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EC number: 246-916-6 | CAS number: 25377-72-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 June 1983 to October 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Bridges, B. A. (1972) Simple bacterial systems for detecting mutagenic agents. Lab. Practice, 21 413-416.
Ames, B.N., McCann, J. and Yamasaki, E. (1975) Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-Microsome Mutagenicity Test. Mutation Res., 31, 347-364. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pentene
- EC Number:
- 246-916-6
- EC Name:
- Pentene
- Cas Number:
- 25377-72-4
- Molecular formula:
- C5H10
- IUPAC Name:
- pent-1-ene
- Details on test material:
- - Name of test material (as cited in study report): Pentene-1.(n-amylene)
- Physical state: Clear, colourless volatile liquid.
- Analytical purity: 94.4%
- Lot/batch No.: ST83/065
- Expiration date of the lot/batch:
- Stability under test conditions: The test substance was judged to have been stable for the duration of this study. (Formulations) The stabilities of formulations of the test substance in Tween 80/ethanol was stable for at least one working day (7.5 h).
- Storage condition of test material: In the dark at ambient temperature.
Constituent 1
Method
- Target gene:
- Histidine operon (his) for Salmonella.
Tryptophan operon (trp) for E.Coli.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 fraction
- Test concentrations with justification for top dose:
- Without S9 mix: 62.5, 125, 250, 500 and 1000 ug/ml
With S9 mix: 62.5, 125, 250, 500, 1000, 2000 and 4000 ug/ml
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- (concurrent vehicle control)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Tween 80/ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- for S. typhimurium with or without S9
Migrated to IUCLID6: 1.7 µg/ml
- Untreated negative controls:
- yes
- Remarks:
- (concurrent vehicle control)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Tween 80/ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- for S. typhimurium with or without S9
Migrated to IUCLID6: 10 µg/ml
- Untreated negative controls:
- yes
- Remarks:
- (concurrent vehicle control)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Tween 80/ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: neutral red, 10 µg/ml
- Remarks:
- for S. typhimurium with or without S9
- Untreated negative controls:
- yes
- Remarks:
- (concurrent vehicle control)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Tween 80/ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: potassium dichromate, 10 µg/ml
- Remarks:
- for E. coli with or without S9
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100, E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- > 1000 µg/ml
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100, E. coli WP2 uvr A pKM 101
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- > 4000 µg/ml
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The results of this study indicate that when examined over a wide range of concentrations Pentene-1 did not induce reverse mutation in bacteria (both with and without metabolic activation). - Executive summary:
The mutagenic activity of Pentene-1 was investigated in agar layer cultures of Escherichia coli and Salmonella typhimurium bacterial tester strains. Assay was performed both in the presence and absence of S9 microsomal fraction obtained from a liver homogenate from rats pretreated with Aroclor 1254.
The results indicate that Pentene-1 did not induce mutation in bacteria) under the conditions of the assays described.
The test was conducted following to the similar methodologies as OECD testguideline 471. There are no information available regarding the testing facility therefore a reliability of 2 is assigned.
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