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EC number: 281-650-4 | CAS number: 84000-94-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From February 16, 1994 to February 18, 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The test was conducted on a similar substance, the complete justification for the Read Across approach is reported at section 13. The original Reliability of the test is 1
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Ciba Geigy AG testing facility of the Product Ecology TF, Textile Dyes Division
- Method of breeding: Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 I of reconstituted water
- Feeding during test:
- Food type: feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantités that the food was consumed within 24h.
- Frequency: daily
ACCLIMATION
- Acclimation period: 24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 710 µm sieve.
- Acclimation conditions: 18 - 22 °C, The water was renewed partially three times weekly. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Theoretical water hardness: 136 mg/l CaC03
- Test temperature:
- 21 °C
- pH:
- 7.7 - 8.2
- Dissolved oxygen:
- 92-99%
- Nominal and measured concentrations:
- NOMINAL: 0.58, 1.0, 1.8, 3.2, 5.8 and 10.0 mg test article / I.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml bekers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 - 200 ml test medium
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 0.5 g test substance were mixed with 200 ml test medium and fill up to 500 ml with test medium.
The stock solution was homogenized for 5 minutes in the ultrasonic bath.
The test medium used had the following composition :
NaHC03: 65 mg/l
MgS04 X 7 H20 123.3 mg/l
KCl 5.8 mg/l
CaCI2 X 2 H20 294 mg/l
Na2SiO3 X 9H20 10 mg/l
NaN03 274 µg/l
KH2P04 143 µg/l
K2HP04 184 µg/l
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4 :1
- Na / K ratio: 10:1
- Conductivity:
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no ,7.8 - 8.2.
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: no data
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0mg test article / I nominal.
- Results used to determine the conditions for the definitive study: yes, based on a pretest. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limit: 1.3549 - 1.6868
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- ca. 3.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance shows a EC50(48h) = 1.5 mg/l
- Executive summary:
The acute toxicity of test substance to Daphnia magna was determined in a 48-hours static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0 mg test article / l, and a control without any additions. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 80.0 - 120.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations non alteration of the test substance after 48 hours was observed. In the control non immobilization of the Daphnia magna was observed after 48 hours. The EC0 value is at least 1.0 mg/l, but might even be higher than this concentration. The EC100 value is 3.3 mg/l after 48 hours.
Results :
(24h) EC0: > 1.0 mg/l, EC50: 2.5 mg/l, EC100: 5.8 mg/l
(48h) EC0: > 1.0 mg/l, EC50: 1.5 mg/l, EC100: = 3.3 mg/l, NOEC= 1.0 mg/l
Reference
Description of key information
EC50= 1.5 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.5 mg/L
Additional information
Experiment was conducted on Similar Substance 2 according to the guideline OECD 202
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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