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EC number: 206-114-9 | CAS number: 302-01-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Principles of method if other than guideline:
- EPA 600/3-75-009, US Environ Prot Agency, Corvallis, Oregon, 61 pp
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on test solutions:
- - stock solutions were prepared in soft reconstituted water
- reconstituted water was prepared by adding the following quantitees of salts to 1 L of deionized water: 48 mg NaHCO3, 30 mg MgSO4, 30 mg CaSO4, 2 mg KCl - Test organisms (species):
- Daphnia pulex
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- around 35 mg/L as CaCO3
- Test temperature:
- 20 °C
- pH:
- 7.1 - 7.2
- Dissolved oxygen:
- 8.2 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: immobilization
- Remarks on result:
- other: 0.16 - 0.23 mg/L (95 % CL)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: immobilization
- Remarks on result:
- other: 0.13 - 0.19 mg/L (95 % CL)
- Details on results:
- Analytic Monitoring:
Exposure Tank Hydrazine Concentratin mg/l
Test 1 Test 2
0 <0.002 +/- 0.000 <0.002 +/- 0.000
1 0.04 +/- 0.006 0.03 +/- 0.007
2 0.08 +/- 0.008 0.06 +/- 0.002
3 0.16 +/- 0.006 0.13 +/- 0.006
4 0.31 +/- 0.024 0.26 +/- 0.010
5 0.66 +/- 0.030 0.53
6 1.26 +/- 0.060 1.07
7 2.75 +/- 0.144 2.17 +/- 0.119
8 5.36 +/- 0.245 4.35
Summarizing these results, the hydrazine loss was minor during the daphnid static exposures. Mean hydrazine loss was 2.7 % for the 24 h-periode preceding and following renewal. Thus, the hydrazine concentration did not fall below 80 % of nominal concentrations justifying the use of nominal concentrations. - Conclusions:
- Hydrazine has to be scored as very toxic towards aquatic life.
- Executive summary:
For hydrazine an EC50(48 h) of 0.175 mg/L was observed with respect to the immobilization of daphnids (Velte, 1984).
Reference
EC50 related to hydrazine Since there is no significant difference between the two 48 h values (Velte, 1984), a mean EC50(48 h) can be calculated. EC50(48 h)= 0.175 mg/L
Description of key information
For hydrazine an EC50(48 h) of 0.175 mg/L (mean value of two separate tests) was observed with respect to the immobilization of daphnids (Velte, 1984), whereas exposure concentrations were proved by analytical measurements.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.175 mg/L
Additional information
Since there is no significant differnce between the two obtained 48 h values, a mean EC50(48 h) of 0.175 mg/L was used for the further assessment. Tests were conduced as a static renewal test, whereas initial hydrazine exposure concentrations were measured at 0 and 24 h, and the renewal concentrations were measure at 24 and 48 h by the means of a colorimetric method. The hydrazine loss was minor during the daphnid static exposures. Mean hydrazine loss was 2.7 % for the 24 h-period preceding and following renewal. Thus, the hydrazine concentration did not fall below 80 % of nominal concentrations justifying the use of nominal concentrations.
The study performance is comparable to current guidelines but having one minor drawback concerning the study documentation. No information is presented with respect to the mortality in control samples as an indication of stress or illness independent of the presence of the test item. Nevertheless, the results were used for the hazard assessment as the obtained results would rather overestimate the toxicity of the test item in the case that other factors contributed to the observed effects (stress, illness). Consequently, results would be more protective with respect to aquatic hazard assessment.
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