1-chlorooctane

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorooctane

EC Number:

203-915-5

EC Name:

1-chlorooctane

Cas Number:

111-85-3

Molecular formula:

C8H17Cl

IUPAC Name:

1-chlorooctane

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Details on preparation and application of test substrate:

Test item was mixed with quartz sand and subsequently thoroughly mixed with the basic substrate.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Animals were bred at the testing facility. They were kept in a mixture of peat and cow dung. Animals that were used for the test, were at least 2 months old, had a clitellum and weighed between 300 and 600 mg.

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 +/- 2°C

pH:

at test start: 5.7

Moisture:

approx. 40% based on dry weight

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Makrolon cages covered with perforated plexiglass window
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 50% peat, 50% dried horse or cow dung

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400-600 Lux

Nominal and measured concentrations:

nominal: 100, 170, 300, 550 and 1000 mg/kg

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

100 mg/kg soil dw

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

216 mg/kg soil dw

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC100

Effect conc.:

300 mg/kg soil dw

Basis for effect:

mortality

Effect concentrations 4

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

220 mg/kg soil dw

Basis for effect:

mortality

Results with reference substance (positive control):

LC0 (14d): 30 mg/kg dw
LC100 (14d): 100 mg/kg dw

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the study the test item revealed an LC0 of 100 mg/kg soil dw, an LC50 of 216 mg/kg soil dw and an LC100 of 300 mg/kg soil dw after 14 days.