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EC number: 943-265-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 2015 - June 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MEP, P.R. China, the Guidelines for the Testing of Chemicals, Effects on Biotic Systems (the Second Edition), No. 209 “Activated Sludge, Respiration Inhibition Test”. (2013.8
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- TEST MATERIAL
- batch No.of test material: 3706OB
- Expiration date of the lot/batch: March 2019
- Purity: >99%
- Appearance: white powder
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature at 15-25°C. - Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: aeration tank of Shenyang North Sewage Treatment Plant
- Pretreatment: On the day of activated sludge collection, remove coarse particles by settling for 15 minutes, and decanting the upper layer of finer solids. The sludge was washed as followed. The sludge was first centrifuged for 5 minutes at ca. 2500 rpm to produce a clear supernatant and pellet of sewage solids. The supernatant liquid was discarded and the sludge was re-suspended in chlorine-free tap water, with shaking, and the wash-water was removed by re-centrifuging and discarding again. Re-suspend the sludge pellet in chlorine-free tap water, and the concentration of the re-suspended sludge was determined by drying method (10 ml suspended sludge with 3 replicate was dried at 105°C for 1.5h). From the measured results, the sludge was diluted further in chlorine-free tap water to obtain the required sludge solids concentration of 3 g/L.
- Preparation of inoculum for exposure: The activated sludge was fed with the synthetic sewage feed (50 mL synthetic sewage feed/L activated sludge) and continuously aerated at the test temperature (19.8~21.3°C) overnight prior to use in the test.
- Initial biomass concentration: Before the activated sludge was used as the inoculums, the concentration of the sludge was determined again by drying method, and the concentration was adjusted to be 3.0 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- Not required
- Test temperature:
- 20±2°C in a temperature controlled room, actual recorded temperature was 19.8~21.6°C.
- Dissolved oxygen:
- Not required
- Salinity:
- Not required
- Conductivity:
- Not required
- Nominal and measured concentrations:
- According to the results of the range-finding test, the definitive test was conducted with 6 concentrations of 0.100 mg/L, 0.496 mg/L, 2.46 mg/L, 12.2 mg/L, 60.5 mg/L and 300 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- No. of vessels per concentration (replicates): 5 replicates
- No. of vessels per control (replicates): 6 replicates without ATU and with ATU
- Sludge concentration (weight of dry solids per volume):
- Weight of dry solids per volume of reaction mixture per unit of time:
- Nutrients provided for bacteria:
- Nitrification inhibitor used : N-allylthiourea, the final concentration was 11.6 mg ATU/L in the test solutions
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Particulate matter:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
- Details on termination of incubation:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using fewer concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3,5-DCP), the nominal concentrations were 0.10, 0.30, 0.91, 2.7, 8.3 and 25 mg/L, one replicate at each test concentration.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 346.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 506.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 154.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Details on results:
- Based on the results of test item test, the EC50 (3 hours) of test item for three different respirations are as follows:
- EC50 (3h) Total = 346.1 mg/L with a 95% confidence limits of (214.5 - 676.6mg/L)
- EC50 (3h) Heterotrophic = 50.6.3 mg/L with a 95% confidence limits of (311.0 -1061.6 mg/L)
- EC50 (3h) Nitrification = 154.6 mg/L with a 95% confidence limits of (60.7 - 826.9 mg/L) - Results with reference substance (positive control):
- The validity criteria for the reference item EC50 values were satisfied:
EC50 Total = 7.4 mg/L
EC50 Heterotrophic = 10.6 mg/L
EC50 Nitrification = 3.5 mg/L
The specific oxygen uptake rate of the blank controls (without the test item or reference item) was 25.3 mg/(g·h) oxygen. The coefficient of variation of oxygen uptake rate in control replicates was 8.2% at the end of definitive test. The validity criteria have therefore been satisfied. - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of test item test, the EC50 (3 hours) of test item are as follows:
- EC50 (mg/L) Total = 346.1 mg/L with a 95% confidence limits of (214.5 - 676.6mg/L)
- EC50 (mg/L) Heterotrophic = 50.6.3 mg/L with a 95% confidence limits of (311.0 -1061.6 mg/L)
- EC50 (mg/L) Nitrification = 154.6 mg/L with a 95% confidence limits of (60.7 - 826.9 mg/L) - Executive summary:
The study was conducted to determine the inhibition effects of XA 31 micro-organisms from activated sludge by measuring their respiration rate (including total, heterotrophic only and that due to nitrification) following an exposure time of 3 hours. The study was performed according to OECD Guideline 209 (2013) and was compliant with the GLP.
Based on the results of previous preliminary tests, the definitive test was conducted with 6 concentrations of 0.100 mg/L, 0.496 mg/L, 2.46 mg/L, 12.2 mg/L, 60.5 mg/L and 300 mg/L (5 replicates for each concentration). Two sets of test item mixtures at each concentration were prepared, one set of test mixture was used to determine inhibition effects of total oxygen uptake, another set contained 11.6 mg ATU/L was used to determine inhibition effects of heterotrophic respiration only, the differences between heterotrophic and the corresponding total respiration rates represent nitrification. Inoculums blank controls without ATU and containing ATU were performed with 6 replicates at the beginning, medium and end of the exposure period. The rate of respiration of each incubation mixture was determined after 3 hours contact time.
After 3 hours contact time at the highest tested concentration of 300 mg/L, the percentage inhibition were 51.3% for the total respiration, 43.6% for the inhibition of heterotrophic respiration and 64.7% for the inhibition of respiration due to nitrification.
Therefore based on the results of test item test, the EC50 (3 hours) of test item for three different respirations are as follows:
- EC50 (3h) Total = 346.1 mg/L with a 95% confidence limits of (214.5 - 676.6mg/L)
- EC50 (3h) Heterotrophic = 50.6.3 mg/L with a 95% confidence limits of (311.0 -1061.6 mg/L)
- EC50 (3h) Nitrification = 154.6 mg/L with a 95% confidence limits of (60.7 - 826.9 mg/L)
The validity criteria of the OECD guideline 209 were fulfilled, therefore this study is considered as reliable without restrictions.
Reference
Validity criteria:
- The specific oxygen uptake rate of the blank controls (without the test item or reference item) was 25.3 mg/(g·h) oxygen ( > 20 mg oxygen per one gram of activated sludge in an hour). The coefficient of variation of oxygen uptake rate in control replicates was 8.2% at the end of definitive test (> 30%.).
- The validity criteria for the reference item EC50 values were satisfied.
EC50 (mg/L) Total = 7.4 mg/L
EC50 (mg/L) Heterotrophic = 10.6 mg/L
EC50 (mg/L) Nitrification = 3.5 mg/L
Description of key information
One reliable study is available for the Reaction mass of AminoPhosphonium salt and BisphenolAF (Xa 31) for this endpoint.
The study was conducted to determine the inhibition effects of XA 31 micro-organisms from activated sludge by measuring their respiration rate (including total, heterotrophic only and that due to nitrification) following an exposure time of 3 hours. The study was performed according to OECD Guideline 209 (2013) and was compliant with the GLP.
Based on the results of previous preliminary tests, the definitive test was conducted with 6 concentrations of 0.100 mg/L, 0.496 mg/L, 2.46 mg/L, 12.2 mg/L, 60.5 mg/L and 300 mg/L (5 replicates for each concentration). Two sets of test item mixtures at each concentration were prepared, one set of test mixture was used to determine inhibition effects of total oxygen uptake, another set contained 11.6 mg ATU/L was used to determine inhibition effects of heterotrophic respiration only, the differences between heterotrophic and the corresponding total respiration rates represent nitrification. Inoculums blank controls without ATU and containing ATU were performed with 6 replicates at the beginning, medium and end of the exposure period. The rate of respiration of each incubation mixture was determined after 3 hours contact time.
After 3 hours contact time at the highest tested concentration of 300 mg/L, the percentage inhibition were 51.3% for the total respiration, 43.6% for the inhibition of heterotrophic respiration and 64.7% for the inhibition of respiration due to nitrification.
Therefore based on the results of test item test, the EC50 (3 hours) of test item for three different respirations are as follows:
- EC50 (3h) Total = 346.1 mg/L with a 95% confidence limits of (214.5 - 676.6mg/L)
- EC50 (3h) Heterotrophic = 50.6.3 mg/L with a 95% confidence limits of (311.0 -1061.6 mg/L)
- EC50 (3h) Nitrification = 154.6 mg/L with a 95% confidence limits of (60.7 - 826.9 mg/L)
However, as after 3 hours contact time at the highest tested concentration of 300 mg/L the percentage inhibition reached 51.3% for the total respiration, 43.6% for the inhibition of heterotrophic respiration and 64.7% for the inhibition of respiration due to nitrification, the final EC50 (3h) Total and Heterotrophic > 300 mg/L was preferred.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 300 mg/L
Additional information
the Key values are as follows ( not key data as < or > sign not allowed)
EC50 (3h) Total > 300 mg/L
EC50 (3h) Heterotrophic > 300 mg/L
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