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EC number: 200-860-9 | CAS number: 75-31-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.1150 (Acute inhalation toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
Test material
- Reference substance name:
- Isopropylamine
- EC Number:
- 200-860-9
- EC Name:
- Isopropylamine
- Cas Number:
- 75-31-0
- Molecular formula:
- C3H9N
- IUPAC Name:
- propan-2-amine
- Test material form:
- liquid
- Details on test material:
- Isopropylamine [CAS No. 75-31-0]; supplied by Hoechst Celanese Corp. (C-899; 100% active ingredient)
purity > 99%, for further details, please see statement on test material purity for older studies on isopropylamine, attached in IUCLID section 13.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Raleigh, North Carolina, USA
- Age at study initiation: males 8 - 9 wk, females 10 - 11 wk
- Weight at study initiation: males: 242 g - 335g, females: 208g - 236g
- Housing: doubly housed during acclimation period, individually housed in Plexiglass chamber (100 L) during exposure
- Diet , water: ad libitum
- Acclimation period: 1 to 3 wk
ENVIRONMENTAL CONDITIONS during acclimation period
- Temperature (°C): 19 - 24°C
- Humidity (%): 40 - 70%
- Air changes (per hr): no data
- Photoperiod: 12hrs dark / 12hrs light)
ENVIRONMENTAL CONDITIONS during exposure
- Temperature (°C): 21 - 26°C
- Humidity (%): 46 - 65%
- Air flow rate: 20 L/min (= complete air change every 5 min)
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Plexiglass exposure chamber with glass front
- Exposure chamber volume: 100 L
- Method of holding animals in test chamber: individually
- Source and rate of air: 20 L/min, the air exchange was about once per every 5 min.
- Method of conditioning air: by passing air through a midget impinger containing the TS
- System of generating particulates/aerosols:
- Temperature: actual 21-26°C
- Relative humidity: 64-65%
- pressure: no information given
TEST ATMOSPHERE
- Brief description of analytical method used: MIRAN Ambient Air Analyser; test atmosphere was drawn through Balston DFU filter via Teflon line into the Analyser
- Samples taken from breathing zone: yes (hourly sampling)
- Particle size measurements were performed at hourly intervals - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 5.3, 2.8, 5.1, and 6.5 mg/L (analytical) [7.1, 3.4, 5.9, and 7.2 mg/L (nominal)], no aerosol
The nominal concentration was calculated from the consumption of TS from the reservoir divided by the total amount of air. - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days; detailed observations once daily for survivers; Assessment of viability twice daily
- Necropsy of survivors performed: yes (at post-exposure day 15)
- Examinations of dead and surviving animals of nasal passages, trachea, orifices, cranial cavity, brain and spinal cord, viscera, cervical tissues and organs - Statistics:
- Calculation of median lethal concentration, 95% CL (method according to Litchfield and Wilcoxon)
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- ca. 8.7 mg/L air (analytical)
- 95% CL:
- >= 6.5 - <= 12
- Exp. duration:
- 4 h
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- ca. 3 540 ppm
- Exp. duration:
- 4 h
- Remarks on result:
- other: calculated from formula 1 ppm = 2.46mg/m^3
- Key result
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 7.7 mg/L air (analytical)
- Exp. duration:
- 4 h
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- ca. 9.4 mg/L air (analytical)
- Exp. duration:
- 4 h
- Mortality:
- see table below
- Clinical signs:
- other: Labored breathing, lacrimation, nasal discharge, reduced activity, closed eyes. Respiratory distress and nasal discharge persisted the first week of post-exposure, but moist and dry rales were observed in most of the survivors throughout 14 d.
- Body weight:
- Transient losses were prominent.
- Gross pathology:
- Nasal tubinates and lungs were reddened and swollen with lung emphysema present in a few animals.
Any other information on results incl. tables
Group Mean Exposure Nominal [mg/L] Mortalitytration Concentration No.dead/No.exposed
---------------------------------------------------------------- I* 5.3 7.1 5/5 2/5 7/10 II 2.8 3.4 0/5 0/5 0/10 III 5.1 5.9 2/5 0/5 2/10 IV 6.5 7.2 1/5 2/5 3/10 ================================================================ * Group I was excluded from evaluation due to technical problems during exposure The LD50 of 8.7 mg/L (95% C.I. = 6.5 - 12 mg/L) is the combined extrapolated value.
-----------------
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- Under the conditions of this study, the mean LC50 in male and female rats was 8.7 mg/L (8700 mg/m3)
- Executive summary:
The study was designed to assess the acute inhalation toxicity (median lethal concentration). Groups of 5 rats per sex were exposed to 5.3, 2.8, 5.1, and 6.5 mg/L (analytical) [7.1, 3.4, 5.9, and 7.2 mg/L (nominal)] of test substance vapour for 4 h. The post-exposure observation period was 14 days. The LC50 for combined sexes was 8.7 mg/L. Clinical signs during exposure were laboured breathing, lacrimation, nasal discharge, reduced activity, closed eyes. Respiratory distress and nasal discharge persisted the first week of post-exposure, but moist and dry rales were observed in most of the survivors throughout 14 d. Significant body weight losses were evident following exposure, but recovery occured over time. No clear treatment-related postmortem findings were observed.
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