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Reaction mass of sodium amino-bis{[4-(ethenylsubstituted)phenyl]diazenyl}-hydroxynaphthalenesulfonate and polysodium amino-{[4-(ethenylsubstituted)phenyl]diazenyl}-{[4-(ethenylsubstituted)-2-sulfonatophenyl]diazenyl}-hydroxynaphthalenesulfonate and polysodium amino-bis{[4-(ethenylsubstituted)-2-sulfonatophenyl]diazenyl}-hydroxynaphthalenesulfonate
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Guidelines followed
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- None
- Vehicle:
- yes
- Details on test solutions:
- None
- Test organisms (species):
- Lemna gibba
- Details on test organisms:
- None
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Frond number in each replicate was counted on days ‘0’, ‘3’, ‘5’ and ‘7’.
A representative sample (approximately 12 fronds) of pre-culture was checked for its average dry weight on day ‘0’ of exposure and dry weight of plant material from each test and control vessel on day ‘7’ of exposure.
Observation was performed on day ‘0’, ‘3’, ‘5’ and ‘7’ for the abnormal appearance or any developmental changes due to the effect of the test item (if any). - Hardness:
- None
- Test temperature:
- Temperature at the start (‘0’ Day) of the experiment in control & treatments from the bulk sample
Test Concentration (mg/L) Temperature (°C)
Control 23.1
0.3 23.2
0.96 22.5
3.07 23.1
9.83 22.5
31.46 22.4
100.66 22.5
Temperature at the end (‘7’Day) of the experiment in control & treatments
Test Concentration (mg/L) R1 R2 R3 R4 R5 R6 Range of Temperature (°C)
Control 25.7 25.6 25.4 25.4 25.3 25.3 25.3 - 25.7
0.3 25.4 25.3 25.2 - - - 25.2 - 25.4
0.96 25.3 25.3 25.4 - - - 25.3 - 25.4
3.07 25.4 25.4 25.4 - - - 25.4
9.83 25.6 25.7 25.6 - - - 25.6 - 25.7
31.46 25.7 25.6 25.6 - - - 25.6 - 25.7
100.66 25.6 25.7 25.7 - - - 25.6 - 25.7 - pH:
- pH at the start (‘0’ Day) of the experiment in control & treatments from the bulk sample
Test Concentration (mg/L) pH Values
Control 7.60
0.3 8.00
0.96 8.01
3.07 8.01
9.83 7.99
31.46 7.98
100.66 7.98
pH at the end (Day ‘7’) of the experiment in control & treatments
Test Concentration (mg/L) R1 R2 R3 R4 R5 R6 Range of pH
Control 8.79 8.80 8.77 8.80 8.71 8.80 8.71 - 8.80
0.3 9.38 9.36 9.36 - - - 9.36 - 9.38
0.96 9.24 9.26 9.26 - - - 9.24 - 9.26
3.07 9.00 9.04 9.04 - - - 9.00 - 9.04
9.83 9.03 9.05 9.05 - - - 9.03 - 9.05
31.46 8.83 8.81 8.72 - - - 8.81 - 8.83
100.66 8.70 8.71 8.72 - - - 8.70 - 8.72 - Dissolved oxygen:
- None
- Salinity:
- None
- Nominal and measured concentrations:
- Analytical verification of the test item in the exposure medium samples were carried out in the test item concentrations of low (0.3 mg/L), intermediate (9.83mg/L) and high (100.66 mg/L) on days ‘0’ and 7. The mean recoveries data are given below:
Day ‘0’ – 90.47 %, 91.36 % and 93.68 % for 0.3 mg/L, 9.83 mg/L and 100.66 mg/L test concentrations respectively.
Day ‘7’ – 89.36 %, 90.22 % and 92.02 % for 0.3 mg/L, 9.83 mg/L and 100.66 mg/L test concentrations respectively. - Details on test conditions:
- None
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 25.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 7.65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 19.43 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 5.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- None
- Results with reference substance (positive control):
- The sensitivity of the test system and reliability of the experimental technique was assessed with (3,5-Dichlorophenol) during May 2014 (Study No. 4521). This was performed in accordance with the following guideline: OECD Guidelines for the Testing of Chemicals, Section 2. Number 221, “Lemna sp. Growth Inhibition Test” Adopted on 23rd March 2006.
Lemna gibba (Duckweed) were exposed to the test item (3,5-Dichlorophenol) at different test concentrations viz., 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L. Initially on day ‘0’, 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium.
Prior to exposure Lemna gibba was pre-cultured in the 20X-AAP medium for period of ‘8’ days and the pre-culture was found healthy. After exposure, Lemna gibba was observed for frond count on days ‘3’, ‘5’ and ‘7’. Dry weight of the fronds were recorded on day ‘0’ (pre-culture representative samples) and day ‘7’.
Fronds were observed for their appearance on days ‘3’, ‘5’ and ‘7’. All fronds were found normal and healthy in all replicates of control and test concentrations except 6.30 mg/L where root length variation was observed in all the replicates on 3rd, 5th and 7th day.
The doubling time (Td) of frond number in the control group was calculated as 1.59 day-1 .
Frond Numbers:
From the average specific growth rate recorded with the different test concentrations viz., 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L, the test concentration that inhibits 50% growth (ErC50) was calculated as 4.38 mg/L.
The percent inhibition on the average specific growth rate on day ‘7’ for the test concentrations of 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L was calculated as 0.04%, 1.00%, 2.18%, 14.94% and 82.73 % respectively.
From the yield recorded with the different test concentrations viz., 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L the concentration that inhibited 50% yield (EyC50) was calculated as 3.01 mg/L.
The lowest observed effect concentration (LOEC) was calculated as 1.09 mg/L and the no observed effect concentration (NOEC) was calculated as 0.46 mg/L respectively.
Frond Dry Weight:
From the average specific growth rate recorded with the test concentrations viz., 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L, the concentration that inhibited 50% growth (ErC50) was calculated as 4.72 mg/L.
The percent inhibition on the average growth rate on day ‘7’ for the concentration of 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L was calculated as 0.39%, 1.11%, 7.65%, 10.39% and 74.18% respectively.
From the yield recorded with the test concentrations viz., 0.19, 0.46, 1.09, 2.63 and 6.30 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 2.92 mg/L.
The lowest observed effect concentration (LOEC) was calculated as 1.09 mg/L and no observed effect concentration (NOEC) was calculated as 0.46 mg/L respectively.
Based on the test results, ErC50 of 3,5-Dichlorophenol based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 4.38 mg/L with a lower confidence limit of 4.26 mg/L and the upper confidence limit of 4.51 mg/L.
EyC50 of 3,5-Dichlorophenol based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 3.01 mg/L with a lower confidence limit of 2.92 mg/L and the upper confidence limit of 3.11 mg/L.
ErC50 of 3,5-Dichlorophenol based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was calculated as 4.72 mg/L with a lower confidence limit of 4.56 mg/L and the upper confidence limit of 4.88 mg/L.
EyC50 of 3,5-Dichlorophenol based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was calculated as 2.92 mg/L with a lower confidence limit of 2.82 mg/L and the upper confidence limit of 3.03 mg/L. - Reported statistics and error estimates:
- Percent inhibition of Growth rate (Based on Frond Number)
ErC50 25.03 mg/L
Upper confidence limit 26.80 mg/L
Lower confidence limit 23.37 mg/L
Percent inhibition of yield (Based on Frond Number)
EyC50 7.65 mg/L
Upper confidence limit 8.13 mg/L
Lower confidence limit 7.19 mg/L
Percent inhibition of Growth rate (Based on Frond Dry Weight)
ErC50 19.43 mg/L
Upper confidence limit 21.04 mg/L
Lower confidence limit 17.94 mg/L
Percent inhibition of yield (Based on Frond Dry weight)
EyC50 5.02 mg/L
Upper confidence limit 5.33 mg/L
Lower confidence limit 4.72 mg/L
NOEC AND LOEC (Based on Frond Number)
NOEC 0.3 mg/L
LOEC 0.96 mg/L
NOEC AND LOEC (Based on Frond Dry weight)
NOEC 0.3 mg/L
LOEC 0.96 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- ErC50 of FAT 40865/A TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 25.03 mg/L with upper confidence limits of 26.80 mg/L and lower confidence limits of 23.37 mg/L.
- Executive summary:
Lemna gibba (Duckweed) were exposed to the test item (FAT 40865/A TE) at different test concentrations viz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L following OECD Guideline 221. Initially on day ‘0’, 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium. Prior to exposure, Lemna gibba was pre-cultured in the 20X-AAP medium for period of ‘9’ days and the pre-culture was found healthy. After exposure, Lemna gibba was observed for frond count on days ‘3’, ‘5’ and ‘7’. Dry weight of the fronds were recorded at start (pre-culture representative samples) and at the end of the test. Fronds were observed for their appearance on days ‘3’, ‘5’ and ‘7’. All fronds were found normal and healthy in all replicates of control and test concentrations except 31.46 and 100.66 mg/L where necrosis observed in all the replicates on 5th day and in addition to necrosis, root filaments were turned red in all the replicates on 7th day respectively. The doubling time (Td) of frond number in the control group was calculated as 1.61 day-1.
Frond Numbers:
From the average specific growth rate recorded with the different test concentrations viz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L, the test concentration that inhibited 50% growth (ErC50) was calculated as 25.03 mg/L with upper confidence limits of 26.80 mg/L and lower confidence limits of 23.37 mg/L.
The percent inhibition on the average specific growth rate on day ‘7’ for the test concentrations of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 0.23%, 4.57%, 6.42%, 25.84%, 59.09% and 80.99% respectively.
From the yield recorded with the different test concentrationsviz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L the concentration that inhibited 50% yield (EyC50) was calculated as 7.65 mg/L with upper confidence limits of 8.13 mg/L and lower confidence limits of 7.19 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentrations of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 0.73%, 13.50%, 18.43%, 56.75%, 87% and 96% respectively.
The no observed effect concentration (NOEC) was calculated as 0.3 mg/L and the low observed effect concentration (LOEC) was calculated as 0.96 mg/L respectively.
Frond Dry Weight:
From the average specific growth rate recorded with the test concentrations viz 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L, the concentration that inhibited 50% growth (ErC50) was calculated as 19.43 mg/L with upper confidence limits of 21.04 mg/L and lower confidence limits of 17.94 mg/L.
The percent inhibition on the average growth rate on day ‘7’ for the concentration of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 2.08, 6.25, 8.33, 50.00, 58.33 and 77.08% respectively.
From the yield recorded with the test concentrations viz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 5.02 mg/L with upper confidence limits of 5.33 mg/L and lower confidence limits of 4.72 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentrations of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 2.21, 16.57, 20.54, 84.35, 88.21 and 95.75% respectively.
The no observed effect concentration (NOEC) was calculated as 0.3 mg/L and the low observed effect concentration (LOEC) was calculated as 0.96 mg/L respectively.
Analytical verification of the test item in the exposure medium samples were carried out in the low (0.3 mg/L), intermediate (9.83 mg/L) and high (100.66 mg/L) concentration groups on days ‘0’ and ‘7’. The mean recoveries data are given below:
Day ‘0’ – 90.47, 91.36 and 93.68 % for 0.3, 9.83 and 100.66 mg/L test concentrations respectively.
Day ‘7’ – 89.36, 90.22 and 92.02 % for 0.3, 9.83 and 100.66 mg/L test concentrations respectively.
Reference
None
Description of key information
ErC50 of FAT 40865/A TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 25.03 mg/L with upper confidence limits of 26.80 mg/L and lower confidence limits of 23.37 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 25.03 mg/L
Additional information
Lemna gibba (Duckweed) were exposed to the test item (FAT 40865/A TE) at different test concentrations viz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L following OECD Guideline 221. Initially on day ‘0’, 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium. Prior to exposure, Lemna gibba was pre-cultured in the 20X-AAP medium for period of ‘9’ days and the pre-culture was found healthy. After exposure, Lemna gibba was observed for frond count on days ‘3’, ‘5’ and ‘7’. Dry weight of the fronds were recorded at start (pre-culture representative samples) and at the end of the test. Fronds were observed for their appearance on days ‘3’, ‘5’ and ‘7’. All fronds were found normal and healthy in all replicates of control and test concentrations except 31.46 and 100.66 mg/L where necrosis observed in all the replicates on 5th day and in addition to necrosis, root filaments were turned red in all the replicates on 7th day respectively. The doubling time (Td) of frond number in the control group was calculated as 1.61 day-1.
Frond Numbers:
From the average specific growth rate recorded with the different test concentrations viz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L, the test concentration that inhibited 50% growth (ErC50) was calculated as 25.03 mg/L with upper confidence limits of 26.80 mg/L and lower confidence limits of 23.37 mg/L.
The percent inhibition on the average specific growth rate on day ‘7’ for the test concentrations of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 0.23%, 4.57%, 6.42%, 25.84%, 59.09% and 80.99% respectively.
From the yield recorded with the different test concentrationsviz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L the concentration that inhibited 50% yield (EyC50) was calculated as 7.65 mg/L with upper confidence limits of 8.13 mg/L and lower confidence limits of 7.19 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentrations of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 0.73%, 13.50%, 18.43%, 56.75%, 87% and 96% respectively.
The no observed effect concentration (NOEC) was calculated as 0.3 mg/L and the low observed effect concentration (LOEC) was calculated as 0.96 mg/L respectively.
Frond Dry Weight:
From the average specific growth rate recorded with the test concentrations viz 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L, the concentration that inhibited 50% growth (ErC50) was calculated as 19.43 mg/L with upper confidence limits of 21.04 mg/L and lower confidence limits of 17.94 mg/L.
The percent inhibition on the average growth rate on day ‘7’ for the concentration of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 2.08, 6.25, 8.33, 50.00, 58.33 and 77.08% respectively.
From the yield recorded with the test concentrations viz.,0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 5.02 mg/L with upper confidence limits of 5.33 mg/L and lower confidence limits of 4.72 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentrations of 0.3, 0.96, 3.07, 9.83, 31.46 and 100.66 mg/L was calculated as 2.21, 16.57, 20.54, 84.35, 88.21 and 95.75% respectively.
The no observed effect concentration (NOEC) was calculated as 0.3 mg/L and the low observed effect concentration (LOEC) was calculated as 0.96 mg/L respectively.
Analytical verification of the test item in the exposure medium samples were carried out in the low (0.3 mg/L), intermediate (9.83 mg/L) and high (100.66 mg/L) concentration groups on days ‘0’ and ‘7’. The mean recoveries data are given below:
Day ‘0’ – 90.47, 91.36 and 93.68 % for 0.3, 9.83 and 100.66 mg/L test concentrations respectively.
Day ‘7’ – 89.36, 90.22 and 92.02 % for 0.3, 9.83 and 100.66 mg/L test concentrations respectively.
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