chemical on the growth rate of the test organism Desmodesmus subspicatus, the 72hr Inhibition percentage (I%) value was determined to be 1.1% at 100.0 mg/l of test chemical concentration. Thus, the 72 hr median effect concentration (EC50) was evaluated to be > 100 mg/l., indicating that the test chemical is non-toxic to aquatic algae and therefore considered to be 'not classified' as per the CLP classification criteria.

On the basis of the available information of aquatic toxicity studies, it can be concluded that the test chemical was considered as non-toxic to aquatic organisms at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP criteria.