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Diss Factsheets
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EC number: 915-623-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Test material form:
- liquid: viscous
- Details on test material:
- - Substance type: Technical product.
- Storage condition of test material: No data.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan/CPB, Zeist, The Netherlands.
- Weight at study initiation: 209-214 g males / 159-168 g females.
- Fasting period before study: 18-19 hours prior to dosing until 5.5-6 hours after dosing.
- Housing: stainless steel wire cages with two or three animals per cage.
- Diet: ad libitum.
- Water: ad libitum.
- Acclimation period: five days.
ENVIRONMENTAL CONDITIONS
- Temperature: 21-23°C.
- Humidity: 40-70%.
- Air changes: approximately 16 air changes per hour.
- Photoperiod: 12 hours light / 12 hours dark.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 0.05, 0.1 and 0.2 g/ml corn oil.
- Amount of vehicle (if gavage): 5 ml/kg. - Doses:
- 250, 500, 1000 and 2000 mg/kg
- No. of animals per sex per dose:
- five males and five females per dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days.
- Frequency of observations and weighing: the rats were observed 0-0.5, 1.5, 3, 5-5.5, 24 and 48 hours after application and thereafter once daily till the end of the study. Rats were weighed one day before and at 2, 7 and 14 days after dosing.
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- >= 1 000 - <= 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Oral administration of a single dose of 2000 mg/kg killed all male and female rats within 5-48 hours after dosing. All animals dosed at lower levels survived the 14-day observation period.
- Clinical signs:
- other: Clinical signs at dose level 2000 mg/kg b.w.: clinical signs were slight to severe in intensity (e.g. apathy, abnormal gait and posture, decreased body tone, decreased respiratory rate, respiratory difficulties, decreased locomotor activity, positional pa
- Gross pathology:
- Post-mortem examination of male and female rates that died during the observation period revealed rigor, dark urine, small and maculate thymus
glands, enlarged and dark adrenals and food-filled stomachs. In the animals that survived the 14-day observation period, slightly darkened adrenals were observed in one male dosed at 1000 mg/kg. Adrenals were found to be enlarged in 2 females given 1000 mg/kg and in 1 female dosed at 250 mg/kg.
Applicant's summary and conclusion
- Interpretation of results:
- other: Based on Regulation 1272/2008/EC, the substance is classified as Acute Tox. 4, H302.
- Conclusions:
- In a GLP-compliant guideline study, acute oral LD50 of 2-amylanthraquinone in rats was calculated to be between 1000-2000 mg/kg bw. Based on this classification of the substance as Acute Tox. 4, H302 is warranted under Regulation 1272/2008/EC.
- Executive summary:
In a GLP-compliant OECD Guideline 401 study 2 -amylanthraquinone was administered by gavage in doses of 250, 500, 1000 and 2000 mg/kg bw to the groups of five males and five females. Administration of a single dose of 2000 mg/kg killed all male and female rats within 5-48 hours after dosing. All animals dosed at lower levels survived the 14-day observation period. Male rats dosed at 1000 mg/kg and females dosed at 500 or 1000 mg/kg lost some weight in the first few days after treatment.
The LD50 was calculated as 1000-2000 mg/kg bw. Based on this the substance should be classified as Acute Tox. 4, H302 (Harmful if swallowed) under Regulation 1272/2008/EC.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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