Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 243-900-0 | CAS number: 20592-85-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
- Principles of method if other than guideline:
- Application of radiolabelled substance and observation of absorption and excretion in urine.
- GLP compliance:
- no
- Remarks:
- pre-GLP
Test material
- Reference substance name:
- Nitrilotrimethylenetris(phosphonic acid)
- EC Number:
- 229-146-5
- EC Name:
- Nitrilotrimethylenetris(phosphonic acid)
- Cas Number:
- 6419-19-8
- Molecular formula:
- C3H12NO9P3
- IUPAC Name:
- [nitrilotris(methylene)]tris(phosphonic acid)
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- 5 male (208-222 grams) and 5 female (171-215 grams) Wistar rats
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Duration of exposure:
- 48 hours
- Doses:
- 200 µl (0.2 mL) of a 0.88% aqueous solution (pH 7) of 14C-ATMP (equivalent to 1.76 mg ATMP/ rat; 27.1 µCi/rat) was applied to 10 cm² area of shaved skin (24 hours prior to application) under occlusion for 48 hours.
The doses were based upon a mean body weight of 216.4 g for males and 186.4 g for females, equivalent to 8.1 or 9.4 mg/kg bw, respectively. - No. of animals per group:
- 5 males, 5 females
- Details on study design:
- PREPARATION OF DOSING SOLUTION
The test sample was prepared by custom synthesis 8.50 mg 14C-ATMP (540 µCi) was mixed with 26.0 mg unlabelled material and 3954 mg double distilled water. The final concentration of ATMP in the dosing solution appears to be 0.89% and not 0.88% as stated in the report.
It is unclear how the solution was neutralised to pH 7.0; use of NaOH may have resulted in the formation of the sodium salt.
SAMPLE COLLECTION
Radioactivity present in urine, faeces, exhaled air (14C-carbon dioxide), skin (application site), carcass and cage wash was quantified over a 48 hours post-application period.
Results presented as mean and SD.
Results and discussion
- Absorption in different matrices:
- The majority of the dose remained at the application site 48 hours after application, with trace amounts excreted in urine, faeces and as 14C-carbon dioxide or remaining in the carcass:
- Urine: 0.2-0.5%
- Faeces: 0.087-0.03%
- CO2: 0.087-0.061%
- Skin (application site): 76.3-60.2%
- Carcass: 0.22-0.35% (Mean data for males or females, respectively). The total absorbed over 48 hours was 0.603% of the dose in males, and 0.940% of the dose in females.
Of the material absorbed:
- urinary excretion was virtually complete during the first 24 hours post-treatment (93-95% of total present in urine) with negligible excretion between 24-48 hours;
- faecal elimination was comparable over 0-24 hours and 24-48 hours (approximately 50% of total excreted present at each time point);
- slightly more 14C-CO2 was exhaled over 0-8 hours (40-50% of total excreted) than over 8-24 hours or 24-48 hours.
Percutaneous absorptionopen allclose all
- Time point:
- 48 h
- Dose:
- 200 µl of a 0.88% aqueous solution (pH 7) of 14C-ATMP
- Parameter:
- percentage
- Remarks:
- male rats
- Absorption:
- 0.603 %
- Time point:
- 48 h
- Dose:
- 200 µl of a 0.88% aqueous solution (pH 7) of 14C-ATMP
- Parameter:
- percentage
- Remarks:
- female rats
- Absorption:
- 0.94 %
Any other information on results incl. tables
Absorption: 0.603% (male); 0.94% (female)
Excretion: the majority of the absorbed radiolabelled ATMP was excreted in the urine within 24 hours of treatment.
Applicant's summary and conclusion
- Conclusions:
- In a dermal absorption study, not conducted according to any OECD Test Guideline and pre-GLP (Reliability 2), the cutaneous absorption of radiolabelled ATMP over 48 hours was 0.603% in male rats and 0.94% in females. 60-75% of the applied material was recovered from the application site at 48 hours. Radioactivity was excreted mainly in urine over the first 24 hours following application.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)