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EC number: 222-813-1 | CAS number: 3618-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Not conducted under GLP, no information on test substance purity
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2'-[[5-acetamide-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-ethoxyphenyl]imino]diethyl diacetate
- EC Number:
- 235-475-5
- EC Name:
- 2,2'-[[5-acetamide-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-ethoxyphenyl]imino]diethyl diacetate
- Cas Number:
- 12239-34-8
- Molecular formula:
- C24H27BrN6O10
- IUPAC Name:
- 2,2'-[[5-acetamide-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-ethoxyphenyl]imino]diethyl diacetate
- Test material form:
- solid
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0, 10, 50, 100, 500, 1000 and 5000 µg/plate
- Vehicle / solvent:
- The substance was dissolved 50 mg/ml in dimethylsulfoxide and further diluited in the same solvent according the need.
Controls
- Negative solvent / vehicle controls:
- yes
- Details on test system and experimental conditions:
- Media: Vogel-Bonner E agar medium (VB) has the following compositlon:
MgSO4 x 7H20 0.2 g/l
citric acid x H2O 2 g/l
K2HPO4 10 g/l
NaNH4HPO4 x 4 H2O 3.5 g/l
glucose 2 g/l
Agar 15 g/l
It was prepared by mixing 750 ml of 2% molten autoclave sterillzed water agar wlth 250 ml of.4 times concentrated salt solution and 10 ml of 20 % glucose solution autoclaved separately.
For the antibacterlal test, histidine was also added to give a final concentratlon of 10 µg/ml.
10 cm plastic petri dishes were prepared containing 20 ml each of VB agar and used within 3 days from preparation.
Top agar was prepared by mlxing 100 ml of autoclave sterilised 0.6 % agar and 0.5 % NaCl in water with 10 ml of a millipore filter sterillzed 0.5 mM histidine, 0.5 mM blotln solutlon. Top agar was dlspensed in 2 ml amounts into 16x100 mm glass tubes kept at 42 °C.
TA1535, TA1537, TA1538 Ames strains were kept at 4 °C in deep soft nutrient agar, strains TA100 and TA98 were kept at -80 °C in nutrient broth plus ampicillin (20 µg/ml) and dimethysulfoxide (80 µg/ml).
The day before the test, fresh cultures were prepared by inoculating 10 ml of nurtrient broth with each strain, which were incubated overnight at 37 °C. Concurrently with each series of test, cultures were checked for relevant characters, namely:
-histidine requirement
-biotin requirement
- uvrB character
-rfa charcter
- presence or absence of the pKM101 prototrophy
-induced reversion rate by known mutagens
For testing the antibacterial activity :
0.1 ml of solvent containing the required amount of substance + 0.1 ml of a 10^-5 dilution of an overnight culture of TA1535 or TA98 containing between 100 and 500 colony forming units (CFU) + if requested S9 mix
were added in sequence to each top agar tube. Controls received 0.1 ml of the solvent mixture.
The contents of each tube were quickly mixed and poured on the surface of a histidine containing VB plate.
Colonies were counted after 24 hours of incubation at 37 °C. Tests were carried put inm triplicate.
For testing mutagenicity:
0.1 ml of solvent containing the required amounts of substance or 0.1 ml of sovlent alone + 0.1 ml of overnight culture of each strain containing 10^7 bacteria and if reuired 0.5 ml of S9 mix were added in sequence to each top agar tube. The contents of each tube were quickly mixed and pured on the surface of a VB plate.
Revertant colonies were counted after 2 days of incubation at 37 °C. Tests were carried out in triplicate.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- up to 5000 ug/plate
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Key result
- Species / strain:
- S. typhimurium, other: TA1535, TA1537
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
Applicant's summary and conclusion
- Conclusions:
- Under the study conditions, the substance was mutagenic in strains TA98 and TA100 and ambiguous in strain TA1538 with metabolic activation.
- Executive summary:
A study was conducted to determine the mutagenic potential of the test substance (purity not given), according to OECD Guideline 471. Five strains of Salmonella typhimurium (TA98, TA100, TA1535, TA1537 and TA 1538) were exposed to the test substance, dissolved in dimethylsulfoxide, at concentrations of 0, 10, 50, 100, 500, 1000 and 5000 µg/plate with or without metabolic activation (S-9 mix) for 48 h. Negative and positive control experiments were valid. Under the experimental conditions, the substance was mutagenic in strains TA98 and TA100, and ambiguous in TA1538 with metabolic activation. The mutagenicity is of frame shift type (Archroma, 1980).
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