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EC number: 200-665-9 | CAS number: 67-71-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14.12.2016 - 16.12.2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result using OECD guidelines.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- Determination of the inhibition of the mobility of Daphnids was carried out with the substance according to OECD Guideline 202.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The solution 100.0 mg/L was prepared by dissolving white powder in reconstituted water.
- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age: Young daphnids, aged less than 24 hours used at the start of the test and they should not be first brood progeny.
- Feeding during test : Without feeding
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- ± 1 h
- Test temperature:
- 20 °C ± 1 °C
- pH:
- Sample : pH = 7.7 did not change during the test,
Control: pH = 7.7 changed to pH = 7.6 during the test. - Dissolved oxygen:
- Higher than 7.9 mg/L both in the control and the sample.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml glass vessel
- Material, size, headspace, fill volume: 25 ml
- No. of organisms per vessel: 5 pieces
- No. of vessels per concentration (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.
Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).
Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.
- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.
OTHER TEST CONDITIONS
- Adjustment of pH: Without adjustment
- Photoperiod: No - Darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Inhibition % was calculated. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: IC5%
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Other details not known
- Details on results:
- After the exposure of test chemical for 48 hrs, only 5% inhibition were observed at 100 mg/l. Thus EC50 was consider to be > 100 mg/l.
- Results with reference substance (positive control):
- - Results with reference substance valid
- 24h EC50 = 0.79 mg/L K2Cr2O7 - Validity criteria fulfilled:
- yes
- Conclusions:
- After the exposure of test chemical with daphnia magna for 48 hrs, only 5% inhibition were observed at 100 mg/l. Thus EC50 was consider to be > 100 mg/l on the basis of mobility inhibition effects in a 48 hours of study.
- Executive summary:
Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202. A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated. After the exposure of test chemical with daphnia magna for 48 hrs, only 5% inhibition were observed at 100 mg/l. Thus EC50 was consider to be > 100 mg/l on the basis of mobility inhibition effects in a 48 hours of study. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna) and thus not classified as per the CLP classification criteria.
Reference
Description of key information
Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202. A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated. After the exposure of test chemical with daphnia magna for 48 hrs, only 5% inhibition were observed at 100 mg/l. Thus EC50 was consider to be > 100 mg/l on the basis of mobility inhibition effects in a 48 hours of study. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna) and thus not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Based on the effects observation on the mobility of aquatic invertebrate by the chemical exposure, studies were reviewed from various experimental sources for test chemical were studied and mention as below:
In the first key study from experimental report determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202. A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. The test was performed under static conditions in a fresh water system at a temperature of 20 °C± 1 °C. EC50 was calculated. After the exposure of test chemical with daphnia magna for 48 hrs, only 5% inhibition were observed at 100 mg/l. Thus EC50 was consider to be > 100 mg/l on the basis of mobility inhibition effects in a 48 hours of study. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna) and thus not classified as per the CLP classification criteria.
First experimental study was supported by the second study from secondary source. A 96 hr static toxicity test was conducted to determine the toxic effect of test chemical on mysid shrimp (Mysidopsis bahia). The test was conducted in 1-L uncovered glass beakers, each of which contained 900ml of test chemical and control seawater. There was no aeration. Dissolved oxygen (DO) concentrations were >97% of saturation at initiation of the test. Shrimp were not fed during the test. Based on the results of range finding study, shrimp were tested at nominal concentration of 1000ppm. The test concentration was prepared by adding 0.9184 grams of test chemical in directly to seawater. Exposure to test chemical did not appear to adversely affect mysid shrimp. After 72 hours of exposure, no mortality had occurred in 1,000 ppm and control. After 96 hours of exposure, mortality was 10% among mysids exposed to 1,000 ppm and among control mysids 5% mortality were observed. Therefore, the LC10 value for test chemical is considered to be 1000 mg/l on mysid shrimp (Mysidopsis bahia). Based on the mortality effect, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Thus based on the both studies from experimental report and secondary source, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.
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