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EC number: 213-934-0 | CAS number: 1067-53-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Cited as Directive 92/69/EEC, C.3
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The Dissolved Organic Carbon (DOC) concentration of the stock solution used to prepare the test media was determined.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was added to the dilution medium at 1.2 g/L and stirred for 18 hours. After that the solution was filtered and the DOC determined. The Total Organic Carbon (TOC) infrared analyzer was used. The DOC of the solution was 588 mg/L, this corresponds to a test material concentration of 1247 mg/L. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81.SAG
- Source (laboratory, culture collection): Institute for Water, Ground and Air Hygiene, Berlin (Germany)
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: A pre-culture is produced from an original culture by super-inoculation three days before the test begins. From this culture the test cultures are inoculated at a density of about 20000 cells/ml. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- No data
- Test temperature:
- 24 +/-1ºC
- pH:
- 8.2-8.4 at start of test
7.6-8.9 at end of test - Dissolved oxygen:
- Not reported
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal test concentrations (based on measured DOC concentration in stock solution converted to the equivalent test substance concentration): 0 (Control), 75, 125, 212, 374, 624 and 1122 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open Erlenmeyer flasks
- Material: glass
- Aeration: none
- Initial cells density: 20000 cells/ml
- Control end cells density: 560000 cells/ml
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: start and end of test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 8000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Algal cell counts were made photometrically at 0, 24, 48 and 72 hrs.
- Determination of cell concentrations: spectrophotometer (absorption at 685 nm)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7-1.8 - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 304 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 136 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 611 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Reported statistics and error estimates:
- EC50 values were determined by Probit Analysis
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72-hour ErC50 value of 611 mg/L and NOErC of 75 mg/L have been determined for the effects of the test substance on growth rate of Scenedesmus subspicatus (new name: Desmodesmus subspicatus), based on nominal concentrations. Due to the rapid hydrolysis half life of the substance. is likely that the test organisms were primarily exposed to the hydrolysis products of the substance. These results are equivalent to ErC50 231 mg/L and NOErC 28 mg/L when expressed in terms of the silanol hydrolysis product.
Reference
Table 1. Test results
Nominal concentration (mg/L) | Nominal cell concentration at start of test (cells/mL) | Mean cell concentration after 24 hours (cells/mL) | Mean cell concentration after 48 hours (cells/mL) | Mean cell concentration after 72 hours (cells/mL) | Percentage reduction in biomass at end of the test* | Percentage reduction in 0 -72 -hour growth rate* |
0 (Control) | 20000 | 60000 | 220000 | 670000 | - | - |
75 | 20000 | 60000 | 220000 | 690000 | -1.8 | -0.8 |
125 | 20000 | 60000 | 180000 | 490000 | 23 | 9.0 |
212 | 20000 | 50000 | 150000 | 290000 | 47.8 | 23.9 |
374 | 20000 | 50000 | 140000 | 180000 | 59.3 | 37.5 |
624 | 20000 | 50000 | 110000 | 140000 | 68.1 | 44.6 |
1122 | 20000 | 140000 | 320000 | 410000 | -8.8 | 14 |
*A negative sign (-) indicates that biomass
or growth rate was higher than in the Control.
Description of key information
Toxicity to aquatic algae: 72 hour ErC50 611 mg/L, ErC10 136 mg/L and NOEC 75 mg/L (nominal concentration based on measured DOC of stock solution) (EU method C.3). The ErC50 is equivalent to 231 mg/L, the ErC10 is equivalent to 51 mg/L and the NOEC is equivalent to 28 mg/L when expressed in terms of the silanol hydrolysis product.
The reported 72 hour EC50 and EC0 for 2-methoxyethanol for freshwater algae are 25,500 mg/L and 8,900 mg/L for Pseudokirchneriella subcapitata, respectively.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 231 mg/L
- EC10 or NOEC for freshwater algae:
- 28 mg/L
Additional information
A 72-h ErC50 value of 611 mg/L, ErC10 of 136 mg/L and NOEC of 75 mg/L (nominal) have been determined for the effects of tris(2-methoxyethoxy)(vinyl)silane on growth rate of Scenedesmus subspicatus (new name: Desmodesmus subspicatus). In view of the test media preparation method/exposure regime it is likely that the test organisms were exposed predominantly to the hydrolysis products of the tested substance.
The results may be expressed in terms of concentration of the hydrolysis product, vinylsilanetriol, by applying a molecular weight correction:
(MW of silanol = 106.15 / MW of parent = 280.40) * 611 mg/l = ErC50 231 mg/L,
(MW of silanol = 106.15 / MW of parent = 280.40) * 136 mg/l = ErC10 51 mg/L
and (MW of silanol = 106.15 / MW of parent = 280.40) * 75 mg/l = NOEC 28 mg/L.
This study was assigned a reliability score of 1 because it is a guideline study (EU Method C.3) conducted in accordance with GLP.
A 72-hour EC50 and NOEC of 10,000 mg/l and 4,100 mg/l (nominal), respectively, have been determined for the effects of 2-methoxyethanol on the growth rate ofPseudokirchneriella quadricauda,in a reliable study conducted according to ISO water quality guideline 8692 (Devillers et al., 2002).
Refer to IUCLID Section 6 endpoint summary for further discussion of the approach to chemical safety assessment for this registration substance.
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