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EC number: 274-490-1 | CAS number: 70236-60-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February from 3rd to 24th, 2005
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to internationally accepted testing guidelines and performed according to GLP. Nevertheless, only the summary of the test report is available, thus many details are missing.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Version / remarks:
- adopted 17 December 2001
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- Disodium [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)
- EC Number:
- 274-490-1
- EC Name:
- Disodium [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)
- Cas Number:
- 70236-60-1
- Molecular formula:
- C36H21CrN8O11S.2Na
- IUPAC Name:
- disodium [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)-
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Fasted females.
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- water
- Details on oral exposure:
- Test material was administered orally as a suspension in distilled water.
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3 female rats per dose
- Details on study design:
- Clinical signs and body weight development were monitored during the study.
All animals were subjected to gross necropsy.
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 500 mg/kg bw
- Based on:
- test mat.
- Mortality:
- There were no deaths.
- Clinical signs:
- other: A dark brown-coloured liquid discharge from the anus was noted in five animals during the day of dosing. One animal appeared normal throughout the study and the remaining animals appeared normal one day after dosing.
Any other information on results incl. tables
Individual Clinical Observations and Mortality Data
Dose level mg/kg bw | Animal number and sex | Effects Noted After Dosing (h) | Effects Noted During Period After dosing (Days) | ||||||||||||||||
1/2 | 1 | 2 | 4** | 1** | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||
2000 | 1-0 female | 0 | 0 | D* | D* | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2000 | 1-1 female | 0 | 0 | D* | D* | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2000 | 1-2 female | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2000 | 2-0 female | 0 | 0 | D* | D* | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2000 | 2-1 female | 0 | 0 | D* | D* | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2000 | 2-2 female | 0 | 0 | 0 | D* | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0 = No signs of systemic toxicity
D* = Dark brown-coloured liquid discharge from the anus
** = Bedding stained dark brown
Individual Bodyweights and Weekly Bodyweight Changes
Dose level mg/kg bw | Animal number and sex | Bodyweight (g) at Day | Bodyweight Gain (g) During Week | |||
0 | 7 | 14 | 1 | 2 | ||
2000 | 1-0 female | 196 | 228 | 236 | 32 | 8 |
2000 | 1-1 female | 206 | 242 | 267 | 36 | 25 |
2000 | 1-2 female | 218 | 256 | 273 | 38 | 17 |
2000 | 2-0 female | 203 | 229 | 240 | 26 | 11 |
2000 | 2-1 female | 205 | 246 | 262 | 41 | 16 |
2000 | 2-2 female | 211 | 231 | 240 | 20 | 9 |
Individual Necropsy Findings
Dose level mg/kg bw | Animal number and sex | Time of Death | Macroscopic Observations |
2000 | 1-0 female | Killed Day 14 | No abnormalities detected |
2000 | 1-1 female | Killed Day 14 | No abnormalities detected |
2000 | 1-2 female | Killed Day 14 | No abnormalities detected |
2000 | 2-0 female | Killed Day 14 | No abnormalities detected |
2000 | 2-1 female | Killed Day 14 | No abnormalities detected |
2000 | 2-2 female | Killed Day 14 | No abnormalities detected |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information according to the CLP Regulation (EC 1272/2008) Criteria used for interpretation of results: EU
- Conclusions:
- LD50 greater than 2500 mg/kg bw.
- Executive summary:
Method
The study was performed to assess the acute oral toxicity of the test material following a single oral administration in the Sprague-Dawley CD strain rat. The method followed the OECD Guidelines for the Testing of Chemicals No. 423 "Acute Oral Toxicity - Acute Toxic Class Method". A group of three fasted females was treated with the test material at a dose level of 2000 mg/kg bodyweight. The test material was administered orally as a suspension in distilled water. Clinical signs and bodyweight development were monitored during the study. All animals were subjected to gross necropsy.
Results
There were no deaths. A dark brown-coloured liquid discharge from the anus was noted in five animals during the day of dosing. One animal appeared normal throughout the study and the remaining animals appeared normal one day after dosing.
Individual body weights and weekly bodyweight changes were normal and individual necropsy revealed no abnormalities.
Conclusion
The acute oral median lethal dose (LD50) of the test material, in the female Sprague-Dawley CD strain rat, was estimated as being greater than 2500 mg/kg bodyweight.
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