Silicic acid, ethyl ester

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary source

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC, C.1

Principles of method if other than guideline:

Short term evaluation of test material on Brachydanio rerio was evaluated for 96 h

GLP compliance:

not specified

Analytical monitoring:

yes

Remarks:

Analytical assessment of the test substance concentrations was performed using carbon determination on a TOC-500 Infrared Analyzer

Details on test solutions:

The hard-to-dissolve test substance was added to the synthetic fresh water to provide a concentration of 1 g/l, and was stirred for 18 hours. Then the solution was
filtered and the carbon content was determined. A single test concentration of 119 mg/l was measured for this study

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
ACCLIMATION
- Acclimation period: 14 days
-Weight: 0.4 g

FEEDING DURING TEST
- Food type: Daily feed amounts of TetraMin®, approximately 1% of body weight, were supplied

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Test temperature:

20 deg C

Nominal and measured concentrations:

162, 126, 101 and 89 mg/l,

Details on test conditions:

Test vessel : 300-liter glass aquariums
Number of fish : Ten fish per 20 l test aquarium were exposed

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

119 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortalities were observed

Validity criteria fulfilled:

not specified

Conclusions:

LC0 observed after 96 h was 119 mg/l .No mortalities were observed.

Executive summary:

Short term toxicity of test material was evaluated on Brachydanio rerio for 96 hr under semistatic condition according to Directive 92/69/EEC, C.1 . The test substance was added to the synthetic fresh water to provide a concentration of 1 g/l, and was stirred for 18 hours. Then the solution was filtered and the carbon content was determined. A single test concentration of 119 mg/l was measured for this study. Fish were held in dechlorinated circulating potable water in 200 -liter stoneware tanks or in 300-liter glass aquariums, at 20 deg C. Daily feed amounts of TetraMin®, approximately 1% of body weight, were supplied. The specimens were pre-conditioned (treatment: 3x per week with malachite green) and subjected to a 14-day quarantine. They were used in testing only after this treatment. Only specimens displaying normal behavior at the beginning of the test and free of obvious disease were used in the study. Fish were not fed during the test. The testing density was 10 specimens per test tank; 0.4 g each. Ten fish per 20 l test aquarium were exposed for 96 hrs to a test substance concentration of 0 or 119 mg/l. LC0 observed after 96 h was 119 mg/l .No mortalities were observed.

Description of key information

Short-term toxicity to fish:

Short term toxicity of test material was evaluated on Brachydanio rerio for 96 hr under semistatic condition according to Directive 92/69/EEC, C.1 . The test substance was added to the synthetic fresh water to provide a concentration of 1 g/l, and was stirred for 18 hours. Then the solution was filtered and the carbon content was determined. A single test concentration of 119 mg/l was measured for this study. Fish were held in dechlorinated circulating potable water in 200 -liter stoneware tanks or in 300-liter glass aquariums, at 20 deg C. Daily feed amounts of TetraMin®, approximately 1% of body weight, were supplied. The specimens were pre-conditioned (treatment: 3x per week with malachite green) and subjected to a 14-day quarantine. They were used in testing only after this treatment. Only specimens displaying normal behavior at the beginning of the test and free of obvious disease were used in the study. Fish were not fed during the test. The testing density was 10 specimens per test tank; 0.4 g each. Ten fish per 20 l test aquarium were exposed for 96 hrs to a test substance concentration of 0 or 119 mg/l. LC0 observed after 96 h was 119 mg/l .No mortalities were observed.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

119 mg/L

Additional information

Short-term toxicity to fish:

The effect of test material on fish was observed based on the data for target chemical from secondary source as well as data for read across substances

For the target substance ,short term toxicity of test material was evaluated on Brachydanio rerio for 96 hr under semistatic condition according to Directive 92/69/EEC, C.1 . The test substance was added to the synthetic fresh water to provide a concentration of 1 g/l, and was stirred for 18 hours. Then the solution was filtered and the carbon content was determined. A single test concentration of 119 mg/l was measured for this study. Fish were held in dechlorinated circulating potable water in 200 -liter stoneware tanks or in 300-liter glass aquariums, at 20 deg C. Daily feed amounts of TetraMin®, approximately 1% of body weight, were supplied. The specimens were pre-conditioned (treatment: 3x per week with malachite green) and subjected to a 14-day quarantine. They were used in testing only after this treatment. Only specimens displaying normal behavior at the beginning of the test and free of obvious disease were used in the study. Fish were not fed during the test. The testing density was 10 specimens per test tank; 0.4 g each. Ten fish per 20 l test aquarium were exposed for 96 hrs to a test substance concentration of 0 or 119 mg/l. LC0 observed after 96 h was 119 mg/l .No mortalities were observed.

The above data was supported by data for structurally similar read across substance according to secondary source the Short term toxicity to fish Brachydanio rerio suggest the test was performed in semi-static condition for 96-hr exposure period and limit test following Directive 92/69EEC, C.1 (Huls AG, 1993a).During experiment the LC0 value for short term toxicity to fish for test material was determined to be 119 mg/l.

Based on the value, the test material was considered to be non-toxic to fish and can be considered to be not classified as per the CLP regulations.

The above data was further supported by data for functionally similar read across substance according to secondary source the Short term toxicity to fish Brachydanio rerio suggest the test was performed in semi-static condition for 96-hr exposure period and limit test following Directive 92/69/EEC, C.1 (Degussa-Huls AG, 1993c).During experiment the LC0 value for short term toxicity to fish for test material was determined to be 245 mg/l.

Based on the value, the test material was considered to be non-toxic to fish and can be considered to be not classified as per the CLP regulations.