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Diss Factsheets
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EC number: 202-576-0 | CAS number: 97-36-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- methaemoglobinaemia
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented study report which meets basic scientific principles. Study was conducted priore to GLP implementation.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: company guideline
- Deviations:
- no
- Principles of method if other than guideline:
- Test animal: cat
Route of exposure: gavage
Examinations/observations: Clinical signs, lethality, Methemoglobin concentration - GLP compliance:
- no
- Remarks:
- prior to GLP implementation
- Type of method:
- in vivo
- Endpoint addressed:
- acute toxicity: oral
Test material
- Reference substance name:
- 2',4'-dimethylacetoacetanilide
- EC Number:
- 202-576-0
- EC Name:
- 2',4'-dimethylacetoacetanilide
- Cas Number:
- 97-36-9
- Molecular formula:
- C12H15NO2
- IUPAC Name:
- 2',4'-dimethylacetoacetanilide
- Details on test material:
- - Name of test material (as cited in study report): Acetessig-m-xylidid
Constituent 1
Test animals
- Species:
- cat
- Strain:
- not specified
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on exposure:
- VEHICLE
- Concentration in vehicle: 2.0 or 0.5 % (aqueous suspension) - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- observation period: 14 d
- Frequency of treatment:
- once
- Post exposure period:
- 14 d
Doses / concentrations
- Remarks:
- Doses / Concentrations:
200 or 50 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 200 mg/kg bw: 2 male, 4 female
50 mg/kg bw: 1 male, 1 female - Control animals:
- other: blood sampling before treatment
Examinations
- Examinations:
- Clinical signs, lethality, Methemoglobin concentration
- Positive control:
- no
Results and discussion
- Details on results:
- 200 mg/kg bw:
Lethality: 3/6
MetHb concentration: Slightly increased except one surviving animal which showed a distinct increase
Clinical signs: Apathy, salivation, ataxic gate, crying, prone position, lateral position, decreased feed consumption
50 mg/kg bw:
Lethality: 0/2
MetHb concentration: Very slightly increased
Clinical signs: no abnormal findings
Any other information on results incl. tables
Dose |
Animal no / sex |
Fate |
Methemoglobin concentration (%) |
||
Before application |
4 h post apllication |
3 d post application, cardiac aspiration |
|||
200 mg/kg bw |
70 / m |
survived |
0.0 |
2.1 |
/ |
88 / f |
died |
0.0 |
8.6 |
0.0 |
|
12 / m |
died |
0.8 |
6.3 |
0.6 |
|
24 / f |
survived |
0.9 |
3.1 |
/ |
|
18 / f |
died |
0.6 |
2.9 |
0.1 |
|
23 / f |
survived |
0.5 |
50.7 |
/ |
|
50 mg/kg bw |
82 / m |
survived |
0.0 |
1.6 |
/ |
62 / f |
survived |
0.0 |
1.3 |
/ |
Applicant's summary and conclusion
- Conclusions:
- The oral LD50 for cat approximately amounts to 200 mg/kg bw. Lethality was not caused by MetHb formation.
- Executive summary:
In order to assess the methemoglobin-forming potential of Acetessig-m-xylidid male and female cats received single oral doses of 50 or 200 mg/kg bw. Blood samples were taken before and after treatment.
In the high dose group cats showed apathy, salivation, ataxic gate, crying, prone position, lateral position, and decreased feed consumption. MetHb concentrations were slightly increased. 3 of 6 animals died.
In the low dose group no abnormalities were observed. MetHb concentration was very slightly increase. All animals survived.
Based on that the oral LD50 approximately amounts to 200 mg/kg bw. and lethality was not considered to be caused by MetHb formation.
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