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EC number: 405-370-0 | CAS number: 6334-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline, GLP Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 405-370-0
- EC Name:
- -
- Cas Number:
- 6334-25-4
- Molecular formula:
- C14H28N2O6
- IUPAC Name:
- N,N,N',N',-tetrakis(2-hydroxyethyl)hexanediamide
- Details on test material:
- - Name of test material (as cited in study report): Primid
- Substance type: organic
- Physical state: liquid
- Analytical purity: 99.8%
- Purity test date: not indicated
- Lot/batch No.: not indicated
- Expiration date of the lot/batch: not indicated
- Storage condition of test material: not indicated
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: 0, 4.6, 10, 22, 46, 100, 220 mg/L
Sampling at 0h and 72h
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 220 mg/L was prepared in test medium applying 5-6 minutes of magnetic stirring. Lower test concentrations were prepared by subsequent dilutions of the stock solution in test medium
The test medium source is M2 according to the ISO Standard.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Source: in house laboratory culture
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: according to OECD guideline
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): yes
- Any deformed or abnormal cells observed: not indicated
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 22.5-23C
- pH:
- test medium 8.3 +/- 0.2
during the test (0h and 72h measurements):
at 0 mg/L - 8.1 and 9.3
at 4.6 mg/L - 8.0 and 9.3
at 10 mg/L - 7.7 and 9.2
at 22 mg/L - 7.2 and 9.2
at 46 mg/L - 6.8 and 9.2
at 100 mg/L - 5.0 and 5.2
at 220 mg/L - 3.1 and 3.0 - Nominal and measured concentrations:
- nominal - 0, 4.6, 10, 22, 46, 100, 220 mg/L
measured - 0, 4.3, 10.1, 21.9, 44.8, 101, 224/233 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass 100mL with 50 mL medium
- Aeration: no
- Initial cells density: 10,000 cells/mL
- Control end cells density: 1,110,000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M2 according to the ISO Standard
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0h and 72h except temperature which was continuous
OTHER TEST CONDITIONS
- Sterile test conditions: not indicated
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 73-101 uE.m-2.s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : inhibition of cell growth and reduction of growth rate
- Measurement method: not indicated
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2X - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none indicated
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: appx 1 mg/L; with the EC50 a bit higher for growth rate than for biomass inhibition
- Other:
Any other information on results incl. tables
Nominal Conc - 0, 4.6, 10, 22, 46, 100, 220 mg/L
Grow Rate Inhib (% of controls) - 0, -0.3, 1.0, -0.4, -1.3, 84, 98
Biomass Inhib (% of controls) - 0, 0.5, 5.9, 1.3, -4.6, 97, 100
Growth Factor Control - 27.6
The 72h NOEC is 44.8 mg/L active ingredient (a.i.) based on measured concentration
Very possible that the effects observed at the highest test concentrations are related to the acidity of the test substance.
The measured pH values at 0 and 72 hours were:
mg/L 0h 72h
0 8.1 9.3
4.6 8.0 9.3
10 7.7 9.2
22 7.2 9.2
46 6.8 9.2
100 5.0 5.2
220 3.1 3.0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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