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EC number: 443-090-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 14, 2002 to January 17, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: 88/302/EEC, L 133 Part C
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- The test substance was added to about 130 mL deionized water and stirred overnight before testing (equilibration phase).
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Type: mixed population of aquatic microorganisms (activated sludge).
- Origin: aeration tank of a waste water treatment plant treating predominantly domestic sewage (Wupper area water authority).
- Pretreatment: aeration of the activated sludge; daily feed with synthetic medium.
- pH of the suspension before application: 7.3 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- with permanent aeration
- Test temperature:
- 19.1-19.3°C
- pH:
- Test: 8.2-8.4
- Dissolved oxygen:
- Control:
Start = 6.2-6.3; End = 2.9-3.1
Reference substance:
Start = 6.4-7.8; End = 2.7-6.4
Test:
Start = 5.3-6.2; End = 2.7-3.6 - Nominal and measured concentrations:
- - Type of test material application: direct weighing
- Test concentrations (nominal): 100, 1000 and 10,000 mg/L
- Test substance concentration in physico-chemical oxygen consumption control (nominal): 10,000 mg/L
- Concentration of reference substance '3, 5 dichlorophenol' (nominal): 5, 10 and 20 mg/L - Details on test conditions:
- - Test concentration of the activated sludge: 520 mg/L suspended solids (this concentration was selected on the basis of strong respiration of the activated sludge).
- Reference substance (positive control):
- yes
- Remarks:
- 3, 5 dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Refer to table 1 under 'Any other information on results incl. tables'.
- Results with reference substance (positive control):
- Refer to table 1 under 'Any other information on results incl. tables'.
- Reported statistics and error estimates:
- The EC values were determined by statistical methods (probit analysis).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the EC50 of the test substance was > 10,000mg/L, indicating a low toxicity to microorganisms.
- Executive summary:
A study was performed to assess the toxicity of the test substance to sludge microorganisms. The study was conducted in accordance with Guideline 88/302/EEC, L 133 Part C, in compliance with GLP.
Activated sludge from domestic sewage was exposed to nominal concentrations of 0,100, 1000 and 10,000 mg/L for a period of 3 h. The respiration rates of each of these mixture were determined after an aeration period of 3 h and compared to data from the control and the reference substance 3,5 -dichlorophenol.
The reference substance 3,5 -dichlorophenol produced 18.7, 29.7 and 73.1% respiration inhibition at 5, 10 and 20 mg/L, respectively. The test substance produced 7.7, 0.0 and 7.7% respiration inhibition at 100, 1000 and 10,000 mg/L, respectively.
Under the test conditions, the EC50 of the test substance was > 10,000 mg/L, indicating a low toxicity to microorganisms.
Reference
Table 1: Inhibition of respiration:
Treatment group |
Concentration [mg/L] |
Respiratory rate mg/[L×h] |
% inhibition of respiration |
Control |
- |
39 |
- |
Test substance |
100 |
36 |
7.7 |
Test substance |
1,000 |
39 |
0.0 |
Test substance |
10,000 |
36 |
7.7 |
Reference substance (3, 5 dichlorophenol) |
5 |
31.7 |
18.7 |
Reference substance (3, 5 dichlorophenol) |
10 |
27.4 |
29.7 |
Reference substance (3, 5 dichlorophenol) |
20 |
10.5 |
73.1 |
Description of key information
Under the test conditions, the EC50 of the test substance was > 10,000 mg/L, indicating a low toxicity to microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
- EC10 or NOEC for microorganisms:
- 10 000 mg/L
Additional information
A study was performed to assess the toxicity of the test substance to sludge microorganisms. The study was conducted in accordance with Guideline 88/302/EEC, L 133 Part C, in compliance with GLP. Activated sludge from domestic sewage was exposed to nominal concentrations of 0,100, 1000 and 10,000 mg/L for a period of 3 h. The respiration rates of each of these mixture were determined after an aeration period of 3 h and compared to data from the control and the reference substance 3,5 -dichlorophenol. The reference substance 3,5 -dichlorophenol produced 18.7, 29.7 and 73.1% respiration inhibition at 5, 10 and 20 mg/L, respectively. The test substance produced 7.7, 0.0 and 7.7% respiration inhibition at 100, 1000 and 10,000 mg/L, respectively. Under the test conditions, the EC50 of the test substance was > 10,000 mg/L, indicating a low toxicity to microorganisms.
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