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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented study according to international accepted guidelines and GLP compliant.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report date:
1984

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2',4'-trichloroacetophenone
EC Number:
224-218-2
EC Name:
2,2',4'-trichloroacetophenone
Cas Number:
4252-78-2
Molecular formula:
C8H5Cl3O
IUPAC Name:
2-chloro-1-(2,4-dichlorophenyl)ethan-1-one
Test material form:
solid: crystalline
Details on test material:
Test item: 2,4,ω-Trichloroacetophenone (CAS No. 4252-78-2)
Molecular formula: C8H5Cl3O
Molecular weight: 223,5 g/mol
Colour: white
Purity: ~ 95 %
Storage temperature: 20 °C, keep it in dark place

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
Weight: 3,4 - 3,5 kg
Room temperature: 20 ± 2 °C
Lighting: 12 hours
Humidity: 55 ± 10 %

Test system

Vehicle:
other: Polyethylenglycol 400
Controls:
not required
Amount / concentration applied:
100 mg
Duration of treatment / exposure:
24 h
Observation period (in vivo):
1, 24, 48 and 72 h
Number of animals or in vitro replicates:
3
Details on study design:
24 hours before the treatment was dropped one drop 0,01 % fluorescens-sodium solution to the eyes. Only the unhurt animals were used for the test.Each left eyes were put 100 mg substance in 0,05 ml PEG 400. The control was the right eye. After 24 hours was washed each treated eyes with warm saline.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Remarks:
(opacity)
Basis:
mean
Score:
2.6
Max. score:
4
Reversibility:
not fully reversible within: 21 day
Irritation parameter:
iris score
Basis:
mean
Score:
1
Max. score:
1
Reversibility:
fully reversible within: 21 day
Irritation parameter:
other: redness
Basis:
mean
Score:
3
Max. score:
3
Reversibility:
not fully reversible within: 21 day
Irritation parameter:
chemosis score
Basis:
mean
Score:
3.6
Max. score:
4
Reversibility:
not fully reversible within: 21 day

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Remarks:
Migrated information
Conclusions:
In this in vivo eye irritation / corrosion test with test item: 2,4,ω-Trichloroacetophenone (CAS 4252-78-2) the results indicates that the test item is highly irritant. The test item should be classified into GHS Category 1, because the effects have not fully reserved within an observation period of 21 days.