hexasodium (R)-carboxy(phosphono)methanolate (S)-carboxy(phosphono)methanolate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: conducted to OECD/EC guidelines with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): TK13061/2
- Physical state: Solid
- Lot/batch No.: LIC 613/04
- Storage condition of test material: room temperature in the dark

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Strain: CCAP 276/20
- Source (laboratory, culture collection): CCAP, Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

24 +/- 1 C

Nominal and measured concentrations:

Range finding test: nominal concentrations of 0.1, 1.0, 10 and 100 mg/L were exposed to Desmodesmus subspicatus for a period of 72 hours.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 ml
- Aeration: none
- Initial cells density: 4E3 (nominal)
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

OTHER TEST CONDITIONS
- Photoperiod: 72 hours
- Light intensity and quality: 7000 lux provided by warm white lighting (380 - 730 nm)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter Multisizer particle counter


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Remarks:

cell number and biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Remarks:

cell number and biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Remarks:

cell number and biomass

Details on results:

Range finding test concluded that a limit test had to be carried out.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- ErC50: 0.64 mg/L
- EyC50: 0.36 mg/L
- EbC50: 0.32 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test material on the growth of Desmodesmus subspicatus has been investigated and gave EC50 values of greater than 100 mg/L. Correspondingly the NOEC was 100 mg/L.

Executive summary:

In a 72 hour acute toxicity study, the cultures of Desmodesmus subspicatus were exposed to Trisodium [carboxy(oxido)methyl]phosphonate at nominal concentrations of 100 mg a.i./L under static conditions in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test).  The EC₅₀ value was >100 Mg a.i./L. 

 

Results Synopsis

 

Test Organism: Desmodesmus subspicatus

Test Type (Flowthrough, Static, Static Renewal): static

 

72 hr EC₅₀: > 100 mg a.i./L