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EC number: 701-385-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- from 5/12/2002 to 20/03/2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Commission Directive 87/302/EEC
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ar-nonyldiphenylamine
- EC Number:
- 248-295-7
- EC Name:
- Ar-nonyldiphenylamine
- Cas Number:
- 27177-41-9
- Molecular formula:
- C21H29N
- IUPAC Name:
- 4-nonyl-N-phenylaniline
- Test material form:
- liquid: viscous
- Remarks:
- pale brown viscous liquid
- Details on test material:
- Reaction products of Benzenamine, N-phenyl, and Nonene, branched EC 253-249-4; CAS no.: 36878-20-3, followed by purification (distillation) with a 96% mono-alkyl content.
impurity 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, dark
- Solubility in water: 0.0113 mg/l
- Reactivity of the test material with the incubation material used (e.g. plastic ware): Data suggested that the test material would adsorb to glassware (glass bottles were pre-conditioned with test concentration)
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding): two different methods were used. First: test material was dispersed directly in water; Second: test material was dissolved in dimethylformamide first
- Final concentration of a dissolved solid, stock liquid or gel: 2.825 mg/25 ml (second)
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - Sampling method: after 30 min an aliquot was removed from the colonial flasks of each treatment and poured into a measuring vessel (250 ml , darkened glass BOD bottle). The respiration rate was measured while the content of the vessel was stirred constantly. It was returned to the test vessel after measurement
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
a) test material was dispersed directly in water and subjected to ultrasonication (30 min), sythetic sewage, activated sewage sludge and water were added, final concnetration = 1000 mg/l ;
b) a preliminary solution in dimethylformamide was prepared (2.825 mg/ 25 ml), 50 µl of the stock solution was dispersed with synthetic sewage, activated sewage sludge and water, final conmcentration = 0.0113 mg/l
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethylformamide
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): solvent control = 100 µl/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Oil globules of test material dispersed throughout with an oil slick on the surface were observed for the test concentration of 1000 mg/l
- Other relevant information: activated sewage sludge was centrifuged (3500 rpm, 10 min) and supernatant discarded prior to addition of activated sewage sludge "pellets" to the test vessels
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: on collection day
- Name and location of sewage treatment plant where inoculum was collected: aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK
- Method of cultivation: maintenance under continuous aeration at 21 °C
- Preparation of inoculum for exposure: a) effect of dispersed material: pH= 7.5; determination of suspended solid level = sample was filtered (100 ml) through a pre - weighted GF/A filter paper and dried
b) effect at water solubility level: pH = 7.7; same procedure as in a)
- Initial biomass concentration: 4.0 g/l
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 100 mg/l [CaCO3 ] (hardness of water used for preparation of the test medium)
- Test temperature:
- room temperature = 21 °C
- pH:
- see table 1
- Dissolved oxygen:
- see table 1
- Nominal and measured concentrations:
- Test material nominal: 1000 mg/l (dispersed concentration), 0,0113 mg/l
Reference substance nominal: 3.2, 10, 32 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 ml colonial flask
- Type (delete if not applicable): not specified
- Material, size, headspace, fill volume: fill volume = 500 ml
- Aeration: every 15 min with compressed air (approx. 0.5 - 1 l/min)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 1
- No. of vessels per reference substance (replicates): 1per concentration (3 in total)
- Sludge concentration (weight of dry solids per volume): 800 mg/ 500 ml
- Nutrients provided for bacteria: Synthetic sewage = 16 g Peptone, 11 g Meat extract, 3 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4 dissolved in 1 l water
- Nitrification inhibitor used (delete if not applicable): none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory tap water was dechlorinated, filtered through an activated carbon filter and partly softened, the water was warmed to the required temperature prior use
OTHER TEST CONDITIONS
- Light intensity: normal laboratory lightning
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : pH value and oxygen consumption rate was measured at the end of the exposure, changes on the test preparations were observed throughout the test period
TEST CONCENTRATIONS
- Justification for using fewer concentrations than requested by guideline: results of the range finding study and preliminary dosing trials
RANGE FINDING STUDY
- Test concentrations: 100, 1000 mg/l and control
- Results used to determine the conditions for the definitive study: No significant effect on respiration was observed at either test concentration employed. Results of the preliminary dosing trials considered 0.0113 mg/l to be the highest employed concentration, which is consistent with the reported water solubility level of the test material. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.011 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: concentration was equal to the reported water solubility level of the test material
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.011 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: concentration was equal to the reported water solubility level of the test material
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: concentration exceeded the water solubility of the test material
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: concentration exceeded the water solubility of the test material
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: oil slick on the surface for 1000 mg/l treatment
- Effect concentrations exceeding solubility of substance in test medium: yes (1000 mg/l)
- Blank controls oxygen uptake rate:
a) (1000 mg/l) 0.5 - 0.53 mg O2/l/min
b) (0.0113 mg/l) 0.64 - 0.65 mg O2/l/min
- Coefficient of variation of oxygen uptake rate in control replicates:
a) (1000 mg/l) after 30 min = +- 1%, after 3 h = +- 3 %
b) (0.0113 mg/l) after 30 min = +- 1%, after 3 h = +- 1 % - Results with reference substance (positive control):
- - Results with reference substance valid?: yes
- Relevant effect levels:
EC50 after 3 h = 12 mg/l (reported for dispersed test material 1000 mg/l)
EC50 after 3 h = 13 mg/l (reported for water solubility level 0.0113 mg/l)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results show that at a concentration in excess of the water solubility level of the test material, the test material has no effect on the respiration rate of the activated sewage sludge. Furthermore, at a concentration equivalent to the reported water solubility level of the test material, the test material has no effect on the respiration rate of the activated sewage sludge.
- Executive summary:
A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 209 "Activated Sludge, Respiration Inhibition Test". A range-finding test conducted at test concentrations in excess of the water solubility of the test material (100 and 1000 mg/l) indicated that dispersed test material exhibited no adverse effect on the respiration rate of activated sewage sludge.
Therefore in order to satisfy the requirements of all Competent Authorities worldwide, the definitive test was conducted using two methods of addition of the test material to the test system:
a) activated sewage sludge was exposed to a dispersed test material concentration of 1000 mg/l, which was dispersed directly in water
b) acitive sewage sludge was was exposed to a nominal test concentration of 0.0113 mg/l, which is consitent with the repoted water solubility level of the test material. The concentration was prepared using a preliminary solution in auxiliary solvent to spike the test medium.
In each method the activated sewage sludge was exposed to the test material for a period of 3 hours at 21ºC with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 30 min and 3 h contact time and compared to data for the control and reference material, 3,5-dichlorophenol. In the test vessels of both methods no significant inhibition of respiration of the activated sewage sludge was observed.
3-Hour EC50 value was determined to be greater than 1000 mg/l. Correspondingly the No Observed Effect Concentration (NOEC) after 3 hours exposure was determined to be 1000 mg/l. These results show that at a concentration in excess of the water solubility level of the test material, the test material has no effect on the respiration rate of the activated sewage sludge.
In the test vessels prepared at a concentration equivalent to the reported water solubility level of the test material, no significant inhibition of respiration of the activated sewage sludge was observed. The 3-h EC50 value was determined to be > 0.0113 mg/l, the NOEC was determined to be 0.0113 mg/l. These results show that at a concentration equivalent to the reported water solubility level of the test material, the test material has no effect on the respiration rate of the activated sewage sludge.
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