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EC number: 206-750-7 | CAS number: 372-31-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August - September 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant guideline study
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethyl 4,4,4-trifluoroacetoacetate
- EC Number:
- 206-750-7
- EC Name:
- Ethyl 4,4,4-trifluoroacetoacetate
- Cas Number:
- 372-31-6
- Molecular formula:
- C6H7F3O3
- IUPAC Name:
- ethyl 4,4,4-trifluoro-3-oxobutanoate
- Test material form:
- other: clear liquid
- Details on test material:
- - Name of test material (as cited in study report): P5117
- Physical state: clear, colourless liquid
- Purity test date: 25-Jul-1995
- Expiration date of the lot/batch: no data
- Stability under test conditions: stable
- Storage condition of test material: stored at ambient temperature
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA1538, TA100, TA1535 and TA1537
- Details on mammalian cell type (if applicable):
- Type and identity of media:
Cultures of the histidine-dependent strains of Salmonella typhimurium were derived from cultures provided by Prof. Bruce Ames, University of California - Additional strain / cell type characteristics:
- DNA polymerase A deficient
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 liver fractions of rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- - Pretest: 5, 50, 500, 2500, 5000 ug/plate
- Main test: 50, 158, 500, 1580, 5000 ug/plate - Vehicle / solvent:
- Dimethylsulfoxide
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- A solution of P5117 was prepared in DMSO at 50 mg/ml, and four half-log dilutions were prepared from this solution. Aliquots (0.1 ml) of each concentration of P5117 were placed in sterile tubes. Molten, histidinedeficient top-agar (2 ml), maintained at 45°C, and bacterial suspension (0.1 ml) were then added. The tubes were mixed by inversion and 0.5 ml rat liver microsomal preparation (S9 mix) or 0.1M sodium phosphate buffer was added where appropriate. The tubes were again inverted to mix thoroughly and the contents poured onto plates containing solidified minimal medium (20 ml). Further plates were prepared without the inclusion of the test organisms to verify the sterility of the S9 mix and the test material. A control series of plates was prepared to confirm the inability of DMSO (0.1 ml) to induce reversion in the bacterial strains, and to provide a measure of the spontaneous mutation rates. Aliquots (0.1 ml) of a 10 Exp.-06 dilution of the overnight cultures were spread on the surface of plates of complete medium to measure the viability and celldensity of each culture.
All plates were prepared in triplicate, allowed to solidify and incubated at 37°C for 2 days. After incubation, numbers of revertant colonies were counted, either manually or with an automated colony counter. Total colonies on nutrient plates were counted in the same way. Growth of the background lawn of non-revertant cells on minimal plates was verified. Results obtained with all strains were confirmed in a second, independent experiment. All plates and tubes were identified by the use of numbers indelibly marked
on the plates and test tube racks.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA1538, TA100, TA1535 and TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
It was concluded that P5117 did not exhibit any mutagenic activity under the conditions of test. - Executive summary:
This reverse mutation assay was performed in 1995 as GLP-study in accordance with OECD- and EU-guidelines. Five Salm. typh. strains (TA1535, TA1537, TA1538, TA98 and TA100) were tested with and without metabolic activation. Test concentrations ranged from 50 ug/plate up to 5000 ug/plate. The test item was found to be not mutagenic in this bacterial reversion mutation assay.
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