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EC number: 245-010-8 | CAS number: 22457-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15.10.1996 to 13.11.1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Inhibition control in Ready Biodegradability test.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Lot No.: 263992
Purity: 96.3% (GC, sum of 2 isomers) - Analytical monitoring:
- not required
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aire) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Nominal and measured concentrations:
- 100 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- Aniline
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Comparison of respiration of flasks containing test chemical + positive control and flasks containing positive control only
- Details on results:
- The curves obtained with Aniline alone and with STEMONE+Aniline show no significant toxic effect of STEMONE on the microorganisms at the test concentration (100mg/L) - see illustration section.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The toxicity of STEMONE to microorganisms was assessed in an inhibition control of a standardised ready test performed according to OECD 301F. The curves obtained with Aniline alone and with STEMONE+Aniline show no significant toxic effect of STEMONE on the microorganisms at the test concentration of 100mg/L. This concentration can be considered the NOEC for the toxicity to STP microorganisms.
- Executive summary:
The method used is basically the one described under No. 301 F in the OECD Guidelines for Testing of Chemicals. A measured volume of inoculated mineral medium, containing a known concentration of test substance (100 mg/l) as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature (± 1°C) for up to 28 days. The consumption of oxygen is determined by measuring the quantity of oxygen (produced electrolytically) required to maintain constant the gas volume in the respirometer flask. Evolved carbon dioxide is absorbed in soda lime pellets. The amount of oxygen taken up by the microbial population during biodegradation of the test chemical (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD (Theoretical Oxygen Demand, calculated from the elemental composition, assuming that carbon is oxidized to carbon dioxide and hydrogen to water) .
STEMONE undergoes almost no (1%) biodegradation after 28 days in the test conditions.
Thus, STEMONE should be regarded as not readily biodegradable according to this test.
The curves obtained with Aniline alone and with STEMONE+Aniline show no significant toxic effect of STEMONE on the microorganisms at the test concentration of 100 mg/L
Reference
RESULTS
Oxygen uptakes, as read on the SAPROMAT meters, are corrected:
- By deducting the basic oxygen uptake of sludge (flasks 2/5 and 2/6)
- proportionally to account for the differences between actual and measured concentrations of the test and reference substances.
In flasks containing aniline alone, pH remained roughly at the same level: this suggests that the final form of nitrogen is partly one of the acid forms NO2or NO3 –and partly the alkaline ion NH4 +.The Theoretical Oxygen Demand (ThOD) corresponding to NO3 -was used for the calculation of the biodegradation rates of aniline. Using the ThOD corresponding to NH4+would lead to higher degradation rates. In both case, degradation of Aniline exceeds 40% after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified and the test is considered as valid.
COMMENTS AND CONCLUSIONS
STEMONE undergoes almost no (1%) biodegradation after 28 days in the test conditions.
Thus, STEMONE should be regarded as not readily biodegradable according to this test.
The curves obtained with Aniline alone and with STEMONE+Aniline show no significant toxic effect of STEMONE on the microorganisms at the test concentration.
Description of key information
In a standardised ready biodegradability screening study (Rudio 1996), no toxicity was observed in an inhibition control at a test concentration of 100 mg/L. This concentration can be considered as a NOEC for the toxicity to STP microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
In the Biodegradation study, the curves obtained with Aniline alone and with STEMONE+Aniline show no significant toxic effect of STEMONE on the microorganisms at the test concentration of 100mg/L.
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