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EC number: 200-855-1 | CAS number: 75-26-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with GLP and appropriate OECD Guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- 2-bromopropane
- EC Number:
- 200-855-1
- EC Name:
- 2-bromopropane
- Cas Number:
- 75-26-3
- Molecular formula:
- C3H7Br
- IUPAC Name:
- 2-bromopropane
- Details on test material:
- - Name of test material (as cited in study report): Iso-Propyl Bromide
- Physical state: Colourless liquid
- Analytical purity: > 99%
- Lot/batch No.: 1-302-1
- Storage condition of test material: Room temperature and protected from light
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Test Animals
Species, strain: rat, Sprague-Dawley ICO: OFA-SD (lOPS Caw).
Reason for this choice: rodent species commonly requested by the international regulations for this type of study.
Breeder: Iffa Credo, 69210 L'Arbresle, France.
Number and sex: one group of 10 animals (5 males and 5 females).
Age/weight: on the day of treatment, the animals were approximately 6 weeks old, and had a mean body weight of 176 ± 5 g for the males and 151 ± 5 g for the females.
Acclimatization: at least 5 days before the beginning of the study.
Identification of the animals: the animals were identified individually by earmarks or earnotches.
Environmental Conditions
During the acclimatization period and during the main test, the conditions in the animal room were as follows:
temperature : 22 ± 3°C
relative humidity: 50 ± 20%
light/dark cycle: 12 h/12 h
ventilation: about 13 cycles/hour of filtered, non-recycled air.
The temperature and relative humidity were recorded continuously and records retained.
The housing conditions (temperature, relative humidity, light/dark cycle and ventilation) were checked monthly.
The animals were housed in polycarbonate cages (48 x 27 x 20 cm) covered with a stainless steel lid . Each cage contained 4 to 7 animals of the same sex during the acclimatization period and 5 rats of the same sex during the treatment period. Each cage contained graded, dust-free sawdust (SICSA, 94142 Alfortville, France).
Bacteriological analysis of the sawdust and detection of possible contaminants (pesticides, heavy metals) are performed periodically.
Food and water
All the animals had free access to A04 C pelleted diet (U.A.R., 91360 Villemoisson-sur-Orge, France). Each batch of food was analysed (composition and contaminants) by the supplier.
Drinking water filtered by a F.G. Millipore membrane (0.22 micron) was contained in bottles.
There were no contaminants in the diet, water or sawdust at levels likely to have influenced the outcome of the study.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 2,000 mg/kg
- No. of animals per sex per dose:
- 5 male/5 female
- Control animals:
- no
- Details on study design:
- The administration was performed in a single dose by oral gavage. The volume administered to each animal was adjusted according to body weight determined on the day of treatment. The single administration was performed on Day 1 and was followed by a 14-day observation period.
The animals were observed frequently during the hours following administration of the test substance, for detection of possible treatment-related clinical signs. Observation of the animals was made at least once a day for a period of 14 days, to determine whether any of the clinical
signs were reversible or not. Clinical signs were recorded for each animal individually.
The animals were checked frequently during the hours following administration of the test substance for mortality or signs of morbidity, then at least twice a day thereafter.
The animals were weighed individually just before administration of the test substance, and then on days 5, 8 and 15 for the surviving animals.
The body weight gain of the treated animals was compared to a reference curve of C.I.T. control animals with the same initial weight.
On day 15, the animals were sacrificed by CO2 inhalation in excess and a macroscopic examination was performed.
After opening the thoracic and abdominal cavities, a macroscopic examination of the main organs (digestive tract, heart, kidneys, liver, lungs, pancreas, spleen and any other organs with obvious abnormalities) was performed. In case of macroscopic lesions, organ samples were taken and preserved in 10% buffered formalin. No microscopic examination was performed.
Evaluation of the innoxiousness or toxicIty of the test substance following a single oral administration in rats should include the relationship, if any, between the animals' exposure to the test substance and the incidence and severity of all abnormalities including behavioural and clinical abnormalities, macroscopic lesions, body weight changes, mortality and any other toxic effects.
Results and discussion
- Mortality:
- No deaths occurred during the observation period.
- Clinical signs:
- other: Hypoactivity (slight and marked), ataxia and piloerection were noted within 4 hours post-treatment. No clinical signs were observed thereafter.
- Gross pathology:
- Macroscopic examination of the main organs of the animals sacrificed at the end of the study revealed no apparent abnormalities.
Any other information on results incl. tables
Table 1 -- Individual Clinical Signs and Mortality | |||||||||
Dose (mg/kg) | Time | Males | Females | Clinical Signs | |||||
2000 | 15 min | 01-02-03-04-05 | 01-02-03-04-05 | hypoactivity | |||||
30 min | 01-02-03-04-05 | 01-02-03-04-05 | Sedation | ||||||
1 hr | 04 | 02-05 | Sedation, ataxia | ||||||
1 hr | 01-02-03-05 | 01-03-04 | Sedation | ||||||
2 hr | 01-02-03-04-05 | 01-02-03-04-05 | Sedation, piloerection | ||||||
4 hr | 01-02-03-04-05 | 01-02-03-04-05 | None | ||||||
Days 2 to 15 | 01-02-03-04-05 | 01-02-03-04-05 | |||||||
Table 2 -- Individual Body Weight and Body Weight Change of Treated Rats (g) | |||||||||
Dose | Sex | Animal No. | Days | ||||||
mg/kg | 1 | (1) | 5 | (1) | 8 | (1) | 15 | ||
2000 | Male | 01 | 184 | 50 | 234 | 27 | 261 | 58 | 319 |
02 | 72 | 46 | 218 | 30 | 248 | 49 | 297 | ||
03 | 173 | 52 | 225 | 28 | 253 | 62 | 315 | ||
04 | 175 | 52 | 227 | 22 | 249 | 61 | 310 | ||
05 | 175 | 43 | 218 | 26 | 244 | 60 | 304 | ||
2000 | Female | 01 | 159 | 43 | 202 | 13 | 215 | 27 | 242 |
02 | 148 | 44 | 192 | 16 | 208 | 18 | 226 | ||
03 | 148 | 45 | 193 | 0 | 193 | 16 | 209 | ||
04 | 147 | 26 | 173 | 14 | 187 | 20 | 207 | ||
05 | 152 | 57 | 209 | 7 | 216 | 10 | 226 | ||
(1) = Body weight gain | |||||||||
Table 3 - Individual Macroscopic Post-Mortem Examinations | |||||||||
Dose (mg/kg) | Time | Males | Females | Macroscopic Abnormalities | |||||
2000 | Day 15 | 01-02-03-04-05 | 01-02-03-04-05 | None |
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- Under the experimental conditions described in this study, the LDo of the test substance Iso-Propyl Bromide when administered by oral route in rats was higher than 2,000 mg/kg.
- Executive summary:
In a CoR 1 acute oral toxicity study, five male and five female Sprague-Dawley rats were administered a single dose of 2,000 mg/kg bw 2 -bromopropane (>99% purity) and were observed for 14 days following exposure. No deaths occurred as the result of exposure. No lasting clinical signs were noted. Bodyweight gain was unaffected. No apparent aabnormalities to organs or tissues were noted on gross pathology.
Oral LD0 Males = > 2,000 mg/kg bw
Females = > 2,000 mg/kg bw
Combined = > 2,000 mg/kg bw
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