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EC number: 240-596-1 | CAS number: 16529-56-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- short-term repeated dose toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- No data
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Only one dose was tested. The purity is not indicated. Few data are available. No conclusion can be made.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 970
- Report date:
- 1970
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Six ChR-CD male rats were exposed to the test substance an a 16-liter bell jar for four hours. The animals were exposed for four hours each day for two weeks (total of ten exposures). Control rats were exposed to oxygen and nitrogen for the same amount of time. The test substance was administered at a uniform rate into a jeated (140-165 °C ) stainless steel T-tube by a syringue drive and vaporized under prepurified nitrogen. The test substance vapors were mixed with oxygen and carried into the bell jar; houseline air was used as diluent to give the desired atmospheric concentration.
For analysis, gas samples were taken periodically from the chamber exit and analyzed by a gaz chromatographic method.
Three control and three test rats were sacrified after the tenth exposure; the remaining three control and three test rats were sacrified following a 14-day recovery period. Gross and histopathologic examinations were performed. - GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- 2-methyl-3-butenenitrile
- EC Number:
- 240-596-1
- EC Name:
- 2-methyl-3-butenenitrile
- Cas Number:
- 16529-56-9
- Molecular formula:
- C5H7N
- IUPAC Name:
- 2-methylbut-3-enenitrile
- Details on test material:
- - Name of test material (as cited in study report): 2-methyl-3-butenenitrile (2M3BN)
- Substance type: No data
- Physical state: No data
- Analytical purity: No data
- Impurities (identity and concentrations): No data
- Composition of test material, percentage of components: No data
- Isomers composition: No data
- Purity test date: No data
- Expiration date of the lot/batch: No data
- Stability under test conditions: No data
- Storage condition of test material: No data
- Other: No data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: ChR-CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: 250-289 grams
- Fasting period before study: No data
- Housing: in cages
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: No data
ENVIRONMENTAL CONDITIONS
No data
IN-LIFE DATES: No data
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Remarks:
- houseline air
- Vehicle:
- other:
- Remarks on MMAD:
- MMAD / GSD: No data
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
No data
TEST ATMOSPHERE
- Brief description of analytical method used: No data
- Samples taken from breathing zone: yes
VEHICLE
- Composition of vehicle (if applicable): houseline air was used as diluent to give the desired atmospheric concentration
- Concentration of test material in vehicle (if applicable): houseline air was used as diluent to give the desired atmospheric concentration
- Justification of choice of vehicle: the test substance was mixed with oxygen and houseline air was used as diluent to give the desired atmospheric concentration
- Lot/batch no. (if required): No data
- Purity: No data - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- For analysis, gas samples were taken periodically from the chamber exit and analyzed by a gaz chromatographic method.
- Duration of treatment / exposure:
- Two weeks (total of ten exposures).
- Frequency of treatment:
- For four hours daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
560 ppm
Basis:
analytical conc.
- No. of animals per sex per dose:
- 6
- Control animals:
- yes
- Details on study design:
- No data
- Positive control:
- No data
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: No data
DETAILED CLINICAL OBSERVATIONS: Yes
- Time schedule: No data
BODY WEIGHT: Yes
FOOD CONSUMPTION:
- Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: No data
FOOD EFFICIENCY:
- Body weight gain in kg/food consumption in kg per unit time X 100 calculated as time-weighted averages from the consumption and body weight gain data: No data
WATER CONSUMPTION: No data
- Time schedule for examinations: No data
OPHTHALMOSCOPIC EXAMINATION: No data
HAEMATOLOGY: No data
CLINICAL CHEMISTRY: No data
URINALYSIS: No data
NEUROBEHAVIOURAL EXAMINATION: Yes
- Time schedule for examinations: No data
- Dose groups that were examined: No data
- Battery of functions tested: sensory activity / grip strength / motor activity / other: No data - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: Yes - Other examinations:
- No data
- Statistics:
- No data
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- no effects observed
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- not specified
- Details on results:
- CLINICAL SIGNS AND MORTALITY
Irregular respiration, hyper-sensitivity, red discharge around eyes, salivation, pale ears, pilo-erection during 5th, 6th and 8th exposures.
No mortality.
Normal gain weight gain post-exposure.
BODY WEIGHT AND WEIGHT GAIN
No weight gain during test period
GROSS PATHOLOGY
No evidence of primary injury by 2-methyl-3-butenenitrile.
HISTOPATHOLOGY: NON-NEOPLASTIC
No histological evidence of primary injury by 2-methyl-3-butenenitrile in any of the tissues (lungs, liver, spleen, kidney, testes and thymus) that were examined.
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
No additional data
Applicant's summary and conclusion
- Executive summary:
In a repeated dose toxicity, Six ChR-CD male rats were exposed to 2 -methyl-3 -butenenitrile for four hours each day for two weeks (total of ten exposures) at 560 ppm. Control rats were exposed to oxygen and nitrogen for the same amount of time. Three control and three test rats were sacrified after the tenth exposure; the remaining three control and three test rats were sacrified following a 14-day recovery period. Gross and histopathologic examinations were performed.
No clinical or pathologic indication of accumulation in exposed rats was observed. There were no histological evidence of primary injury by 2 -methyl-3 -butenenitrile, in any of the tissues examined. No mortality was observed.
Only one dose was tested, few data are available. Therefore non conclusion can be established.
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