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Diss Factsheets
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EC number: 274-977-9 | CAS number: 70880-03-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Commission Regulation (EU) 2016/266
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- 0 h, 72 h
- Vehicle:
- no
- Details on test solutions:
- The stock solution of the test substance was prepared in the test medium. 100 mg of the test substance was weighted into 1000 ml of the test medium for the preliminary test and 25 mg of the test substance was weighted into 250 ml of the test medium for the limit test.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM - Common name: Desmodesmus subspicatus - Strain: Brinkmann 1953/SAG 86.81 - Source (laboratory, culture collection): The Botanic Institute of the Czech Academy of Science - Age of inoculum (at test initiation): 4 months - Method of cultivation: indirect daylight at laboratory temperature
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 21 - 23°C
- pH:
- 7.5 - 7.7
- Nominal and measured concentrations:
- The analytical determination of the test substance concentrations was performed at the beginning and at the end of the test. The samples for analysis were taken from the highest (100 mg/l), the lowest (1 mg/l) test concentration and from the highest concentration (100 mg/l) without algae.
- Details on test conditions:
- TEST SYSTEM - Test vessel: Erlenmeyer flask - Aeration: stopper venting air - Initial cells density: 5000 cells/ml - No. of vessels per concentration (replicates): 3 (preliminary test), 6 (limit test) - No. of vessels per control (replicates): 3 (preliminary test), 6 (limit test) GROWTH MEDIUM - Standard medium used: yes TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: deionized water - Conductivity: < 5 µS/cm - Culture medium different from test medium: no OTHER TEST CONDITIONS- Photoperiod: continuous light - Light intensity and quality: 4440 to 8880 lux TEST CONCENTRATIONS - Range finding study: 1, 5, 10, 50, 100 mg/l
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 > 100 mg/l (72 h, growth rate)EC50 > 100 mg/l (72 h, yield inhibition)NOEC < 100 mg/l (72 h, growth rate)NOEC < 100 mg/l (72 h, yield inhibition)
- Executive summary:
The preliminary test was performed in a range of test substance nominal concentrations 1 – 100 mg/l. The analytical results showed that the test substance, Reactive Violet 1, is sufficiently stable in the test medium at conditions of the test at current concentration range. The highest inhibition of growth rate was 15.2 % and the highest inhibition of yield was 43.1 % in the preliminary test. Based on no toxicity of the test substance lower than 50 % found in the preliminary test, the limit test was performed subsequently. The concentration of 100 mg/l was tested in the limit test. The inhibition of growth rate was 16.4 % and the inhibition of yield was 46.4 %.
Test results:
EC50 > 100 mg/l (72 h, growth rate)
EC50 > 100 mg/l (72 h, yield inhibition)
NOEC < 100 mg/l (72 h, growth rate)
NOEC < 100 mg/l (72 h, yield inhibition)
Reference
Tab No. 1: Mean cell density - preliminary test
nominal concentration | mean cell density in 1 ml | |||
0 h | 24 h | 48 h | 72 h | |
100 mg/l | 5000 | 12500 | 56250 | 104167 |
50 mg/l | 5000 | 12500 | 58333 | 114583 |
10 mg/l | 5000 | 14583 | 62500 | 110417 |
5 mg/l | 5000 | 12500 | 60417 | 114583 |
1 mg/l | 5000 | 14583 | 70833 | 125000 |
control | 5000 | 20833 | 79167 | 179167 |
Tab No. 2: Mean cell density - limit test
nominal concentration | mean cell density in 1 ml | |||
0 h | 24 h | 48 h | 72 h | |
100 mg/l | 5000 | 22917 | 46875 | 106250 |
control | 5000 | 23958 | 85417 | 193750 |
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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