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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The acute toxicity to Daphnia magna STRAUS expressed in terms of effective loadings (EL0, 48 h) lead to an EL0 of >= 100 mg/L and 25 mg/L for HDI trimer, respectively. After 48 h the EL50 for HDI trimer was 127 mg/L. The studies were conducted according to Directive 92/69/EEC, C2. The results relate to the nominal concentration.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
127 mg/L

Additional information

The 48–hr acute toxicity of HDI trimer to Daphnids (Daphnia magna) was studied under static conditions. The experiment was carried out in accordance with the EU Method C.2. The medium composition was compliant with the Guideline requirement. Considering the variable composition of the substance, a Water Accomodated Fraction (=WAF) procedure was used (ratio: 100 mg of the test item was added to 1 L of water). The results are expressed in terms of effective loadings (EL). Immobilization was observed daily. Mobility was recorded daily in, control and treated groups . No immobiles were observed after 24h in any groups. After 48 hours, 4 of 20 daphnids were immobile (20 %) in the 50 mg/L group and 7 daphnids in the 100 mg/L group (35 %). In the test report, no EL50 was given, but an EL100 > 100mg/L. The EL50 was later (in the year 2010) extrapolated and calculated using TOXRAT (EL50=127 mg/L).

Disregarded study: For Daphnia magna STRAUS an EC 0 of >= 100 mg/L was obtained after 24 hours. The study was conducted according to the German standard method „UBA- Verfahrensvorschlag: Bestimmung der Schwimmunfähigkeit beim Wasserfloh (Daphnia magna), EC0/EC50/EC100, 24 Std. statisches System“. The result relates to the nominal concentration. This study is not regarded as being reliable. Even if the validity criteria are fulfilled according to the validity criteria of 1989 the study design does not meet the criteria of today standard methods. According to international standard methods an exposure time of 48 hours follows the state-of-the-art of science.