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EC number: 221-221-0 | CAS number: 3033-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted in accordance with an appropriate OECD test guideline and to GLP. The test period was extended beyond the recommended 28 days as no degradation plateau had been reached on Day 28 of the study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- The test temperature fell below the minimum 20°C on some occasions.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,3-epoxypropyltrimethylammonium chloride
- EC Number:
- 221-221-0
- EC Name:
- 2,3-epoxypropyltrimethylammonium chloride
- Cas Number:
- 3033-77-0
- Molecular formula:
- C6H14NO.Cl
- IUPAC Name:
- N,N,N-trimethyl(oxiran-2-yl)methanaminium chloride
- Details on test material:
- - Name of test material (as cited in study report): 2,3-epoxypropyltrimethylammonium chloride
The study report indicates that purity of the test substance is 73.6%. However, this relates to the material as the marketed product in aqueous solution. Since the test concentrations were determined on the basis of carbon content, it is therefore considered appropriate to report the test substance as being equivalent to the registered substance. No information on impurities is available.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant at 65232 Taunusstein-Bleidenstadt
- Storage conditions: Aerated by humidified compressed air
- Storage length: No information
- Pretreatment: The activated sludge was washed three times with drinking water to mainting dissolved organic carbon levels below 5 mg DOC/l. The sludge was allowed to settle for 15 minutes and the supernatant discarded , then the concentrated sludge was made up to the original volume with drinking water. - Duration of test (contact time):
- 42 d
Initial test substance concentration
- Initial conc.:
- 685 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Solution 1: 8.5 g KH2PO4, 21.75 g K2HPO4, 33.5 g Na2HPO4.2H20, 0.5 g NH4Cl in 1 litre deionised water
Solution 2: 36.43 g CaCl2.2H20 in 1 litre deionised water
Solution 3: 22.5 g MgSO4.7H20 in 1 litre deionised water
Solution 4: 0.25 g FeO3.6H20 in 1 litre deionised water
20 ml of Solution 1 and 2 ml each of Solutions 2, 3 and 4 were diluted to 2 litres in the final culture medium.
- Additional substrate: None
- Solubilising agent (type and concentration if used): None
- Test temperature: 19 - 23°C
- pH: 7.00 - 7.61
- pH adjusted: yes
TEST SYSTEM
- Culturing apparatus: 2 litre Erleymeyer flasks
- Number of culture flasks/concentration: 1
- Measuring equipment: DOC Analyzer, pH meter, oximeter
- Test performed in closed vessels due to significant volatility of test substance: No
- Test performed in open system: Yes
- Details of trap for CO2 and volatile organics if used: None
SAMPLING
- Sampling frequency: 0, 3 h and every 3-4 days thereafter
- Sample pre-treatment: Samples were filtered through membrane filters which had previously been boiled in deionised water to remove any organic matter.
- Sample storage before analysis: None
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- None
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 5
- Sampling time:
- 8 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 14
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 39
- Sampling time:
- 21 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 61
- Sampling time:
- 28 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 94
- Sampling time:
- 42 d
- Details on results:
- The degradation value at day 28 was 61 % but no plateau was
yet reached, so that the test was prolonged for two further
weeks. After 42 days 94 % of EPTAC was degraded.
It was said that an abiotic degradation could not be observed and no bacterial toxicity was measured, but no raw data was presented.
BOD5 / COD results
- Results with reference substance:
- The control substance degraded to >99 % within 5 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- In an inherent biodegradation test conducted in accordance with OECD302B (with deviations), EPTAC attained 94% degradation in 42 days. The authors of the report therefore concluded that EPTAC can be described as inherently biodegradable. However, EU criteria for classification as inherently biodegradable were not met in this study.
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