Triphenylphosphine oxide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. (tester strains which were able to detect crosslinking/oxidising agents were not used)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

tester strains which were able to detect crosslinking/oxidising agents were not used

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine oxide

EC Number:

212-338-8

EC Name:

Triphenylphosphine oxide

Cas Number:

791-28-6

Molecular formula:

C18H15OP

IUPAC Name:

triphenylphosphine oxide

Details on test material:

- Name of test material (as cited in study report): Tri-phenyl-phosphinoxid
- Physical state: solid
- Purity: not reported

Method

Target gene:

his-

Species / strain

Species / strain / cell type:

other: S. typhimurium TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix from Aroclor 1254 induced rat livers

Test concentrations with justification for top dose:

4 - 2500 ug/plate

Vehicle / solvent:

acetone

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: without S-9 mix: N-Methyl-N'-nitro-N-nitroso-guanidine for all strains; with S-9 mix: cyclophosphamide for TA 100; 2-aminoanthracene for TA 100, TA 98, TA 1537

Details on test system and experimental conditions:

METHOD OF APPLICATION: plate incorporation, two independent trials

NUMBER OF REPLICATIONS: 3

Evaluation criteria:

substances were considered as positive in the Ames test if following criteria are fulfilled:
- doubling the spontaneous mutation rate
- dose-effect-relationship observed
- reproducibility of the results

Results and discussion

Test results

Species / strain:

other: S. typhimurium TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

2000 µg/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

The TS was neither mutagenic in the Ames-test with metabolic activation by S-9 mix, nor without.
The amount of revertants was in the range of the negative controls (differences not significant):

Negative control groups (means ± SD):
              with S-9            without S-9
TA98    test1: 33.25 ± 9.36      28.5  ± 5.8
             test2: 27    ± 4.08      34.75 ± 14.27   
TA100  test1: 141.25 ± 15.97     107   ± 7.96
             test2: 97.75 ± 14.73     153.25 ± 9.03
TA1537 test1: 8.25  ± 2.75      6.5   ± 3.42
             test2: 5.75  ± 1.71      4.5   ± 1.73

The TS demonstrated to be toxic for the Salmonella strains employed upon concentrations > 2000 µg/plate.

Positive controls showed a distinct increase in revertants in all experimental approaches.